Imaging modes
- Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
- FRAP/ PA (photo activation/ conversion)
- Live-cell incubator (temperature, CO2, humidity)
Lasers and max. intensities out of 60x oil objective
- 405 nm : 3.54 mW
- 488 nm : 3.85 mW
- 561 nm : 4.11 mW
- 647 nm : 4.68 mW
Emission Filters
|
Filter |
λ/Bandwidth |
|
Quad emission filter (DAPI, GFP, mCherry, Cy5) |
440/40 521/21 607/34 700/45 |
|
DAPI |
452/45 |
|
GFP |
525/50 |
|
mCherry |
600/50 |
|
Cy5 |
685/40 |
|
GFP narrow |
510/20 |
Detectors
- 2 EMCCD cameras (iXon3 DU-888 Ultra, 1024×1024 pixels, 13 μm pixel size, 26 fps full frame, usable area ~512×512 pixels, 93 fps)
- Current dual color dichroic for simultaneous GFP & mCherry detection
Objectives
|
Mag. |
Type |
Immer. |
NA |
WD [μm] |
Info |
|
10x (not installed) |
Plan Achroat |
Air |
0.3 |
10,500 |
|
|
20x |
Plan Apo |
Air |
0.8 |
1,000 |
DIC N2 VC |
|
40x |
Apo |
Water |
1.25 |
180 |
DIC N2 λ S |
|
60x |
Plan Apo |
Water |
1.27 |
170 |
DIC N2 IR |
|
40x |
Plan Flour |
Oil |
1.3 |
200 |
DIC N2 H |
|
60x |
Plan Apo |
Oil |
1.4 |
130 |
λ |
|
100x |
Plan Apo |
Oil |
1.45 |
130 |
λ |
Special
- Andor Revolution SD System (CSU-X)
- Uniform spinning disk illumination
- 2 cameras for ultra-fast dual color imaging
- Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
- Ultra-fast laser switching/ tuning (triggered acquisition)
Weiterführende Informationen:
https://bua.openiris.io/Landing/Res...Mögliche Nutzer:innengruppen: No restrictions
Nutzungsvoraussetzungen:
The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.
Hinweis
New users – access upon training request here.
E-Mail: ambio@charite.de
Anbieter:
Advanced Medical BIOimaging Core Facility
Standort Anbieter:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany
Standort:
Charité Campus Mitte
CCO Rm. 01-306
Virchowweg 6
10117 Berlin
Germany