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Konfokales Mikroskop

14. AMBIO Nikon SoRa Spinning Disk Confocal CSU-W1

Betreiber: Charité

Nutzbar durch: HU | TU | FU | Charité

Imaging Modes

  • Spinning disk confocal (DAPI, CFP, GFP, YFP, mCherry, TxRED, Cy5)
  • SoRa-mode (Super Resolution by Optical Pixel Reassignment, 1.4x resolution improvement)
  • FRAP/ PA (photo activation/ conversion)
  • Widefield EPI fluorescence mode (DAPI, GFP, TxRED, TRITC-B, Cy5)
  • DIC for 10x, 40x, 60x, 100x objectives (upon request)
  • Live-cell incubator (temperature, CO2, humidity)

 7 Laser Lines

  • 405 nm
  • 445 nm
  • 488 nm
  • 515 nm
  • 561 nm
  • 594 nm
  • 638 nm 

 Detectors

  • 2x Hamamatsu ORCA-Fusion digital sCMOS cameras (2304×2304 pixels, 100 fps @ 2304×2048 pixels, 80% QE, 6.5 µm pixel size)
  • Dual-camera system for simultaneous dual-color detection (e.g. GFP/RFP, RFP/SiR, GFP/SiR, upon request)

 Software

  • NIS-Elements AR (Advanced Research) including modules for:
  • JOBS (automation)
  • Deconvolution
  • NIS.ai (Artificial Intelligence): denoise, clarify, enhance, segment, convert

 Special

  • Nipkow CSU-W for large FOV 1024×1024 px [size in µm for 10x]
  • Uniform spinning disk illumination
  • Enhanced signal-to-noise ratio compared to CSU-X
  • 2 cameras for simultaneous dual-color imaging
  • Fast emission filter wheel for clean multi-color imaging
  • Ultra-fast laser switching for triggered multicolor acquisition
  • SoRa-mode: Enhanced resolution (1.4x) in spinning disk mode
  • Automatic water immersion dispenser (WID)

Emission Filters

Filter λ/Bandwidth
Quadband (DAPI, GFP, mCherry, Cy5) 440/40
521/21
607/34
700/45
DAPI 447/60
CFP 472/30
GFP 525/50
YFP 542/27
mCherry 600/50
Cy5 685/40

Objectives

Mag. Type Immer. NA WD [μm] Info
10x (not installed) Plan Achroat Air 0.45 4,000 DIC N1 λ OFN25
40x Apo LWD Water 1.15 600 DIC N2 λS
60x Plan Apo Water 1.2 310-280 DIC N2 VC OFN25
60x Plan Apo Oil 1.4 130 DIC N2 λ OFN25
25x Plan Apo Silicone 1.05 550 DIC N2 λS OFN25
40x Plan Apo Silicone 1.25 300 DIC N2 λS OFN25
100x Plan Apo Silicone 1.35 300-290 SR HP DIC N2 λS OFN22

Weiterführende Informationen:

https://bua.openiris.io/Landing/Res...
Alle einklappen

Mögliche Nutzer:innengruppen: No restrictions

Nutzungsvoraussetzungen:

The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

 

Hinweis

New users – access upon training request here.

E-Mail: ambio@charite.de

Anbieter:

SupraFAB

Standort Anbieter:

Freie Universität Berlin

Standort:

SupraFAB
Altensteinstraße 23a
14195 Berlin
Room 101

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