OpenIRIS Resources
Name: CantoII "Thalia"
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description: Instrument configuration (download)
Contacts: cytometry-core@bih-charite.de
Comments:
You need to be registered and trained to use this instrument.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: CyTOF2/Helios "Tangerine"
Resource type: Mass Cytometer
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
Flow Cytometry and Mass Spectrometry fell in love and a new technology was born: Mass Cytometry, the best of both worlds.
As in conventional flow cytometry, cells within a
heterogeneous sample are analysed on the single cell level for a
multitude of parameters. However, instead of using fluorochrome-labelled
antibodies to bind to the antigen of interest and being limited to the
number of spectrally resolvable fluorochromes, mass cytometry uses metal
isotope-labelled antibodies that are analysed by their time-of-flight
(TOF). This allows for the analysis of currently >40 parameters per
cell and a deep profiling of phenotype and function of cells.
funded by DFG: INST 335/453-1 FUGG
Contacts: desiree.kunkel@bih-charite.de
Comments:
Usage of mass cytometry can be requested in "services".
- Please inform us about any changes in the acqusition template before you bring the samples.
Please contact the core facility at least 6 weeks before starting new projects to discuss technical details and instrument availability.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: Mass Cytometry (CyTOF) - Sample Acquisition
Resource type: Mass Cytometry
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description: Flow Cytometry and Mass Spectrometry fell in love and a new technology was born: Mass Cytometry, the best of both worlds.
As in conventional flow cytometry, cells within a heterogeneous sample are analysed on the single cell level for a multitude of parameters. However, instead of using fluorochrome-labelled antibodies to bind to the antigen of interest and being limited to the number of spectrally resolvable fluorochromes, mass cytometry uses metal isotope-labelled antibodies that are analysed by their time-of-flight (TOF). This allows for the analysis of currently >40 parameters per cell and a deep profiling of phenotype and function of cells.
Contacts: desiree.kunkel@bih-charite.de
Comments: Please request a project meeting at least 6 weeks before start of a new project to get access.
You can check CyTOF2/Helios availability before submitting a request, but be aware that not every available slot will fit for your specific measurement.
Operating hours: tue-thu 10:00-20:30, fr 10:00-17:30
Sample Acquisition Template: download
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: MRT1 - Siemens Prisma 3T
Resource type: Magnetic Resonance Imaging System
Provider: BCAN (Berlin Center for Advanced Neuroimaging)
Affiliations: Charité
Description: The Magnetom Trio is Powered by the Tim Technology that allows you to achieve the highest level of image quality for your patients. With this MRI system, you are able to streamline your planning and processing and be able to diagnose your patient’s most challenging diseases very quickly with confidence. This system delivers high gradient performance that is needed for the most demanding MRI applications.
Important documents:
- measurement protocol / Messprotokol
- visitor screening form / Fragebogen für Besucher
- information sheet for healthy subjects / Informationsblatt für gesunde Probanden
- form regarding the handing over of subject data at BCAN / Formular zur Aushändigung von Probandendaten am BCAN
Contacts: bcan-reservations@charite.de
Comments: Access to the MRI machine is only granted upon registration of a study here (after login) or here. If the study you are working for is already registered with us, you can ask the responsible person to be added as a participant.
Bookings have to be approved by the facility (indicated by '-'). You will receive an email once your booking is approved.
Provider UsagePolicy:
Nutzer:innen Studienleiter:innen verpflichten sich, folgende Regeln einzuhalten:
Alle Vorschriften der aktuellen Version dieser Messordnung sind beim Betrieb der MRTs am BCAN einzuhalten. Die Anleitungen und Anweisungen im Siemens-Betriebshandbuch des MRT sind zu beachten.
Das MRT darf nur in Anwesenheit von zwei durch das BCAN zertifizierten Nutzern betrieben werden, von denen einer Advanced User sein muss und der andere Advanced User oder Basic User sein kann.
Basic User sind berechtigt zur Begleitung eines Advanced Users, dürfen aber nicht selbständig messen. Die Advanced User und Basic User führen die Messungen im Auftrag ihres Studienleiters durch.
Ein Advanced User übernimmt zu Beginn einer Messung die Verantwortung für die Einhaltung aller Nutzerregeln. Sollten mehrere Advanced User an der Messung beteiligt sein, ist vor Beginn der Messung die für die Messung verantwortliche Person festzulegen.
Vor Beginn jeder Messung müssen alle Nutzer das Logbuch lesen, um über Betriebsänderungen auf dem Laufenden gehalten zu werden. Abweichungen vom Standard-Setup und andere Auffälligkeiten sind in das Logbuch einzutragen.
Der Gesundheit von Probanden und Patienten ist absolute Priorität einzuräumen.
Probanden und Patienten sind vor der Messung durch den Advanced User bzw. einen approbierten Arzt über die Gefahren der anstehenden Messung aufzuklären. Dazu ist eine schriftliche Einverständniserklärung einzuholen, die durch den Studienleiter zu archivieren ist. Probanden und Patienten sind vor der Studienteilnahme über die möglichen Konsequenzen von Zufallsbefunden aufzuklären.
Der Studienleiter hat zu gewährleisten, dass die Kommunikation zwischen Advanced User und Proband nicht durch sprachliche Hindernisse beeinträchtigt wird (z.B. bei ausländischen Probanden oder Advanced Usern).
Patienten, Probanden und alle beteiligten Mitarbeiter sind vor dem Betreten der HF-Kabine einem sorgfältigen Metall-Check zu unterziehen, wie im Aufklärungsbogen ausgeführt. Die Einhaltung der MRT-Ausschlusskriterien des BCAN sowie zusätzliche Kriterien der Ethikkommission ist durch den Advanced User zu gewährleisten.
Der Basic User und Advanced User Status muss einmal pro Kalenderjahr erneuert werden, ansonsten erlischt er.
Die MRTs und die zugehörige Technik sind in einwandfreiem Zustand zu halten und Störungen und Defekte sofort den Betreibern zu melden.
Es dürfen weder magnetische oder magnetisierbare Gegenstände noch irgendwelche technischen Geräte ohne Rücksprache mit der MR-Physik in die HF-Kabine mitgenommen werden.
Die Messung ist sorgfältig im fortlaufenden Protokollblatt zu dokumentieren, inklusive aller Auffälligkeiten, Störungen und Gerätedefekte.
Die erhobenen Daten sind sorgfältig und vollständig zu archivieren, und die Datenschutzvorschriften der Charité für medizinische Daten sind einzuhalten. Insbesondere sind alle erforderlichen Maßnahmen zu ergreifen, um die Weitergabe der Daten an Unbefugte zu verhindern.
Nur solche Probanden dürfen gescannt werden, die (1) krankenversichert sind und die (2) sich schriftlich bereit erklären, sich über Zufallsbefunde informieren zu lassen. Probanden sind darüber aufzuklären, dass bei Zufallsbefunden ein radiologischer Facharzt innerhalb der Charité zur Beurteilung der Daten hinzugezogen werden kann. Eine Abklärung von Zufallsbefunden ist zügig durchzuführen. Das Vorgehen ist mit der Leitung des BCAN abzustimmen. Probanden sind vor der Messung über nachteilige Konsequenzen von Zufallsbefunden (z.B. bei privater Krankenversichung oder Berufsunfähigkeitsversicherung) aufzuklären.
Bei Patientenstudien ist ein verantwortlicher Arzt einzubeziehen. Die erhobenen MRT-Daten von Patienten sind der neuroradiologischen Befundung zukommen zu lassen.
Die Studienleiter erhalten Schlüssel/Transponder-Zugang zu MRT-Räumen und MRT-Kabine. Die Studienleiter haben Sorge zu tragen, dass keine Unbefugten zu den MRT-Räumen Zugang haben. Zugang ist nur erlaubt für den Advanced User selbst, für an der Studie beteiligte Mitarbeiter, die selbst alle Basic User Status haben müssen, und für die zu messenden Probanden und Patienten. Besucher müssen vor dem Betreten der MRT-Räume einen Besucheraufklärungsbogen ausfüllen und sie müssen durch den Advanced User eine MRT-Gefahrenaufklärung bekommen. Sie dürfen die MRT-Kabine nicht betreten.
