Imaging modes
- Widefield EPI fluorescence (DAPI, GFP, mCherry, Cy5)
- Phase (10x, 20x)
- DIC (20x, 40x, 100x)
- TIRF (adhesive cell membrane), motorized TIRF angle
- dSTORM (super-resolution, single molecule localization)
- FRAP/ PA (photo activation/ conversion)
- Live-cell incubator (temperature, CO2, humidity)
Lasers (max. intensities out of 100x objective)
- 405 nm : 3.31 mW
- 488 nm : 53.5 mW
- 561 nm : 37.4 mW
- 647 nm : 80.7 mW
Emission filters
High speed emission filter wheel (OptoSpin IV) for clean multi-color imaging:
|
Filter |
λ/Bandwidth |
|
GFP |
525/50 |
|
mCherry |
600/37 |
|
Cy5 |
635 LP |
Emission splitter (OptoSplit III) for simultaneous 2- or 3-color imaging:
|
SD-dSTORM cube |
|
|
AF568, mCherry |
|
|
AF647, CF680 |
|
|
Triple cube |
|
|
GFP, AF488 (525/50) |
|
|
AF568, mCherry (600/37) |
|
|
AF647, CF680 (700/75) |
Detectors
- Camera 1 (left port): EMCCD, Andor iXon Ultra X3 987, 512×512 pixels, 16 µm pixel size, 56 fps full frame, QE max. 90%
- Camera 2 (right port): sCMOS, Photometrics Prime 95B (95% QE, 1200×1200 pixels, 11 µm pixel size, 41 fps full frame, 82 fps@12-bit)
- Camera 3 (left port front): CMOS, The Imaging Source DMK 23UX249 (1920×1200 pixels, 5.86 µm pixel size, 30 fps full frame)
Objectives
|
Mag. |
Type |
Immer. |
NA |
WD [μm] |
Info |
|
10x |
Plan Flour |
Air |
0.3 |
15,200 |
Ph1 DL |
|
40x |
Plan Flour |
Oil |
1.3 |
240 |
DIC H N2 |
|
60x |
Plan Apo |
Oil |
1.4 |
130 |
λ |
|
100x |
Plan Apo |
Oil |
1.5 |
120 |
DIC H N2 TIRF |
(All objectives have an external phase contrast)
Special
- 2x beam condenser for small FOV (dSTORM)
- Ultra-fast laser switching/ tuning (triggered acquisition)
Weiterführende Informationen:
https://bua.openiris.io/Landing/Res...Mögliche Nutzer:innengruppen: No restrictions
Nutzungsvoraussetzungen:
https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2024-01-01.pdf
Hinweis
New users – access upon training request here.
E-Mail: ambio@charite.de
Anbieter:
Advanced Medical BIOimaging Core Facility
Standort Anbieter:
Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany
Standort:
Charité Campus Mitte
CCO Rm. 01-306
Virchowweg 6
10117 Berlin
Germany