Abberior Facility Line STED
AMBIO-satellite at Charité Campus Benjamin Franklin (CBF) department of clinical physiology and nutritional medicine
Hindenburgdamm 30
12203 Berlin (Steglitz)
Main building, 5th floor, elevator 15, room 5505A
Maintenance, training and assistance is done by Prof. Dr. Dorothee Günzel and PD Dr. Jörg Piontek. Please contact us regarding labelling–especially if trying to push resolution limits (dorothee.guenzel@charite.de; joerg.piontek@charite.de).
MAIN FEATURE: High-end confocal and super resolution imaging platform:
- One STED laser (775 nm) and four excitation lasers (405, 485, 561, 640 nm)
- Patented adaptive illumination methods enable imaging with reduced photobleaching and phototoxicity
- Multi-channel, multi-position, time series, multi-ROI & tiling/stitching
- FRAP, FLIP, photoactivation, colocalization
Olympus IX83 inverted basis:
Specifications
Imaging Modes
STED plus confocal:
- Two main STED (stimulated emission depletion) -channels: StarRed/Atto647N/SiR (with excitation at 640 nm) & StarOrange/Alexa Fluor 594 (with excitation at 561 nm)
- An optional third less efficient STED channel, for instance for STAR 460L (long Stokes-shift dye) with excitation at 485 nm
- Plus up to two confocal channels: DAPI & Alexa Fluor 488/GFP (or similar fluorophores)
- 2D-STED: Resolution XY > 40 nm, Z > 500 nm including Z-stacks for 3D-imaging
- 3D-STED: Resolution down to XYZ > 100 nm including Z-stacks for 3D-imaging
Confocal only:
- Resolution down to XY > 220 nm, Z > 500 nm
- 4 channels with fluorophores similar to DAPI, Alexa Fluor 488/GFP, Alexa Fluor 555/Alexa Fluor 594 & Alexa Fluor 647/Cy5
Widefield EPI fluorescence:
- 4 channels with fluorophores similar to DAPI, Alexa Fluor 488/GFP, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647/Cy5
Excitation Lasers
STED-Laser
Detectors
Objectives
- 10x UPLSAPO 10X2 Air NA 0.4 WD 3100 µm
- 20x UPLFLN 20X Air NA 0.5 WD 2100 µm
- 20x UPLXAPO 20X Oil NA 0.8 WD 610 µm
- 60x UPLSAPO 60XW Water vNA 1.2 WD 280 µm
- 60x UPLXAPO 60XO Oil NA 1.42 WD 150 µm
Special
- Adaptive illumination via RESCue, DyMIN, and MINFIELD
- RAYSHAPE dynamic aberration correction
- EASY3D STED
- Confocal & STED autofocus
- Spectral RAINBOW detection
- Autoalignment
- Software Base Package Imspector for Facility Line
Further information about the Abberior Facility Line STED can be found at the product homepage or obtained from the AMBIO satellite at Charité – Universitätsmedizin Berlin, CBF, Med. Klinik f. Gastroenterologie, Infektiologie und Rheumatologie – Klinische Physiologie / Ernährungsmedizin (dorothee.guenzel@charite.de; joerg.piontek@charite.de).
Further information:
https://bua.openiris.io/Landing/Res...Possible user: No restrictions
Conditions of use:
The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.
Email: ambio@charite.de
Provider:
Advanced Medical BIOimaging Core Facility
Provider Location:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany
Location:
Charité Campus Benjamin Franklin
Department of clinical physiology and nutritional medicine
Hindenburgdamm 30
12203 Berlin (Steglitz)
Main building
5th floor
elevator 15
room 5505A