Bei Auslaufen des Advanced User Status muss der Nutzer alle Schlüssel für das BCAN an das BCAN-Sekretariat unaufgefordert zurückgeben.
Minderjährige Probanden dürfen nur nach vorheriger Rücksprache mit den Betreibern gescannt werden. Dabei ist das positive Ethikvotum vorzulegen. Der Versuchsleiter hat dafür Sorge zu tragen, dass alle ethischen juristischen, technischen und versicherungsrechtlichen Besonderheiten für Studien mit Minderjährigen berücksichtigt werden.
Gebuchte Messzeit muss voll bezahlt werden, es sei denn, sie wird mindestens 48 Stunden vorher storniert. Für die 48 Stunden sind nur Werktage (ohne Samstag) anzurechnen. Die Arbeitsgruppen bemühen sich im Interesse aller Nutzer, nicht benötigte Messzeit möglichst frühzeitig abzusagen.
Personen, die unter Einfluss von Alkohol oder Drogen sowie Personen, deren Fähigkeit zum Bedienen von Maschinen durch Medikamente beeinträchtigt ist (z.B. Psychopharmaka, Schmerzmittel) dürfen die MRT-Geräte am BCAN nicht bedienen (weder als Advanced noch als Basic User).
Schwangere dürfen die HF-Kabine nicht betreten (dies gilt für Besucher, Probanden, Patienten und Advanced und Basic User).
Bei Publikationen, in die Messungen oder andere Dienstleistungen des BCAN einfließen, ist das BCAN im Abschnitt „Acknowledgements“ anzugeben: „MR-imaging for this study was performed at the Berlin Center for Advanced Neuroimaging (BCAN)“.
Provider Location: Campus Charité Mitte (CCM), Berlin
Name: MRT2 - Siemens Prisma 3T
Resource type: Magnetic Resonance Imaging System
Provider: BCAN (Berlin Center for Advanced Neuroimaging)
Affiliations: Charité
Description: The Magnetom Trio is Powered by the Tim Technology that allows you to
achieve the highest level of image quality for your patients. With this
MRI system, you are able to streamline your planning and processing and
be able to diagnose your patient’s most challenging diseases very
quickly with confidence. This system delivers high gradient performance
that is needed for the most demanding MRI applications.
Important documents:
- measurement protocol / Messprotokol
- visitor screening form / Fragebogen für Besucher
- information sheet for healthy subjects / Informationsblatt für gesunde Probanden
- form regarding the handing over of subject data at BCAN / Formular zur Aushändigung von Probandendaten am BCAN
Contacts: bcan-reservations@charite.de
Comments: Access to the MRI machine is only granted upon registration of a study here (after login) or here. If the study you are working for is already registered with us, you can ask the responsible person to be added as a participant.
Bookings have to be approved by the facility (indicated by '-'). You will receive an email once your booking is approved.
Provider UsagePolicy:
Nutzer:innen Studienleiter:innen verpflichten sich, folgende Regeln einzuhalten:
Alle Vorschriften der aktuellen Version dieser Messordnung sind beim Betrieb der MRTs am BCAN einzuhalten. Die Anleitungen und Anweisungen im Siemens-Betriebshandbuch des MRT sind zu beachten.
Das MRT darf nur in Anwesenheit von zwei durch das BCAN zertifizierten Nutzern betrieben werden, von denen einer Advanced User sein muss und der andere Advanced User oder Basic User sein kann.
Basic User sind berechtigt zur Begleitung eines Advanced Users, dürfen aber nicht selbständig messen. Die Advanced User und Basic User führen die Messungen im Auftrag ihres Studienleiters durch.
Ein Advanced User übernimmt zu Beginn einer Messung die Verantwortung für die Einhaltung aller Nutzerregeln. Sollten mehrere Advanced User an der Messung beteiligt sein, ist vor Beginn der Messung die für die Messung verantwortliche Person festzulegen.
Vor Beginn jeder Messung müssen alle Nutzer das Logbuch lesen, um über Betriebsänderungen auf dem Laufenden gehalten zu werden. Abweichungen vom Standard-Setup und andere Auffälligkeiten sind in das Logbuch einzutragen.
Der Gesundheit von Probanden und Patienten ist absolute Priorität einzuräumen.
Probanden und Patienten sind vor der Messung durch den Advanced User bzw. einen approbierten Arzt über die Gefahren der anstehenden Messung aufzuklären. Dazu ist eine schriftliche Einverständniserklärung einzuholen, die durch den Studienleiter zu archivieren ist. Probanden und Patienten sind vor der Studienteilnahme über die möglichen Konsequenzen von Zufallsbefunden aufzuklären.
Der Studienleiter hat zu gewährleisten, dass die Kommunikation zwischen Advanced User und Proband nicht durch sprachliche Hindernisse beeinträchtigt wird (z.B. bei ausländischen Probanden oder Advanced Usern).
Patienten, Probanden und alle beteiligten Mitarbeiter sind vor dem Betreten der HF-Kabine einem sorgfältigen Metall-Check zu unterziehen, wie im Aufklärungsbogen ausgeführt. Die Einhaltung der MRT-Ausschlusskriterien des BCAN sowie zusätzliche Kriterien der Ethikkommission ist durch den Advanced User zu gewährleisten.
Der Basic User und Advanced User Status muss einmal pro Kalenderjahr erneuert werden, ansonsten erlischt er.
Die MRTs und die zugehörige Technik sind in einwandfreiem Zustand zu halten und Störungen und Defekte sofort den Betreibern zu melden.
Es dürfen weder magnetische oder magnetisierbare Gegenstände noch irgendwelche technischen Geräte ohne Rücksprache mit der MR-Physik in die HF-Kabine mitgenommen werden.
Die Messung ist sorgfältig im fortlaufenden Protokollblatt zu dokumentieren, inklusive aller Auffälligkeiten, Störungen und Gerätedefekte.
Die erhobenen Daten sind sorgfältig und vollständig zu archivieren, und die Datenschutzvorschriften der Charité für medizinische Daten sind einzuhalten. Insbesondere sind alle erforderlichen Maßnahmen zu ergreifen, um die Weitergabe der Daten an Unbefugte zu verhindern.
Nur solche Probanden dürfen gescannt werden, die (1) krankenversichert sind und die (2) sich schriftlich bereit erklären, sich über Zufallsbefunde informieren zu lassen. Probanden sind darüber aufzuklären, dass bei Zufallsbefunden ein radiologischer Facharzt innerhalb der Charité zur Beurteilung der Daten hinzugezogen werden kann. Eine Abklärung von Zufallsbefunden ist zügig durchzuführen. Das Vorgehen ist mit der Leitung des BCAN abzustimmen. Probanden sind vor der Messung über nachteilige Konsequenzen von Zufallsbefunden (z.B. bei privater Krankenversichung oder Berufsunfähigkeitsversicherung) aufzuklären.
Bei Patientenstudien ist ein verantwortlicher Arzt einzubeziehen. Die erhobenen MRT-Daten von Patienten sind der neuroradiologischen Befundung zukommen zu lassen.
Die Studienleiter erhalten Schlüssel/Transponder-Zugang zu MRT-Räumen und MRT-Kabine. Die Studienleiter haben Sorge zu tragen, dass keine Unbefugten zu den MRT-Räumen Zugang haben. Zugang ist nur erlaubt für den Advanced User selbst, für an der Studie beteiligte Mitarbeiter, die selbst alle Basic User Status haben müssen, und für die zu messenden Probanden und Patienten. Besucher müssen vor dem Betreten der MRT-Räume einen Besucheraufklärungsbogen ausfüllen und sie müssen durch den Advanced User eine MRT-Gefahrenaufklärung bekommen. Sie dürfen die MRT-Kabine nicht betreten.
