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Large-scale research equipment and laboratories / Imaging Systems

Multiphoton Confocal Microscope

17. AMBIO Nikon Multiphoton Confocal

Carrier: Charité

Usable by: HU | TU | FU | Charité

Imaging modes

  • Multiphoton confocal
  • DIC, relief contrast

Laser (Mai Tai Insight Deepsee Dual)

  • Tunable: 680 nm (600 mW) – 900 nm (500 mW) – 1300 nm (500 mW)
  • Single line: 1040 nm

Emission Filters

Filter

λ/Bandwidth

DAPI

–

GFP

525/50

mCherry

595/50

Cy5

700/75

Scanner 

  • Point scanner (galvo)
  • Resonant scanner (30 fps in 512×512 pixels, up to 420 fps in 512×32 pixels)

Non-Descanned Detectors (no pinhole)

  • 2x GaAsP-NDD (highly sensitive, GFP, mCherry)
  • 2x PMT-NDD (DAPI, Cy5)

Objectives

Mag.

Type

Immer.

NA

WD [μm]

Info

25x

Apo

Water

1.1

2,000

NCC

40x

Apo

Water

0.8

3,500

NIR

Special

  • Upright
  • Extended IR laser for deep tissue imaging
  • Fast switch between glavo and resonant scanner

Further information:

https://bua.openiris.io/Landing/Res...
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Possible user: No restrictions

Conditions of use:

The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

 

Note

New users – access upon training request here.

Email: ambio@charite.de

Provider:

Advanced Medical BIOimaging Core Facility

Provider Location:

Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany

Location:

Charité Campus Mitte
CCO Rm. 02-117
Virchowweg 6
10117 Berlin
Germany

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