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Gitter-Lichtschnitt-Mikroskop

18. AMBIO ZEISS Lattice Lightsheet 7

Betreiber: Charité

Nutzbar durch: HU | TU | FU | Charité

Imaging Modes

  • Transmitted light with oblique contrast for sample positioning and overview
  • Reflected light and epifluorescence with laser for beam adjustment and fluorescence light sheet imaging
  • ibidi stage top incubation system (temperature, CO2, humidity) and integrated auto-immersion mechanism enable unattended long-term experiments
  • Photo-manipulation: 405 and 473 nm and lasers for FRAP and photo-activation/photo-switch and 780 nm laser for ablation experiments

Acquisition

  • Speed: Volume: 3 Vol/s @300 μm x 50 μm x 20 μm, plane: 400 frames/s @300 μm x 20 μm
  • Penetration depth up to 80 – 200 μm

Illumination

  • LED (white & red) for transmitted light
  • Laser for reflected light & epi-fluorescence: 488 nm (2 mW), 561 nm (2 mW), 640 nm (1 mW) 

Detectors

  • pco.edge 4.2 CLHS sCMOS camera (2048×2048 pixels, 300 x 300 µm, 100 fps@16-bit full frame, 82% QE, 6.5 µm pixel size)

Software

  • ZEN 3.5 (blue edition)
  • Lattice Lightsheet Processing Module (deskewing, coverglass transformation)
  • arivis Vision4D® software for advanced stitching, channel shift, high resolution volume rendering, and powerful deconvolution

Special

  • Ultra-fast live 3D subcellular imaging with minimal phototoxicity and bleaching
  • Near-isotropic resolution along the X, Y, and Z axes
  • Using standard coverslips, 35 mm cell culture imaging dishes or well plates
  • 5-axes stage tilt-able with high precision in X and Y
  • Automatic water immersion dispenser

Objectives/Optics

  • Illumination (light sheet): 13.3x / NA 0.4
  • Detection: 44.83x / NA 1.0

Filters

Filter (λ, Bandwidth) Sample Fluorophore
LBF 405/ 488/ 561/ 642 All, blocks excitation laser
BP 495-550/ BP 570-620 GFP, mCherry
BP 495-550 +LP655 GFP, Cy5
BP 570-620 +LP655 mCherry, Cy5
BP 495-590 GFP, YFP, mCherry
EF LP 570 mCherry, Cy5

Weiterführende Informationen:

https://bua.openiris.io/Landing/Res...
Alle einklappen

Mögliche Nutzer:innengruppen: No restrictions

Nutzungsvoraussetzungen:

The User and PI hereby declare to have understood and accepted the AMBIO User Guidelines and Rules
including the Safety Rules, as available online at: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User-Guidelines-Rules_2025-01-30.pdf. It is further
understood that these Guidelines and Rules may be updated or changed in agreement with the AMBIO
steering committee. Registered Users and PIs will be notified of essential changes (service fees).
Furthermore, the new User and PI have understood that a fee will be charged for the usage of AMBIO services
as outlined in the AMBIO User Guidelines and Rules, and that they are willing and able to pay the fees.

 

Hinweis

New users – access upon training request here.

E-Mail: ambio@charite.de

Anbieter:

Advanced Medical BIOimaging Core Facility

Standort Anbieter:

Campus Charité Mitte (CCM)
CharitéCrossOver (CCO)
Rm 01-306
Virchowweg 6
10117 Berlin
Germany

Standort:

Charité Campus Mitte
CCO Rm. 01-307
Virchowweg 6
10117 Berlin
Germany

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