Bei Auslaufen des Advanced User Status muss der Nutzer alle Schlüssel für das BCAN an das BCAN-Sekretariat unaufgefordert zurückgeben.
Minderjährige Probanden dürfen nur nach vorheriger Rücksprache mit den Betreibern gescannt werden. Dabei ist das positive Ethikvotum vorzulegen. Der Versuchsleiter hat dafür Sorge zu tragen, dass alle ethischen juristischen, technischen und versicherungsrechtlichen Besonderheiten für Studien mit Minderjährigen berücksichtigt werden.
Gebuchte Messzeit muss voll bezahlt werden, es sei denn, sie wird mindestens 48 Stunden vorher storniert. Für die 48 Stunden sind nur Werktage (ohne Samstag) anzurechnen. Die Arbeitsgruppen bemühen sich im Interesse aller Nutzer, nicht benötigte Messzeit möglichst frühzeitig abzusagen.
Personen, die unter Einfluss von Alkohol oder Drogen sowie Personen, deren Fähigkeit zum Bedienen von Maschinen durch Medikamente beeinträchtigt ist (z.B. Psychopharmaka, Schmerzmittel) dürfen die MRT-Geräte am BCAN nicht bedienen (weder als Advanced noch als Basic User).
Schwangere dürfen die HF-Kabine nicht betreten (dies gilt für Besucher, Probanden, Patienten und Advanced und Basic User).
Bei Publikationen, in die Messungen oder andere Dienstleistungen des BCAN einfließen, ist das BCAN im Abschnitt „Acknowledgements“ anzugeben: „MR-imaging for this study was performed at the Berlin Center for Advanced Neuroimaging (BCAN)“.
Provider Location: Campus Charité Mitte (CCM), Berlin
Name: Paraffin embedding center
Resource type: Embedding Station
Provider: iPATH.Berlin
Affiliations: Charité
Description:
All modules of the embedding station operate at the same working height and are arranged according to lab requirements. The system with separate temperature control elements consists of a dispensing element, a preheating unit and a separate cooling plate. We provide moulds and forceps.
Contacts: anja.kuehl@charite.de
Comments:
Please contact us via email (see contacts) if you are interested in using the instrument. You will receive a short introduction prior to getting access to the instrument.
Booking slot duration: 30 - 90 min
Free cancellation of bookings at least 24 hours before start.
Provider UsagePolicy:
Nutzerordnung
Gültig ab 01.Januar 2021
Präambel
Die zentrale Forschungseinrichtung iPATH.Berlin – Immunpathologie für Experimentelle Modelle ist eine translationale Einrichtung als Teil der Forschungsinfrastruktur der Charité – Universitätsmedizin Berlin (im folgenden Charité genannt) zur Unterstützung von Arbeiten mit experimentellen Modellen für menschliche Erkrankungen in der Biomedizin.
![]("file:///C:/Users/kuehla/AppData/Local/Temp/msohtmlclip1/01/clip_image001.png") |
§1 Allgemeines
- Die iPATH.Berlin ist Teil der zentralen Forschungsdienstleister der Charité. Sie ist am Campus Benjamin Franklin lokalisiert.
- Die iPATH.Berlin stellt sicher, dass alle Erfordernisse guter wissenschaftlicher Praxis und des Datenschutzes berücksichtigt werden. Die Sicherung der Einhaltung ethischer Standards in den Experimenten obliegt den Auftraggebenden.
- Die iPATH.Berlin stellt ihre Leistungen innerhalb und außerhalb der Charité zur Verfügung.
§2 Ziele und Aufgaben
- Im Sinne einer Dienstleistung werden eingesandte Gewebeproben unabhängig von der Kenntnis des experimentellen Aufbaus („geblindet“) bearbeitet und bewertet. Dazu werden innerhalb der iPATH.Berlin histopathologische Techniken stetig weiterentwickelt und standardisiert.
- öffentlich zugänglich gemacht (https://histopathologie.charite.de).
§3 Ordnung
- Die Nutzerordnung regelt:
- die Organisation von Dienstleistungen und die Forschung der iPATH.Berlin, einschließlich damit verbundener Arbeitsabläufe.
- die Beziehungen zu Auftraggebenden.
- Die Nutzerordnung wird durch die wissenschaftliche Leitung der iPATH.Berlin in Abstimmung mit dem Prodekanat für Forschung beschlossen und aktualisiert. Die verbindliche aktuelle Version ist im Webauftritt der iPATH.Berlin veröffentlicht.
- Die Nutzerordnung ist für alle Auftraggebenden verbindlich.
§4 Leitung und Koordination
Die iPATH.Berlin wird durch eine verantwortliche Leitung organisiert und vertreten.
§5 Dienstleistungen
- Im Routinebetrieb bietet die iPATH.Berlin Dienstleistungen in den folgenden Komplexen an:
- Vorbehandeln von Probenmaterial
- Anfertigen von Paraffinblöcken (FFPE-Material) und Nachbearbeiten von Proben bei ungenügender Fixierung, Beschriftung oder Blockfertigung.
- Anfertigen von Paraffin- oder Gefrierschnitten.
- Histochemische Standard- und Sonderfärbungen von Paraffin- oder Gefrierschnitten nach etablierten Protokollen.
- Etablieren von Protokollen für Färbungen mittels (Immun)histochemie, Immunfluoreszenz und in situ-Hybridisierung.
- Beurteilen der Histomorphologie aus Hämatoxylin und Eosin (H&E)-gefärbten Schnitten.
- Manuelle oder computergestützte Auswertung histopathologischer Färbungen.
- Bereitstellen von Fotos und Abbildungen.
- Langzeitlagerung (bis zu maximal 10 Jahren) von FFPE-Material.
- Langzeitlagerung (bis zu maximal 3 Jahren) von Gefriermaterial.
- Kurzzeitlagerung und Versenden von FFPE- oder Gefriermaterial bzw. Rücksenden von Probengefäßen.
- Die Leistungen gemäß §5(1) sind in einem Leistungskatalog weiter spezifiziert. Der aktuelle Leistungskatalog ist im Webauftritt der iPATH.Berlin verfügbar.
§6 Übergabe von Probenmaterial
- Proben können entweder persönlich arbeitstäglich zwischen 9 und 15 Uhr oder per Post bzw. Kurier in die iPATH.Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12203 Berlin, Haus 5011, Raum E812 (= Postanschrift) gebracht werden.
- Eine Sendung mit dem Probenmaterial ist nur vollständig, wenn die Leistungen über OpenIRIS angefordert wurden (https://iris.charite.de).
- Toxisches, humanpathogenes und/oder radioaktives Probenmaterial wird nicht bearbeitet. Auftraggebende haften für die Richtigkeit der Angaben.
§7 Bearbeitungszeit und Übermittlung der Ergebnisse
- Die Bearbeitung der Proben erfolgt nach Datum des Probeneingangs. Diese Abfolge kann nach Absprache mit der Leitung der iPATH.Berlin geändert werden.
- Die Länge der Bearbeitungszeit wird bestimmt durch das aktuelle Gesamtprobenaufkommen, Art und Umfang der beauftragten Leistungen sowie der Verfügbarkeit der benötigten Reagenzien.
- Auftraggebende erhalten die Ergebnisse im vereinbarten Umfang per E-Mail oder via Datenwolke. Die iPATH.Berlin ist für den Verbleib der Daten nicht verantwortlich.
§8 Rücksendung von Probenmaterial
- Eine Rücksendung von Probenmaterial an Auftraggebende muss mittels Leistungsanforderung beauftragt werden. Das Material wird kostenpflichtig an die angegebene Adresse versandt.
- Zehn Werktage nach dem Versenden der E-Mail mit den Ergebnissen aus einem Auftrag beginnt automatisch die kostenpflichtige Langzeitlagerung der Proben aus diesem Auftrag. Es gelten die Preise aus dem aktuellen Leistungskatalog.
§9 Kosten für Dienstleistungen
- 1. Die Kosten für Dienstleistungen der iPATH.Berlin sind in einem Leistungskatalog veröffentlicht.
