Imaging modes
- Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
- Upright
Lasers and max. intensities out of 25x objective
- 405 nm : 6.3 mW
- 488 nm : 7.7 mW
- 561 nm : 9.7 mW
- 647 nm : 9.5 mW
Detectors
- Zyla 4.2 Plus sCMOS camera (2048×2048 pixels, 40 fps@16-bit, 53 fps@12-bit)
Emission Filters
|
Filter |
λ/Bandwidth |
|
Quad emission filter (DAPI, GFP, mCherry, Cy5) |
440/20 521/20 607/20 700/20 |
|
DAPI |
447/60 |
|
GFP |
525/25 |
|
mCherry |
600/52 |
|
Cy5 |
708/75 |
Objectives
|
Mag. |
Type |
Immer. |
NA |
WD [μm] |
Info |
|
10x |
Plan Achroat |
Water |
0.45 |
4,000 |
λ |
|
25x |
Apo |
water |
1.10 |
2,000 |
NCC |
Special
- Andor Revolution SD System (CSU-X1)
- Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
- Ultra-fast laser switching/ tuning (triggered acquisition)
Weiterführende Informationen:
https://bua.openiris.io/Landing/Res...Mögliche Nutzer:innengruppen: No restrictions
Nutzungsvoraussetzungen:
The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.
Hinweis
New users – access upon training request here.
E-Mail: ambio@charite.de
Anbieter:
Advanced Medical BIOimaging Core Facility
Standort Anbieter:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany
Standort:
Charité Campus Mitte
CCO Rm. 06-307
Virchowweg 6
10117 Berlin
Germany