Zurück zur Übersicht

Shared Service

Forschungsgeräte und -labore / Bildgebung

Weitwinkelmikroskop

10. AMBIO Nikon Widefield Ti2

Betreiber: Charité

Nutzbar durch: HU | TU | FU | Charité

General

Systems is equipped for multiplexed 3D live-cell experiments:

  • Multi-color, multi-position, time series, large image scan & automation
  • Fully motorized high precision stage for accurate repositioning (XYZ-control)
  • Stage incubator (temperature, CO2, humidity & carbogen)
    • Perfusion
    • Focus drift correction (Nikon PFS)
    • Water immersion objectives for high-resolution on water-based samples

      • Imaging Modes

      • Widefield epifluorescence (DAPI, CFP, GFP, YFP, mCherry & Cy5)
      • Bright-field (transmitted light) for histological slides (color staining)
      • Phase microscopy automated for 10x & 20x objectives
        • Interference Reflection Microscopy (IRM)
        • Polarized light microscopy
        • FRET

          • LED Illumination (Lumencore, SpectraX) and max. intensities out of 40x objective

          • 395 nm (395/25): 40 mW
          • 440 nm (438/29): 69 mW
          • 470 nm (475/28): 37 mW
          • 508 nm (511/16): 8 mW
          • 555 nm (555/28): 108 mW
          • 640 nm (635/22): 26 mW

            • Emission Filters

            Filter λ/Bandwidth
            Quad F67-401NXL (DAPI, GFP, mCherry, Cy5) 435/33
            520/26
            595/38
            695/63
            CFP ff01-482/35-32 482/35
            YFP ET540/30, F47-543 540/30
            GFP ET519/26M, F47-519NXL 519/26
            mCherry BrightLight 595/31 595/31
            Cy5 ET697/60M, F47-697NXL 697/60

            Detectors

            • For fluorescent samples: sCMOS, PCO.edge camera (>80% QE, 2048×2048 pixels, 6.5 µm pixel size, 53 fps full frame, up to 500 fps in small area)
            • For stained samples (histology): Nikon DS-Ri2 color CMOS camera (4908×3264 pixels, 6 fps full frame, 45 fps in 1636×1088 pixels)

              • Objectives

              Mag. Type Immer. NA WD [μm] Info
              4x PlanApo λ Air 0.2 20,000 OFN25, ∞/-
              10x PlanFluo Ph1 DL Air 0.3 15,200 OFN25, ∞/1.2
              20x PlanApo Ph2 DM Air 0.75 1,000 ∞/0.17
              40x Plan Apo λ DIC N2 Air 0.95 250-170 OFN25, ∞/0.11-0.23
              40x Apo LWD λS DIC N2 Water 1.15 590-610 ∞/0.15-0.19
              60x Plan Apo λ DIC N2 Oil 1.4 130 OFN25, ∞/0.17

              Special

              • Fast LED switching (triggered acquisition)
              • Fast emission filter wheel for clean multi-color imaging of DAPI, CFP, GFP, YFP, mCherry & Cy5
              • Automatic water immersion dispenser (WID) for water immersion objectives
              • NIS-Elements AR (Advanced Research) software including modules for: JOBS (automation), 3D Deconvolution & NIS.ai (artificial intelligence): denoise, clarify, enhance, segment & convert

              Weiterführende Informationen:

              https://bua.openiris.io/Landing/Res...
              Alle einklappen

              Mögliche Nutzer:innengruppen: No restrictions

              Nutzungsvoraussetzungen:

              The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
              including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
              understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
              steering committee. Registered Users and PIs will be notified of essential changes (service fees).
              Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
              as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

               

              Hinweis

              New users – access upon training request here.

              E-Mail: ambio@charite.de

              Anbieter:

              Advanced Medical BIOimaging Core Facility

              Standort Anbieter:

              Campus Charité Mitte (CCM)
              CharitéCrossOver (CCO)
              Rm 01-306
              Virchowweg 6
              10117 Berlin
              Germany

              Standort:

              Charité Campus Mitte
              CCO Rm. 01-307
              Virchowweg 6
              10117 Berlin
              Germany

              Ähnliche Service-Angebote