- verfügbar.
- Die Abrechnung erfolgt quartalsweise mit Rechnungstellung im April, Juli, September und Dezember.
- Die Kosten für Lagerung und Versand werden jährlich erhoben.
- Dienstleistungen der iPATH.Berlin können als Sachbeihilfe bei Drittmittelgebern beantragt werden. Die iPATH.Berlin erstellt für Projektanträge Kostenabschätzungen auf der Basis des Leistungskatalogs in der jeweils aktuellen Fassung.
- Die Preise für das Leistungsangebot werden in regelmäßigen Abständen der Kostenentwicklung angepasst.
§10 Veröffentlichung von Daten
- Die Sicherung und Archivierung der durch die iPATH.Berlin übermittelten Daten obliegt den Auftraggebenden.
- Generell behält sich die iPATH.Berlin auf alle von ihr generierten Daten das Urheberrecht vor.
- Bei Routineanalysen gemäß §5(1), die keine zusätzliche Bewertung beziehungsweise intensive Beratung enthalten, geht das Urheberrecht mit der Bezahlung der Leistung auf den Auftraggebenden über. In wissenschaftlichen Veröffentlichungen in denen solche Daten verwendet werden, ist die Leistung der iPATH.Berlin in den Materials and Methods oder in den Acknowledgments mit folgendem Wortlaut zu vermerken. „Histopathological analysis was provided by the iPATH.Berlin ‑ Immunopathology for Experimental Models, core unit of the Charité – Universitätsmedizin Berlin (Berlin, Germany).”
- Die Veröffentlichung von Daten, die im Rahmen vereinbarter Kooperationen generiert wurden und von Arbeiten infolge eingehender Beratung z.B. bei der Projektplanung oder Datenauswertung wird empfohlen, den intellektuellen Beitrag der iPATH.Berlin durch Autorenschaft(en) zu würdigen.
§11 Gültigkeit
- Die Nutzerordnung gilt ab dem 01. Januar 2021 und ersetzt die Nutzerordnung in der Fassung vom 01.01.2018.
- Änderungen in der Nutzerordnung bedürfen der Zustimmung der Leitung der iPATH.Berlin und dem Prodekanat für Forschung.
PD Dr. Anja A. Kühl
Leitung der iPATH.Berlin
Prof. Dr. Christian Hagemeier
Prodekan für Forschung
Berlin, den 01.01.2021
Provider Contact: anja.kuehl@charite.de; simone.spieckermann@charite.de
Provider Location: Campus Benjamin Franklin (CBF), Berlin
Name: Tecnai G2 20
Resource type: Electron Microscope
Provider: Core Facility for Electron Microscopy
Affiliations: Charité
Description: Suitable for high resolution EM, cryo-EM and tomography.
Contacts: cfem@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy: https://elektronenmikroskopie.charite.de/fileadmin/user_upload/microsites/ohne_AZ/m_cc02/elektronenmikroskopie/Nutzerordnung_01072020.pdf
Provider Contact: cfem@charite.de
Provider Location: Campus Charité Mitte (CCM) and Charité Campus Virchow-Klinikum (CVK), Berlin
Name: Leo TEM 906
Resource type: Electron Microscope
Provider: Core Facility for Electron Microscopy
Affiliations: Charité
Description: Suitable for standard ultrastructural analysis.
Contacts: cfem@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy: https://elektronenmikroskopie.charite.de/fileadmin/user_upload/microsites/ohne_AZ/m_cc02/elektronenmikroskopie/Nutzerordnung_01072020.pdf
Provider Contact: cfem@charite.de
Provider Location: Campus Charité Mitte (CCM) and Charité Campus Virchow-Klinikum (CVK), Berlin
Name: Gemini SEM 300
Resource type: Electron Microscope
Provider: Core Facility for Electron Microscopy
Affiliations: Charité
Description: Suitable for standard ultrastructural analysis.
Contacts: cfem@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy: https://elektronenmikroskopie.charite.de/fileadmin/user_upload/microsites/ohne_AZ/m_cc02/elektronenmikroskopie/Nutzerordnung_01072020.pdf
Provider Contact: cfem@charite.de
Provider Location: Campus Charité Mitte (CCM) and Charité Campus Virchow-Klinikum (CVK), Berlin
Name: 11. AMBIO Nikon Scanning Confocal A1Rsi+
Resource type: Microscope
Provider: AMBIO
Affiliations: Charité
Description:
Imaging modes
- Scanning confocal (DAPI, GFP, mCherry, Cy5)
- FRAP/PA (photo activation/conversion)
- Live-cell incubator (temperature, CO2, humidity)
- Spectral scans
- FLIM/FCS
Lasers and max. intensities out of 60x oil objective
- 405 nm : 0.44 mW
- 488 nm : 0.80 mW
- 561 nm : 1.66 mW
- 647 nm : 1.70 mW
Emission Filters
Filter | λ/Bandwidth |
DAPI | 450/50 |
GFP | 525/50 |
mCherry | 595/50 |
Cy5 | 700/75 |
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 512x512 pixels, up to 420 fps in 512x32 pixels)
- Pinhole: hexagonal, tunable
Detectors
- 2x Hybrid GaAsP PMT (high sensitivity, GFP, mCherry)
- 2x PMT (Dapi, Cy5)
- Transmission light detector (DIC, phase)
- Spectral detector (optical diffraction grid and 32-channel multianodes)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x | Plan Achroat | Air | 0.3 | 10,500 | |
20x | Plan Apo | Air | 0.8 | 1,000 | DIC N2 VC |
40x | Apo | Water | 1.25 | 180 | DIC N2 λ S |
60x | Plan Apo | Water | 1.27 | 170 | DIC N2 IR |
40x | Plan Flour | Oil | 1.3 | 200 | DIC N2 H |
60x | Plan Apo | Oil | 1.4 | 130 | λ |
Special
- Fast switch between galvo and resonant scanners
- Spectral scanner (unmixing)
Contacts: ambio@charite.de
Comments: New users - access upon training request here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: 13. AMBIO Nikon Spinning Disk Confocal CSU-X
Resource type: Microscope
Provider: AMBIO
Affiliations: Charité
Description:
Imaging modes
- Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
- FRAP/ PA (photo activation/ conversion)
- Live-cell incubator (temperature, CO2, humidity)
Lasers and max. intensities out of 60x oil objective
- 405 nm : 3.54 mW
- 488 nm : 3.85 mW
- 561 nm : 4.11 mW
- 647 nm : 4.68 mW
Emission Filters
Filter | λ/Bandwidth |
Quad emission filter (DAPI, GFP, mCherry, Cy5) | 440/40 521/21 607/34 700/45 |
DAPI | 452/45 |
GFP | 525/50 |
mCherry | 600/50 |
Cy5 | 685/40 |
GFP narrow | 510/20 |
Detectors
- 2 EMCCD cameras (iXon3 DU-888 Ultra, 1024x1024 pixels, 13 μm pixel size, 26 fps full frame, usable area ~512x512 pixels, 93 fps)
- Current dual color dichroic for simultaneous GFP & mCherry detection
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x (not installed) | Plan Achroat | Air | 0.3 | 10,500 | |
20x | Plan Apo | Air | 0.8 | 1,000 | DIC N2 VC |
40x | Apo | Water | 1.25 | 180 | DIC N2 λ S |
60x | Plan Apo | Water | 1.27 | 170 | DIC N2 IR |
40x | Plan Flour | Oil | 1.3 | 200 | DIC N2 H |
60x | Plan Apo | Oil | 1.4 | 130 | λ |
100x | Plan Apo | Oil | 1.45 | 130 | λ |
Special
- Andor Revolution SD System (CSU-X)
- Uniform spinning disk illumination
- 2 cameras for ultra-fast dual color imaging
- Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
- Ultra-fast laser switching/ tuning (triggered acquisition)
Contacts: ambio@charite.de
