Zurück zur Übersicht

Shared Service

Forschungsgeräte und -labore / Bildgebung

Konfokales Mikroskop

11. AMBIO Nikon Scanning Confocal A1Rsi+

Betreiber: Charité

Nutzbar durch: HU | TU | FU | Charité

Imaging modes

  • Scanning confocal (DAPI, GFP, mCherry, Cy5)
  • FRAP/PA (photo activation/conversion)
  • Live-cell incubator (temperature, CO2, humidity)
  • Spectral scans
  • FLIM/FCS

 

Lasers and max. intensities out of 60x oil objective

  • 405 nm : 0.44 mW
  • 488 nm : 0.80 mW 
  • 561 nm : 1.66 mW 
  • 647 nm : 1.70 mW

 

Emission Filters

Filter

λ/Bandwidth

DAPI

450/50

GFP

525/50

mCherry

595/50

Cy5

700/75

Scanner

  • Point scanner (galvo)
  • Resonant scanner (30 fps in 512×512 pixels, up to 420 fps in 512×32 pixels)
  • Pinhole: hexagonal, tunable

 

Detectors

  • 2x Hybrid GaAsP PMT (high sensitivity, GFP, mCherry)
  • 2x PMT (Dapi, Cy5)
  • Transmission light detector (DIC, phase)
  • Spectral detector (optical diffraction grid and 32-channel multianodes)

 

Objectives

Mag.

Type

Immer.

NA

WD [μm]

Info

10x

Plan Achroat

Air

0.3

10,500

  

20x

Plan Apo

Air

0.8

1,000

DIC N2 VC

40x

Apo

Water

1.25

180

DIC N2 λ S

60x

Plan Apo

Water

1.27

170

DIC N2 IR

40x

Plan Flour

Oil

1.3

200

DIC N2 H

60x

Plan Apo

Oil

1.4

130

λ

 

Special

  • Fast switch between galvo and resonant scanners
  • Spectral scanner (unmixing)

Weiterführende Informationen:

https://bua.openiris.io/Landing/Res...
Alle einklappen

Mögliche Nutzer:innengruppen: No restrictions

Nutzungsvoraussetzungen:

The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

 

Hinweis

New users – access upon training request here.

E-Mail: ambio@charite.de

Anbieter:

Advanced Medical BIOimaging Core Facility

Standort Anbieter:

Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany

Standort:

Charité Campus Mitte
CCO Rm. 01-307
Virchowweg 6
10117 Berlin
Germany

Ähnliche Service-Angebote