Imaging modes
- Scanning confocal (DAPI, GFP, mCherry, Cy5)
- FRAP/PA (photo activation/conversion)
- Live-cell incubator (temperature, CO2, humidity)
- Spectral scans
- FLIM/FCS
Lasers and max. intensities out of 60x oil objective
- 405 nm : 0.44 mW
- 488 nm : 0.80 mW
- 561 nm : 1.66 mW
- 647 nm : 1.70 mW
Emission Filters
|
Filter |
λ/Bandwidth |
|
DAPI |
450/50 |
|
GFP |
525/50 |
|
mCherry |
595/50 |
|
Cy5 |
700/75 |
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 512×512 pixels, up to 420 fps in 512×32 pixels)
- Pinhole: hexagonal, tunable
Detectors
- 2x Hybrid GaAsP PMT (high sensitivity, GFP, mCherry)
- 2x PMT (Dapi, Cy5)
- Transmission light detector (DIC, phase)
- Spectral detector (optical diffraction grid and 32-channel multianodes)
Objectives
|
Mag. |
Type |
Immer. |
NA |
WD [μm] |
Info |
|
10x |
Plan Achroat |
Air |
0.3 |
10,500 |
|
|
20x |
Plan Apo |
Air |
0.8 |
1,000 |
DIC N2 VC |
|
40x |
Apo |
Water |
1.25 |
180 |
DIC N2 λ S |
|
60x |
Plan Apo |
Water |
1.27 |
170 |
DIC N2 IR |
|
40x |
Plan Flour |
Oil |
1.3 |
200 |
DIC N2 H |
|
60x |
Plan Apo |
Oil |
1.4 |
130 |
λ |
Special
- Fast switch between galvo and resonant scanners
- Spectral scanner (unmixing)
Weiterführende Informationen:
https://bua.openiris.io/Landing/Res...Mögliche Nutzer:innengruppen: No restrictions
Nutzungsvoraussetzungen:
The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.
Hinweis
New users – access upon training request here.
E-Mail: ambio@charite.de
Anbieter:
Advanced Medical BIOimaging Core Facility
Standort Anbieter:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany
Standort:
Charité Campus Mitte
CCO Rm. 01-307
Virchowweg 6
10117 Berlin
Germany