Comments: New users - access upon training request here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: 10. AMBIO Nikon Widefield Ti2
Resource type: Microscope
Provider: AMBIO
Affiliations: Charité
Description:
Imaging modes
- Widefield EPI fluorescence (DAPI, CFP, GFP, YFP, mCherry, Cy5)
- Bright-field for histology slides
- Automated phase for 10x, 20x objectives
- Live-cell incubator (temperature, CO2, humidity)
- Multiplexing (multi position, large image scan, automation)
LED Illumination (Lumencore, SpectraX) and max. intensities out of 40x objective
- 395/25 nm : 66.5 mW
- 438/29 nm : 109 mW
- 475/28 nm : 57.0 mW
- 511/16 nm : 12.3 mW
- 555/28 nm : 145 mW
- 635/22 nm : 38.9 mW
Emission Filters
Filter | λ/Bandwidth |
Quad emission filter (DAPI, GFP, mCherry, Cy5) | 435/33 520/26 595/38 695/63 |
CFP | 473/24 |
YFP | 540/30 |
GFP | 519/26 |
mCherry | 595/31 |
Cy5 | 697/60 |
Detectors
- sCMOS, PCO.edge camera (>80% QE, 2048x2048 pixels, 6.5 µm pixel size, 53 fps full frame, up to 500 fps in small area)
- Nikon DS-Ri2 color CMOS camera (4908x3264 pixels, 6 fps full frame, 45 fps in 1636x1088 pixels)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
4x | Plan Apo | Air | 0.2 | 20,000 | λ |
10x | Plan Flour | Air | 0.3 | 15,200 | Ph1 DL |
20x | Plan Apo | Air | 0.8 | 1,000 | Ph2 DM λ |
40x | Apo Flour | Air | 0.95 | 250 | λ |
Special
- Fast LED laser switching/ tuning (triggered acquisition)
- Fast emission filter wheel for clean multi-color imaging of DAPI, CFP, GFP, YFP, mCherry, and Cy5
Contacts: ambio@charite.de
Comments: New users - access upon training request here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: 17. AMBIO Nikon Multiphoton Confocal
Resource type: Microscope
Provider: AMBIO
Affiliations: Charité
Description:
Imaging modes
- Multiphoton confocal
- DIC, relief contrast
Laser (Mai Tai Insight Deepsee Dual)
- Tunable: 680 nm (600 mW) – 900 nm (500 mW) – 1300 nm (500 mW)
- Single line: 1040 nm
Emission Filters
Filter | λ/Bandwidth |
DAPI | - |
GFP | 525/50 |
mCherry | 595/50 |
Cy5 | 700/75 |
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 512x512 pixels, up to 420 fps in 512x32 pixels)
Non-Descanned Detectors (no pinhole)
- 2x GaAsP-NDD (highly sensitive, GFP, mCherry)
- 2x PMT-NDD (DAPI, Cy5)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
25x | Apo | Water | 1.1 | 2,000 | NCC |
40x | Apo | Water | 0.8 | 3,500 | NIR |
Special
- Upright
- Extended IR laser for deep tissue imaging
- Fast switch between glavo and resonant scanner
Contacts: ambio@charite.de
Comments: New users - access upon training request here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: 16. AMBIO Nikon TIRF/STORM
Resource type: Microscope
Provider: AMBIO
Affiliations: Charité
Description:
Imaging modes
- Widefield EPI fluorescence (DAPI, GFP, mCherry, Cy5)
- Phase (10x, 20x)
- DIC (20x, 40x, 100x)
- TIRF (adhesive cell membrane), motorized TIRF angle
- dSTORM (super-resolution, single molecule localization)
- FRAP/ PA (photo activation/ conversion)
- Live-cell incubator (temperature, CO2, humidity)
Lasers (max. intensities out of 100x objective)
- 405 nm : 3.31 mW
- 488 nm : 53.5 mW
- 561 nm : 37.4 mW
- 647 nm : 80.7 mW
Emission filters
High speed emission filter wheel (OptoSpin IV) for clean multi-color imaging:
Filter | λ/Bandwidth |
GFP | 525/50 |
mCherry | 600/37 |
Cy5 | 635 LP |
Emission splitter (OptoSplit III) for simultaneous 2- or 3-color imaging:
SD-dSTORM cube | |
AF568, mCherry | |
AF647, CF680 | |
Triple cube | |
GFP, AF488 (525/50) | |
AF568, mCherry (600/37) | |
AF647, CF680 (700/75) |
Detectors
- Camera 1 (left port): EMCCD, Andor iXon Ultra X3 987, 512x512 pixels, 16 µm pixel size, 56 fps full frame, QE max. 90%
- Camera 2 (right port): sCMOS, Photometrics Prime 95B (95% QE, 1200x1200 pixels, 11 µm pixel size, 41 fps full frame, 82 fps@12-bit)
- Camera 3 (left port front): CMOS, The Imaging Source DMK 23UX249 (1920x1200 pixels, 5.86 µm pixel size, 30 fps full frame)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x | Plan Flour | Air | 0.3 | 15,200 | Ph1 DL |
40x | Plan Flour | Oil | 1.3 | 240 | DIC H N2 |
60x | Plan Apo | Oil | 1.4 | 130 | λ |
100x | Plan Apo | Oil | 1.5 | 120 | DIC H N2 TIRF |
(All objectives have an external phase contrast)
Special
- 2x beam condenser for small FOV (dSTORM)
- Ultra-fast laser switching/ tuning (triggered acquisition)
Contacts: ambio@charite.de
Comments: New users - access upon training request here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: 15. AMBIO Nikon Upright Spinning Disk Confocal S2
Resource type: Microscope
Provider: AMBIO
Affiliations: Charité
Description:
Imaging modes
- Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
- Upright
Lasers and max. intensities out of 25x objective
- 405 nm : 6.3 mW
- 488 nm : 7.7 mW
- 561 nm : 9.7 mW
- 647 nm : 9.5 mW
Detectors
- Zyla 4.2 Plus sCMOS camera (2048x2048 pixels, 40 fps@16-bit, 53 fps@12-bit)
Emission Filters
Filter | λ/Bandwidth |
Quad emission filter (DAPI, GFP, mCherry, Cy5) | 440/20 521/20 607/20 700/20 |
DAPI | 447/60 |
GFP | 525/25 |
mCherry | 600/52 |
Cy5 | 708/75 |
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x | Plan Achroat | Water | 0.45 | 4,000 | λ |
25x | Apo | water | 1.10 | 2,000 | NCC |
Special
- Andor Revolution SD System (CSU-X1)
- Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
- Ultra-fast laser switching/ tuning (triggered acquisition)
Contacts: ambio@charite.de
Comments: New users - access upon training request here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: Ultimaker S5
Resource type: 3D Printer
Provider: Central Scientific Workshop
Affiliations: Charité
Description: Discover the easy-to-use desktop 3D printer with a large build volume
that delivers accurate, industrial-grade parts, time and again. With
simple setup, high uptime, and reliable dual extrusion, Ultimaker S5 is
the complete professional 3D printing solution.
Fused deposition modeling (FDM) for PLA, ABS, PC filaments and others.
Schmelzschichtung (FDM) für PLA, ABS, PC-Filament und andere.
Contacts: jan-erik.ode@charite.de
Comments: Only operated by facility staff.
Provider Contact: cww@charite.de
Provider Location: CVK, Berlin Wedding and CCM, Berlin Mitte
Name: Device 1 | PharmaScan 70/16 US
Resource type: Magnetic Resonance Imaging System
Provider: 7T Experimental MRIs
Affiliations: Charité
Description: Device 1 | PharmaScan 70/16 US (installation 03/2003; upgrade 04/2013)
Bruker Software Paravision 5.1
Contacts: expmrt@charite.de
Comments: Access to the equipment is only granted upon a service request (see services here).
Bookings have to be approved by the facility (indicated by '-'). You will receive an email once your booking is approved.
