Imaging modes
- Multiphoton confocal
- DIC, relief contrast
Laser (Mai Tai Insight Deepsee Dual)
- Tunable: 680 nm (600 mW) – 900 nm (500 mW) – 1300 nm (500 mW)
- Single line: 1040 nm
Emission Filters
|
Filter |
λ/Bandwidth |
|
DAPI |
– |
|
GFP |
525/50 |
|
mCherry |
595/50 |
|
Cy5 |
700/75 |
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 512×512 pixels, up to 420 fps in 512×32 pixels)
Non-Descanned Detectors (no pinhole)
- 2x GaAsP-NDD (highly sensitive, GFP, mCherry)
- 2x PMT-NDD (DAPI, Cy5)
Objectives
|
Mag. |
Type |
Immer. |
NA |
WD [μm] |
Info |
|
25x |
Apo |
Water |
1.1 |
2,000 |
NCC |
|
40x |
Apo |
Water |
0.8 |
3,500 |
NIR |
Special
- Upright
- Extended IR laser for deep tissue imaging
- Fast switch between glavo and resonant scanner
Further information:
https://bua.openiris.io/Landing/Res...Possible user: No restrictions
Conditions of use:
https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2024-01-01.pdf
Note
New users – access upon training request here.
Email: ambio@charite.de
Provider:
Advanced Medical BIOimaging Core Facility
Provider Location:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany
Location:
Charité Campus Mitte
CCO Rm. 02-117
Virchowweg 6
10117 Berlin
Germany