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Shared Service

Large-scale research equipment and laboratories / Imaging Systems

Confocal Microscope

15. AMBIO Nikon Upright Spinning Disk Confocal S2

Carrier: Charité

Usable by: HU | TU | FU | Charité

Imaging modes

  • Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
  • Upright

Lasers and max. intensities out of 25x objective

  • 405 nm : 6.3 mW
  • 488 nm : 7.7 mW 
  • 561 nm : 9.7 mW 
  • 647 nm : 9.5 mW

Detectors 

  • Zyla 4.2 Plus sCMOS camera (2048×2048 pixels, 40 fps@16-bit, 53 fps@12-bit)

Emission Filters

Filter

λ/Bandwidth

Quad emission filter (DAPI, GFP, mCherry, Cy5)

440/20

521/20

607/20

700/20

DAPI

447/60

GFP

525/25

mCherry

600/52

Cy5

708/75

Objectives

Mag.

Type

Immer.

NA

WD [μm]

Info

10x

Plan Achroat

Water

0.45

4,000

λ

25x

Apo

water

1.10

2,000

NCC

Special

  • Andor Revolution SD System (CSU-X1)
  • Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
  • Ultra-fast laser switching/ tuning (triggered acquisition)

Further information:

https://bua.openiris.io/Landing/Res...
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Possible user: No restrictions

Conditions of use:

The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

 

Note

New users – access upon training request here.

Email: ambio@charite.de

Provider:

Advanced Medical BIOimaging Core Facility

Provider Location:

Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany

Location:

Charité Campus Mitte
CCO Rm. 06-307
Virchowweg 6
10117 Berlin
Germany

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