Provider UsagePolicy: https://neurocure.de/id-7t-animal-mri-204.html?file=files/neurocure/Z_Core%20Facilities/7T%20Experimental%20MRI/Nutzerordnung_7T_Exp_MRT_R03.pdf
Provider Contact: philipp.boehm-sturm@charite.de
Provider Location: Campus Charité Mitte (CCM), Berlin
Name: Device 2 | BioSpec 70/20 USR
Resource type: Magnetic Resonance Imaging System
Provider: 7T Experimental MRIs
Affiliations: Charité
Description: Device 2| BioSpec 70/20 USR (installation 08/2013)
Bruker Software Paravision 6.0.1
Contacts: expmrt@charite.de
Comments: Access to the equipment is only granted upon a service request (see services here).
Bookings have to be approved by the facility (indicated by '-'). You will receive an email once your booking is approved.
Provider UsagePolicy: https://neurocure.de/id-7t-animal-mri-204.html?file=files/neurocure/Z_Core%20Facilities/7T%20Experimental%20MRI/Nutzerordnung_7T_Exp_MRT_R03.pdf
Provider Contact: philipp.boehm-sturm@charite.de
Provider Location: Campus Charité Mitte (CCM), Berlin
Name: Formlabs Form 2
Resource type: 3D Printer
Provider: Central Scientific Workshop
Affiliations: Charité
Description: The Form 2 delivers high-resolution parts at a fraction of the cost and
footprint of industrial 3D printers. Scale your prototyping and
production in-house with desktop stereolithography.
Stereolitography (SLA) for modern synthetic resins.
Stereolithografie (SLA) für moderne Kunstharze.
Contacts: jan-erik.ode@charite.de
Comments: Only operated by facility staff.
Provider Contact: cww@charite.de
Provider Location: CVK, Berlin Wedding and CCM, Berlin Mitte
Name: Markforged Onyx Pro
Resource type: 3D Printer
Provider: Central Scientific Workshop
Affiliations: Charité
Description: The Onyx Pro prints composite parts that are
10x the strength of plastic by reinforcing chopped-carbon nylon with
continuous strands of fiberglass.
Suitable for nylon with carbon fiber and optionally glass fiber.
Geeignet für Nylon mit Karbon-Fasern und optional Glasfaser.
Contacts: jan-erik.ode@charite.de
Comments: Only operated by facility staff.
Provider Contact: cww@charite.de
Provider Location: CVK, Berlin Wedding and CCM, Berlin Mitte
Name: CO2 Laser Cutter SH-G6090 150W (Sabko GmbH)
Resource type: Laser Cutter
Provider: Central Scientific Workshop
Affiliations: Charité
Description: CO2 Laser Cutter SH-G6090 150W (Sabko GmbH)
Suitable for acrylic glass and POM.
Geeignet für Acryl-Glas und POM.
Contacts: jan-erik.ode@charite.de
Comments: Only operated by facility staff.
Provider Contact: cww@charite.de
Provider Location: CVK, Berlin Wedding and CCM, Berlin Mitte
Name: Project Meetings - Imaging Mass Cytometry (Hyperion)
Resource type: Project Discussion
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
Imaging Mass Cytometry (IMC) is an approach for the targeted and epitope-specific multiparameter analysis of tissue sections (Scheme). In contrast to conventional immunohistochemistry or immunofluorescence, this technology allows for the simultaneous detection of >35 specific markers on the tissue by using metal-isotopes and their different masses as analytes. Tissue sections are labeled with epitope-specific, metal isotope conjugated antibodies and the tissue is ablated in 1x1 µm spots by a laser. The ablated material is carried into the mass cytometer, and the metal ion content of each pixel is analyzed by time-of-flight mass spectrometry. The signals are then plotted as a multiparameter image that can be analyzed by any imaging software. For more information about this new technology contact the core facility.
Measurement of tissue samples
IMC is offered as service (staff-operated) or independent usage with assistance. A project meeting is required to get access. We will discuss your experimental setup, technical details and the timeline for your sample processing. Please request a project meeting as early as possible, but latest 6 weeks before onset of your experiment.
Contacts: desiree.kunkel@bih-charite.de
Comments:
Please download the project registration form and the table for your first panel draft and attach it to the request.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: Hyperion - Imaging Mass Cytometer
Resource type: Imaging Mass Cytometer
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
Imaging Mass Cytometry (IMC) is an approach for the targeted and epitope-specific multiparameter analysis of tissue sections (Scheme). In contrast to conventional immunohistochemistry or immunofluorescence, this technology allows for the simultaneous detection of >35 specific markers on the tissue by using metal-isotopes and their different masses as analytes. Tissue sections are labeled with epitope-specific, metal isotope conjugated antibodies and the tissue is ablated in 1x1 µm spots by a laser. The ablated material is carried into the mass cytometer, and the metal ion content of each pixel is analyzed by time-of-flight mass spectrometry. The signals are then plotted as a multiparameter image that can be analyzed by any imaging software. For more information about this new technology contact the core facility.
Measurement of tissue samples
IMC is offered as service (staff-operated) or independent usage with assistance. Measurement of tissue sections can be requested via OpenIRIS ('Services' - 'Imaging Mass Cytometry - Sample Acquisition') with a date/deadline and will then be coordinated by the core facility. Longer measurements usually run overnight or over the weekend. Please request measurements as early as possible.
funded by DFG: INST 335/658-1 FUGG
Contacts: yasmina.rodriguez-sillke@bih-charite.de
Comments:
Usage of imaging mass cytometry can be requested in "services".
Check CyTOF Scheduler for your bookings and available slots.
Please contact the core facility at least 6 weeks before starting new projects to discuss technical details and instrument availability.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: Project Meetings - Flow Cytometry
Resource type: Project Discussion
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
Contacts: desiree.kunkel@bih-charite.de
Comments:
Project discussion before use of analysers and cell sorting service.
Please download the registration form and attach it to the request.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: Project Meetings - Mass Cytometry
Resource type: Project Discussion
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
Contacts: desiree.kunkel@bih-charite.de
Comments:
Please download the project registration form and CyTOF panel template and attach it to the request.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: Imaging Mass Cytometry - Sample Acquisition
Resource type: Imaging Mass Cytometry
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
Imaging Mass Cytometry (IMC) is an approach for the targeted and epitope-specific multiparameter analysis of tissue sections (Scheme). In contrast to conventional immunohistochemistry or immunofluorescence, this technology allows for the simultaneous detection of >35 specific markers on the tissue by using metal-isotopes and their different masses as analytes. Tissue sections are labeled with epitope-specific, metal isotope conjugated antibodies and the tissue is ablated in 1x1 µm spots by a laser. The ablated material is carried into the mass cytometer, and the metal ion content of each pixel is analyzed by time-of-flight mass spectrometry. The signals are then plotted as a multiparameter image that can be analyzed by any imaging software. For more information about this new technology contact the core facility.
Measurement of tissue samples
IMC is offered as service (staff-operated) or independent usage with assistance. Measurement of tissue sections can be requested via OpenIRIS ('Services' - 'Imaging Mass Cytometry - Sample Acquisition') with a date/deadline and will then be coordinated by the core facility. Longer measurements usually run overnight or over the weekend. Please request measurements as early as possible.
Contacts: yasmina.rodriguez-sillke@bih-charite.de
Comments: Please request a project meeting at least 6 weeks before start of a new project to get access.
The sample acquisition template -> new version!! (download) has to be uploaded when submitting the request and a description of the slide has to be provided (see example here).
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: nanoSPECT/CT plus
Resource type: Preclinical Imaging System
Provider: BERIC
Affiliations: Charité
Description:
- Resolution-booster™: the first ground breaking total detection chain design principle
- Patented* M3-pinhole™ and Tera-Tomo™ 3D SPECT reconstruction technology
- x2 gain in SPECT sensitivity achieve 13.000 cps/MBq
- x2 gain in volumetric SPECT resolution
Contacts: nicola.beindorff@charite.de
Comments: If you would like to use the instrument, please contact us via email (see contacts) or phone (+49 (0)30 450 557013).
