Konfokales Mikroskop

AMBIO Zeiss Upright LSM 900 Airyscan 2

Compact confocal for gentle multiplex (multi-channel, XYZT-dimensions, automation) imaging:

4 laser lines
2x GaAsP detectors, 1x Airyscan2 detector, 1x transmitted light detector
Upright stage suited for fixed slides
Monochromatic fluorescence camera
Experiment-designer for optimal channel/ filter settings & acquisition speed
Product page: https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html

Imaging modes

Super-resolution & higher speed with Airyscan 2 (32x-array-detector for pixel re-assignment)
Joint deconvolution of Airyscan 2 images for higher resolution
Scanning confocal (DAPI, GFP, mCherry, Cy5, transmitted light)
Improved confocal image quality with LSM Plus (linear Wiener filter for enhanced signal to noise & improved resolution)
FRAP/PA (photo activation/conversion)
Multiplex SR-2Y (increased speed with parallel scanning of 2 lines)

Lasers

Objectives

2.5x Plan-Apochromat (air), NA=0.085, working distance (WD)=8800 µm
10x Plan-Apochromat (air), NA=0.45, WD=2100 µm
20x Plan-Apochromat (air), NA=0.8, WD=550 µm
25x LD LCI Plan-Apochromat (multi immersion: water, silicone, glycerol, oil), NA=0.8, WD=570 µm, DIC
40x LD LCI Plan-Apochromat (multi immersion: water, silicone, glycerol), NA=1.2, WD=410 µm, –> ONLY this objective is optimized for Airyscan mode!
63x Plan-Apochromat (oil), NA=1.4, WD=190 µm, DIC