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Konfokales Mikroskop

14. AMBIO Nikon SoRa Spinning Disk Confocal CSU-W1

Betreiber: Charité

Nutzbar durch: HU | TU | FU | Charité

Imaging Modes

  • Spinning disk confocal (DAPI, CFP, GFP, YFP, mCherry, TxRED, Cy5)
  • SoRa-mode (Super Resolution by Optical Pixel Reassignment, 1.4x resolution improvement)
  • FRAP/ PA (photo activation/ conversion)
  • Widefield EPI fluorescence mode (DAPI, GFP, TxRED, TRITC-B, Cy5)
  • DIC for 10x, 40x, 60x, 100x objectives (upon request)
  • Live-cell incubator (temperature, CO2, humidity)

 7 Laser Lines

  • 405 nm
  • 445 nm
  • 488 nm
  • 515 nm
  • 561 nm
  • 594 nm
  • 638 nm 

 Detectors

  • 2x Hamamatsu ORCA-Fusion digital sCMOS cameras (2304×2304 pixels, 100 fps @ 2304×2048 pixels, 80% QE, 6.5 µm pixel size)
  • Dual-camera system for simultaneous dual-color detection (e.g. GFP/RFP, RFP/SiR, GFP/SiR, upon request)

 Software

  • NIS-Elements AR (Advanced Research) including modules for:
  • JOBS (automation)
  • Deconvolution
  • NIS.ai (Artificial Intelligence): denoise, clarify, enhance, segment, convert

 Special

  • Nipkow CSU-W for large FOV 1024×1024 px [size in µm for 10x]
  • Uniform spinning disk illumination
  • Enhanced signal-to-noise ratio compared to CSU-X
  • 2 cameras for simultaneous dual-color imaging
  • Fast emission filter wheel for clean multi-color imaging
  • Ultra-fast laser switching for triggered multicolor acquisition
  • SoRa-mode: Enhanced resolution (1.4x) in spinning disk mode
  • Automatic water immersion dispenser (WID)

Emission Filters

Filter λ/Bandwidth
Quadband (DAPI, GFP, mCherry, Cy5) 440/40
521/21
607/34
700/45
DAPI 447/60
CFP 472/30
GFP 525/50
YFP 542/27
mCherry 600/50
Cy5 685/40

Objectives

Mag. Type Immer. NA WD [μm] Info
10x (not installed) Plan Achroat Air 0.45 4,000 DIC N1 λ OFN25
40x Apo LWD Water 1.15 600 DIC N2 λS
60x Plan Apo Water 1.2 310-280 DIC N2 VC OFN25
60x Plan Apo Oil 1.4 130 DIC N2 λ OFN25
25x Plan Apo Silicone 1.05 550 DIC N2 λS OFN25
40x Plan Apo Silicone 1.25 300 DIC N2 λS OFN25
100x Plan Apo Silicone 1.35 300-290 SR HP DIC N2 λS OFN22

Weiterführende Informationen:

https://bua.openiris.io/Landing/Res...
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Mögliche Nutzer:innengruppen: No restrictions

Nutzungsvoraussetzungen:

https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2024-01-01.pdf

Hinweis

New users – access upon training request here.

E-Mail: ambio@charite.de

Anbieter:

SupraFAB

Standort Anbieter:

Freie Universität Berlin

Standort:

SupraFAB
Altensteinstraße 23a
14195 Berlin
Room 101

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