Provider Contact: nicola.beindorff@charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK), Berlin
Name: nanoScan PET/CT
Resource type: Preclinical Imaging System
Provider: BERIC
Affiliations: Charité
Description:
Contacts: nicola.beindorff@charite.de
Comments: If you would like to use the instrument, please contact us via email (see contacts) or phone (+49 (0)30 450 557013).
Provider Contact: nicola.beindorff@charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK), Berlin
Name: Titan Krios
Resource type: TEM Microscope
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: According to the procurement
regulations of the DFG, access to the Titan
Krios is primarily reserved to the group of applicants of the 91b initiative until the end of 2024.
You can request access to single particle data acquisition on the Titan Krios microscope via the services 'Participate in SPA data acquisition (FU-Berlin)'.
After confirmation, you can request beam time for SPA via the service ´SPA data acquisition (FU Berlin)´.
Contacts: Dr. Tarek Hilal (tarek.hilal@fzem.fu-berlin.de)
Affiliated Communities: TEM users, ACEM
Comments:
There is no direct access to this microscope!
You can request assisted and unassisted SPA measurements at the Titan Krios microscope.
Click the magnifying glass on the left for more details.
Provider UsagePolicy: https://www.biosupramol.de/Ressourcen/Dokumente/BSM_Nutzerregelung.pdf
Provider Contact: kai.ludwig@fzem.fu-berlin.de
Provider Location: Fabeckstraße 36A, 14195 Berlin, Germany
Name: FEI Vitrobot Mark IV (FZEM)
Resource type: Freeze Plunger
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Booking of the Vitrobot is restricted to the group of TEM users only. If you are not member of this group please contact the resource admin(s).
Unassisted preparations are reserved to skilled (trained) users, otherwise a qualified operator is mandatory.
Training for unassisted operations can be requested from the timeline view in the scheduler (top right, booklet symbol). You will be informed after submitting a training request.
Due to the pandemic training is not offered at the moment.
Contacts: Jörg Bürger (joerg.buerger@fzem.fu-berlin.de)
Affiliated Communities: TEM users, ACEM
Comments:
Please note the restricted access to this instrument!
Click the magnifying glass on the left for more details.
! Bookings become valid only after approval by the staff !
Provider UsagePolicy: https://www.biosupramol.de/Ressourcen/Dokumente/BSM_Nutzerregelung.pdf
Provider Contact: kai.ludwig@fzem.fu-berlin.de
Provider Location: Fabeckstraße 36A, 14195 Berlin, Germany
Name: Talos L120C
Resource type: TEM Microscope
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Booking of the Talos L120C (conventional and cryo EM) is restricted to registered members of the BioSupraMol core facillity. Registration can be requested via the Service:
´Register to TEM (BSM)´.
After registration, booking is possible in 4 hour time slots (9:00 to 13:00 and 13:00 to 17:00) from Monday to Friday. (Rare exceptions are matter of agreement.) Unassisted measurements are reserved to skilled (trained) users, otherwise a qualified operator is mandatory and can be chosen during the booking process. It is best practice to make an appointment with the operators before booking assisted measurements.
Training for unassisted microscope operation can be requested from the timeline view in the scheduler (top right, booklet symbol). You will be informed after submitting the training request.
Contacts: Dr. Boris Schade (boris.schade@fzem.fu-berlin.de)
Affiliated Communities: TEM users, ACEM
Comments:
Please note the restricted access to this microscope!
Bookings are only possible from Monday to Friday at two blocks (9:00 to 13:00 and 13:00 to 17:00). Click the magnifying glass on the left for more details.
! Bookings become valid only after approval by the staff !
Provider UsagePolicy: https://www.biosupramol.de/Ressourcen/Dokumente/BSM_Nutzerregelung.pdf
Provider Contact: kai.ludwig@fzem.fu-berlin.de
Provider Location: Fabeckstraße 36A, 14195 Berlin, Germany
Name: SPA data acquisition - Titan Krios (FZEM - SFAB)
Resource type: Service
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Beam time on the Titan Krios microscope is only accesible by this service request.
Every request demands a brief description of the sample and the preliminar work, and must be approved by the group head of the requester. Only after this confirmation, the service can be initiated. We will then, together with the requester, decide on the
necessary steps prior to the data acquisition.
currently, booking of the microscope is based on the usage policies of the Core Facility BioSupraMol.
Contacts: Dr. Boris Schade (boris.schade@fzem.fu-berlin.de), Dr. Tarek Hilal (tarek.hilal@fzem.fu-berlin.de)
Affiliated Communities: TEM users, ACEM
Comments:
Please note the restricted access to the SPA workflow!
This request for SPA data acquisition on the Titan Krios 300 kV electron microscope is only possible for registered users.
You can request a registration via the 'Register for Single Particle Analysis (SPA) - SFAB' request.
Provider UsagePolicy: https://www.biosupramol.de/Ressourcen/Dokumente/BSM_Nutzerregelung.pdf
Provider Contact: kai.ludwig@fzem.fu-berlin.de
Provider Location: Fabeckstraße 36A, 14195 Berlin, Germany
Name: Talos Arctica
Resource type: TEM Microscope
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description:
Contacts: Dr. Kai Ludwig (kai.ludwig@fzem.fu-berlin.de)
Affiliated Communities: TEM users, ACEM
Comments:
Direct access is restricted to FZEM members!
Measurements and beam times are accessible via the services:
'TEM measurements (FZEM - BSM)´.
Provider UsagePolicy: https://www.biosupramol.de/Ressourcen/Dokumente/BSM_Nutzerregelung.pdf
Provider Contact: kai.ludwig@fzem.fu-berlin.de
Provider Location: Fabeckstraße 36A, 14195 Berlin, Germany
Name: Incucyte DKTK CBF
Resource type: Cell Analyzer
Provider: AG Siegmund
Affiliations: Charité
Description: Satorius Live Cell analyzer
Contacts: rainer.glauben@charite.de
Comments:
Everyone can use it, but DKTK members have precedence. If you're a new user, please contact "rainer.glauben@charite.de" for an introduction.
Provider Contact: rainer.glauben@charite.de
Provider Location: Campus Benjamin Franklin
Name: Participate in SPA data acquisition - SFAB
Resource type: Administration (BSM/SFAB - FU)
Provider: Administration OpenIRIS@FUB (BCP)
Affiliations: FU Berlin
Description: According to the procurement regulations of the DFG, access to the Titan Krios is primarily reserved to the group of applicants of the 91b initiative until the end of 2024.
Contacts: Dr. Boris Schade (boris.schade@fzem.fu-berlin.de), Dr. Tarek Hilal (tarek.hilal@fzem.fu-berlin.de)
Comments:
This request addresses to group heads and PI's only
Requesting admission to the SPA workflow enables your group members to register for the SPA data acquisition service at FZEM. Until the end of 2024, the SPA workflow is primarily reserved to 91b Titan Krios applicants.
Members of registered groups must, however, only use the 'Register to Single Particle Analysis (SPA) - SFAB' request to get access.
Provider Contact: boris.schade@fzem.fu-berlin.de
Provider Location: Fabeckstraße 36A, 14195 Berlin
Name: Project request
Resource type: Project
Provider: High-Throughput Mass Spectrometry
Affiliations: Charité
Description: Please request an initial meeting at least 4 weeks before the start of a new project.
