Confocal Microscope

Imaging modes

Lasers and max. intensities out of 60x oil objective

Emission Filters

Filter	λ/Bandwidth
Quad emission filter (DAPI, GFP, mCherry, Cy5)	440/40 521/21 607/34 700/45
DAPI	452/45
GFP	525/50
mCherry	600/50
Cy5	685/40
GFP narrow	510/20

Detectors

2 EMCCD cameras (iXon3 DU-888 Ultra, 1024×1024 pixels, 13 μm pixel size, 26 fps full frame, usable area ~512×512 pixels, 93 fps)
Current dual color dichroic for simultaneous GFP & mCherry detection

Objectives

Special

Andor Revolution SD System (CSU-X)
Uniform spinning disk illumination
2 cameras for ultra-fast dual color imaging
Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
Ultra-fast laser switching/ tuning (triggered acquisition)

Further information:

open all

Possible user: No restrictions

Conditions of use:

https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2024-01-01.pdf