Imaging modes
- Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
- Upright
Lasers and max. intensities out of 25x objective
- 405 nm : 6.3 mW
- 488 nm : 7.7 mW
- 561 nm : 9.7 mW
- 647 nm : 9.5 mW
Detectors
- Zyla 4.2 Plus sCMOS camera (2048×2048 pixels, 40 fps@16-bit, 53 fps@12-bit)
Emission Filters
|
Filter |
λ/Bandwidth |
|
Quad emission filter (DAPI, GFP, mCherry, Cy5) |
440/20 521/20 607/20 700/20 |
|
DAPI |
447/60 |
|
GFP |
525/25 |
|
mCherry |
600/52 |
|
Cy5 |
708/75 |
Objectives
|
Mag. |
Type |
Immer. |
NA |
WD [μm] |
Info |
|
10x |
Plan Achroat |
Water |
0.45 |
4,000 |
λ |
|
25x |
Apo |
water |
1.10 |
2,000 |
NCC |
Special
- Andor Revolution SD System (CSU-X1)
- Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
- Ultra-fast laser switching/ tuning (triggered acquisition)
Further information:
https://bua.openiris.io/Landing/Res...Possible user: No restrictions
Conditions of use:
https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2024-01-01.pdf
Note
New users – access upon training request here.
Email: ambio@charite.de
Provider:
Advanced Medical BIOimaging Core Facility
Provider Location:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany
Location:
Charité Campus Mitte
CCO Rm. 06-307
Virchowweg 6
10117 Berlin
Germany