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16. AMBIO Nikon TIRF/STORM

Betreiber: Charité

Nutzbar durch: HU | TU | FU | Charité

Imaging modes

  • Widefield EPI fluorescence (DAPI, GFP, mCherry, Cy5)
  • Phase (10x, 20x)
  • DIC (20x, 40x, 100x)
  • TIRF (adhesive cell membrane), motorized TIRF angle 
  • dSTORM (super-resolution, single molecule localization) 
  • FRAP/ PA (photo activation/ conversion)
  • Live-cell incubator (temperature, CO2, humidity)

Lasers (max. intensities out of 100x objective)

  • 405 nm : 3.31 mW
  • 488 nm : 53.5 mW 
  • 561 nm : 37.4 mW 
  • 647 nm : 80.7 mW

Emission filters

High speed emission filter wheel (OptoSpin IV) for clean multi-color imaging:

Filter

λ/Bandwidth

GFP

525/50

mCherry

600/37

Cy5

635 LP

Emission splitter (OptoSplit III) for simultaneous 2- or 3-color imaging:

 

SD-dSTORM cube

AF568, mCherry

AF647, CF680

 

Triple cube

GFP, AF488 (525/50)

AF568, mCherry (600/37)

AF647, CF680 (700/75)

Detectors

  • Camera 1 (left port): EMCCD, Andor iXon Ultra X3 987, 512×512 pixels, 16 µm pixel size, 56 fps full frame, QE max. 90%
  • Camera 2 (right port): sCMOS, Photometrics Prime 95B (95% QE, 1200×1200 pixels, 11 µm pixel size, 41 fps full frame, 82 fps@12-bit)
  • Camera 3 (left port front): CMOS, The Imaging Source DMK 23UX249 (1920×1200 pixels, 5.86 µm pixel size, 30 fps full frame)

Objectives

Mag.

Type

Immer.

NA

WD [μm]

Info

10x

Plan Flour

Air

0.3

15,200

Ph1 DL

40x

Plan Flour

Oil

1.3

240

DIC H N2

60x

Plan Apo

Oil

1.4

130

λ

100x

Plan Apo

Oil

1.5

120

DIC H N2 TIRF

(All objectives have an external phase contrast)

Special

  • 2x beam condenser for small FOV (dSTORM)
  • Ultra-fast laser switching/ tuning (triggered acquisition)

Weiterführende Informationen:

https://bua.openiris.io/Landing/Res...
Alle einklappen

Mögliche Nutzer:innengruppen: No restrictions

Nutzungsvoraussetzungen:

The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

 

Hinweis

New users – access upon training request here.

E-Mail: ambio@charite.de

Anbieter:

Advanced Medical BIOimaging Core Facility

Standort Anbieter:

Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany

Standort:

Charité Campus Mitte
CCO Rm. 01-306
Virchowweg 6
10117 Berlin
Germany

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