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Large-scale research equipment and laboratories / Imaging Systems

Confocal Microscope

13. AMBIO Nikon Spinning Disk Confocal CSU-X

Carrier: Charité

Usable by: HU | TU | FU | Charité

Imaging modes

  • Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
  • FRAP/ PA (photo activation/ conversion)
  • Live-cell incubator (temperature, CO2, humidity)

Lasers and max. intensities out of 60x oil objective

  • 405 nm : 3.54 mW
  • 488 nm : 3.85 mW 
  • 561 nm : 4.11 mW 
  • 647 nm : 4.68 mW

Emission Filters

Filter

λ/Bandwidth

Quad emission filter (DAPI, GFP, mCherry, Cy5)

440/40

521/21

607/34

700/45

DAPI

452/45

GFP

525/50

mCherry

600/50

Cy5

685/40

GFP narrow

510/20

Detectors

  • 2 EMCCD cameras (iXon3 DU-888 Ultra, 1024×1024 pixels, 13 μm pixel size, 26 fps full frame, usable area ~512×512 pixels, 93 fps)
  • Current dual color dichroic for simultaneous GFP & mCherry detection

Objectives

Mag.

Type

Immer.

NA

WD [μm]

Info

10x (not installed)

Plan Achroat

Air

0.3

10,500

  

20x

Plan Apo

Air

0.8

1,000

DIC N2 VC

40x

Apo

Water

1.25

180

DIC N2 λ S

60x

Plan Apo

Water

1.27

170

DIC N2 IR

40x

Plan Flour

Oil

1.3

200

DIC N2 H

60x

Plan Apo

Oil

1.4

130

λ

100x

Plan Apo

Oil

1.45

130

λ

Special

  • Andor Revolution SD System (CSU-X)
  • Uniform spinning disk illumination
  • 2 cameras for ultra-fast dual color imaging
  • Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
  • Ultra-fast laser switching/ tuning (triggered acquisition)

Further information:

https://bua.openiris.io/Landing/Res...
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Possible user: No restrictions

Conditions of use:

The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

 

Note

New users – access upon training request here.

Email: ambio@charite.de

Provider:

Advanced Medical BIOimaging Core Facility

Provider Location:

Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany

Location:

Charité Campus Mitte
CCO Rm. 01-306
Virchowweg 6
10117 Berlin
Germany

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