Imaging modes
- Scanning confocal (DAPI, GFP, mCherry, Cy5)
- FRAP/PA (photo activation/conversion)
- Live-cell incubator (temperature, CO2, humidity)
- Spectral scans
- FLIM/FCS
Lasers and max. intensities out of 60x oil objective
- 405 nm : 0.44 mW
- 488 nm : 0.80 mW
- 561 nm : 1.66 mW
- 647 nm : 1.70 mW
Emission Filters
|
Filter |
λ/Bandwidth |
|
DAPI |
450/50 |
|
GFP |
525/50 |
|
mCherry |
595/50 |
|
Cy5 |
700/75 |
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 512×512 pixels, up to 420 fps in 512×32 pixels)
- Pinhole: hexagonal, tunable
Detectors
- 2x Hybrid GaAsP PMT (high sensitivity, GFP, mCherry)
- 2x PMT (Dapi, Cy5)
- Transmission light detector (DIC, phase)
- Spectral detector (optical diffraction grid and 32-channel multianodes)
Objectives
|
Mag. |
Type |
Immer. |
NA |
WD [μm] |
Info |
|
10x |
Plan Achroat |
Air |
0.3 |
10,500 |
|
|
20x |
Plan Apo |
Air |
0.8 |
1,000 |
DIC N2 VC |
|
40x |
Apo |
Water |
1.25 |
180 |
DIC N2 λ S |
|
60x |
Plan Apo |
Water |
1.27 |
170 |
DIC N2 IR |
|
40x |
Plan Flour |
Oil |
1.3 |
200 |
DIC N2 H |
|
60x |
Plan Apo |
Oil |
1.4 |
130 |
λ |
Special
- Fast switch between galvo and resonant scanners
- Spectral scanner (unmixing)
Mögliche Nutzer:innengruppen: No restrictions
Nutzungsvoraussetzungen: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2024-01-01.pdf
Hinweis
New users – access upon training request here.
E-Mail: ambio@charite.de
Standort:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany