Lattice Lightsheet Microscope

Imaging Modes

• Transmitted light with oblique contrast for sample positioning and overview
• Reflected light and epifluorescence with laser for beam adjustment and fluorescence light sheet imaging
• ibidi stage top incubation system (temperature, CO₂, humidity) and integrated auto-immersion mechanism enable unattended long-term experiments
• Photo-manipulation: 405 and 473 nm and lasers for FRAP and photo-activation/photo-switch and 780 nm laser for ablation experiments

Acquisition

• Speed: Volume: 3 Vol/s @300 μm x 50 μm x 20 μm, plane: 400 frames/s @300 μm x 20 μm
• Penetration depth up to 80 – 200 μm

Illumination

• LED (white & red) for transmitted light
• Laser for reflected light & epi-fluorescence: 488 nm (2 mW), 561 nm (2 mW), 640 nm (1 mW) 

Detectors

• pco.edge 4.2 CLHS sCMOS camera (2048×2048 pixels, 300 x 300 µm, 100 fps@16-bit full frame, 82% QE, 6.5 µm pixel size)

Software

• ZEN 3.5 (blue edition)
• Lattice Lightsheet Processing Module (deskewing, coverglass transformation)
• arivis Vision4D® software for advanced stitching, channel shift, high resolution volume rendering, and powerful deconvolution

Special

• Ultra-fast live 3D subcellular imaging with minimal phototoxicity and bleaching
• Near-isotropic resolution along the X, Y, and Z axes
• Using standard coverslips, 35 mm cell culture imaging dishes or well plates
• 5-axes stage tilt-able with high precision in X and Y
• Automatic water immersion dispenser

Objectives/Optics

• Illumination (light sheet): 13.3x / NA 0.4
• Detection: 44.83x / NA 1.0

Filters

Further information: