Zurück zur Übersicht

Shared Service

Forschungsgeräte und -labore / Bildgebung

Konfokales Mikroskop

12. AMBIO Zeiss Upright LSM 900 Airyscan 2

Betreiber: Charité

Nutzbar durch: HU | TU | FU | Charité

AMBIO Zeiss Upright LSM 900 Airyscan 2

 

Compact confocal for gentle multiplex (multi-channel, XYZT-dimensions, automation) imaging:

  • 4 laser lines
  • 2x GaAsP detectors, 1x Airyscan2 detector, 1x transmitted light detector
  • Upright stage suited for fixed slides
  • Monochromatic fluorescence camera
  • Experiment-designer for optimal channel/ filter settings & acquisition speed
  • Product page: https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html

 

Imaging modes

  • Super-resolution & higher speed with Airyscan 2 (32x-array-detector for pixel re-assignment)
  • Joint deconvolution of Airyscan 2 images for higher resolution
  • Scanning confocal (DAPI, GFP, mCherry, Cy5, transmitted light)
  • Improved confocal image quality with LSM Plus (linear Wiener filter for enhanced signal to noise & improved resolution)
  • FRAP/PA (photo activation/conversion)
  • Multiplex SR-2Y (increased speed with parallel scanning of 2 lines)

Lasers

  • 385 nm
  • 475 nm
  • 555 nm
  • 630 nm

 

Objectives

  • 2.5x Plan-Apochromat (air), NA=0.085, working distance (WD)=8800 µm
  • 10x Plan-Apochromat (air), NA=0.45, WD=2100 µm
  • 20x Plan-Apochromat (air), NA=0.8, WD=550 µm
  • 25x LD LCI Plan-Apochromat (multi immersion: water, silicone, glycerol, oil), NA=0.8, WD=570 µm, DIC 
  • 40x LD LCI Plan-Apochromat (multi immersion: water, silicone, glycerol), NA=1.2, WD=410 µm, –> ONLY this objective is optimized for Airyscan mode! 
  • 63x Plan-Apochromat (oil), NA=1.4, WD=190 µm, DIC

Weiterführende Informationen:

https://bua.openiris.io/Landing/Res...
Alle einklappen

Mögliche Nutzer:innengruppen: No restrictions

Nutzungsvoraussetzungen:

The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

 

Hinweis

Access upon request of analysis project here.

E-Mail: ambio@charite.de

Anbieter:

Advanced Medical BIOimaging Core Facility

Standort Anbieter:

Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany

Standort:

Charité Campus Mitte
CCO Rm. 05-311
Virchowweg 6
10117 Berlin
Germany

Ähnliche Service-Angebote