Contacts: michael.muelleder@charite.de
Provider Contact: michael.muelleder@charite.de
Provider Location: Campus Charité Mitte (CCM), Berlin
Name: ThermoFisher Vitrobot Mark IV
Resource type: Plunge Freezer
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Key Benefits:
- Fully automated, reproducible vitrification of aqueous suspensions
- Precise control of critical process parameters
- Enclosed process chamber
- High sample throughput
- Easy and flexible user interface
- Semi-automated grid transfer
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: ThermoFisher Titan Krios G3i
Resource type: cryo-Transmission Electron Microscope
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Key Features:
- 80 - 300 keV Acceleration Voltage
- X-FEG Electron Source
- Twelve cartridge Auto Loading System
- Ceta 16Mpx CMOS Camera
- Falcon 3 DED
- GATAN K3 DED + BioQuantum GIF
- Volta Phase Plate
- Dual Axis Tomography Holder
- Software Packages: EPU, Tomo5, SerialEM, Maps
- Live pre-processing of raw data
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: ThermoFisher Aquilos
Resource type: cryo-FIB/SEM
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Key Features:
- Complete cryo infrastructure including cryo transfer station
- Fully rotatable cryo-stage
- Special Cryo-FIB Autogrids for shallow-angle milling
- Sample shuttle for Autogrids: Cryo-FIB shuttle with integrated shutter system during cryo-transfers
- In-chamber retractable sputter coater for applying conductive coatings
- GIS-system for applying protective coatings
- Including MAPS software for correlative workflow
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: LEICA TCS SP8
Resource type: cryoCLEM
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Key Features:
- Complete cryo Infrastructure including cryo transfer station
- HCX PL APO 50x CLEM cryo-Objektive
- SP8 confocal optical system + SP8 LIGHTNING software
- Compatible with MAPS software for correlative workflow
Laser Lines
405,488,552,638
Detectors
1x PMT detector, 2x HyD detectors
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: 3T MRI system MRS 3017
Resource type: Magnetic Resonance Imaging System
Provider: BERIC
Affiliations: Charité
Description: The 3T Benchtop MRI (MRsolutions MRS 3017; date of manufacture 2015) has a high-performance gradient system and a comprehensive selection of coils. The system is suitable for both cardiac and non-cardiac imaging studies in mice and rats and is compatible with multinuclear imaging (fluorine, xenon, etc.).
Contacts: nicola.beindorff@charite.de; daniel.messroghli@charite.de
Comments: If you would like to use the instrument, please contact us via email (see contacts) or phone (+49 (0)30 450 557013).
Provider Contact: nicola.beindorff@charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK), Berlin
Name: Luminescent Image Analyzer
Resource type: Equipment
Provider: AG Siegmund
Affiliations: Charité
Description:
Contacts: rainer.glauben@charite.de
Comments: everyone can use it
Provider Contact: rainer.glauben@charite.de
Provider Location: Campus Benjamin Franklin
Name: Real-Time PCR Machine (StepOne)
Resource type: qPCR
Provider: AG Siegmund
Affiliations: Charité
Description:
Contacts: inka.freise@charite.de
Comments: everyone can use it
Provider Contact: rainer.glauben@charite.de
Provider Location: Campus Benjamin Franklin
Name: Aurora "Rory" - Full Spectrum Analyzer
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
Contacts: jacqueline.keye@bih-charite.de
Comments:
You need to be registered and trained to use this instrument.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: SU 8030
Resource type: SEM
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Booking of the SU8030 (cryo and conventional SEM) is restricted to registered users. Registration can be requested via the service:
´Register to SEM (BSM)´.
After registraition, booking times are are available on the hour from 8:00 to 18:00 (Fri 17:00) in blocks of 60 minutes only. Durations can range from 1 hr up to a maximum of 10 (9) hrs. (Rare exceptions are matter of agreement.) Unassisted measurements are reserved to skilled (trained) users, otherwise a qualified operator is mandatory, which may be chosen during the booking process. It is best practice to make an appointment with the operators before booking assisted measurements.
Training for unassisted microscope operation can be requested from the timeline view in the scheduler (top right, booklet symbol). You will be informed after submitting a training request.
Contacts: Anke Schindler (anke.schindler@fu-berlin.de)
Affiliated Communities: TEM users, ACEM
Comments:
Please note the restricted access to this microscope!
Booking times are on the hour from 8:00 to 18:00 (Fri 17:00) in blocks of 60 minutes only. Click the magnifying glass on the left for more details.
! Bookings become valid only after approval by the staff !
Provider UsagePolicy: https://www.biosupramol.de/Ressourcen/Dokumente/BSM_Nutzerregelung.pdf
Provider Contact: kai.ludwig@fzem.fu-berlin.de
Provider Location: Fabeckstraße 36A, 14195 Berlin, Germany
Name: Leica DMi8
Resource type: Light microscopy
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description:
Inverted microscope for Live-cell imaging
- Incubation chamber for Temperature and CO2 control
- Adaptive Focus Control (AFC)
- DFC9000 GT Camera
- Available to use with the PRIMO device
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: PRIMO Alveole for micropatterning
Resource type: Other Instruments
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description:
A device for well-positioned cells within the mesh of the EM grids, and several other applications.
- protein micropatterning
- hydrogel structuration
- microfabrication
Please feel free to contact us for further information on the technique and applications.
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: FIB-milling with Aquilos2 Training
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Obligatory training for all users who wish to use the Aquilos microscope
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: PRIMO training
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Obligatory training for all users who want to use the PRIMO micropatterning device
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: Training for Vitrobot
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Obligatory training for all users who want to use the Vitrobot machine.
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: Training for cryoCLEM microscopy
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Obligatory training for all users who want to use the cryo confocal SP8
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: Training on cryo TEM (Krios)
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Obligatory training for all users who want to use the Titan Krios
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: Graphics Workstation
Resource type: Workstation
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charité
Description: Windows GPU workstation
Contacts: christoph.diebolder@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy:
Nutzerordnung für die
Core Facility für cryo-Elektronenmikroskopie (CFcryoEM)
Charité – Universitätsmedizin Berlin Campus Buch
- Präambel
Die Core Facility für Kryo-Elektronenmikroskopie (CFcryoEM) der Charité - Universitätsmedizin Berlin bietet das komplette Spektrum an Transmissions- und Rasterelektronenmikroskopischen Methoden (TEM, REM/SEM) unter kryogenen Temperaturen für biologische Fragestellungen an. Dabei kann der gesamte kryo-elektronentomographische Arbeitsablauf durchgeführt werden, der korrelative Licht- und Elektronenmikroskopie (cryo-CLEM), Focused-Ion-Beam-Rasterelektronenmikroskopie (cryo-FIB-SEM) und Elektronentomographie (cryo-ET) beinhaltet. Wir beraten die Nutzer sowohl bei der Projektplanung und -durchführung (Probenvorbereitung, und -präparation) als auch bei der Auswertung der erhobenen Daten. Dazu weisen wir die Nutzer an den Geräten ein, sodass ein eigenständiges Arbeiten möglich ist (Anwendungsbetrieb). Auf Wunsch und nach Kapazität bieten wir die Probenpräparation und Datenaquise als Serviceleistung an (Servicebetrieb), wobei sämtliche Schritte von Mitarbeitern der CFcryoEM durchgeführt werden. Desweiteren bieten wir Kryo-Elektronenmikroskopie von Einzelmolekülen (single-particle cryo-EM) an.
Perspektivisch ist geplant die CFcryoEM in das Berlin Integrative Structural Biology Network (BIS) zu überführen und somit, zusammen mit Partnern von der Charité, FU Berlin und HU Berlin, die Berlin-weite Infrastruktur für Hochleistungs-Elektronenmikroskopie zu vereinen.
Die vorliegende Nutzerordnung der CFcryoEM ist für alle Nutzer verbindlich. Alle Nutzer verpflichten sich eine Nutzervereinbarung (hier abrufbar) zu unterzeichnen und die Nutzerordnung zu akzeptieren.
Aktuelle Informationen können auf der Homepage unter: www.cryo-em.charite.de nachgelesen werden.
- Ansprechpartner
Allgemein gültige E-Mailadresse der Core Facility für cryo-Elektronenmikroskopie: cryo-em@charite.de
| Leitung: Herr Dr. Christoph Diebolder | christoph.diebolder@charite.de cryo-em@charite.de | |
| Thiemo Sprink | thiemo.sprink@mdc-berlin.de | |
| NN (Techniker) |
Provider Contact: christoph.diebolder@charite.de; cryo-em@charite.de
Provider Location: Campus Berlin Buch (CBB)
Name: User Registration
Resource type: Registration
Provider: BIH Core Unit pluripotent Stem Cells & Organoids
Affiliations: Charité, BIH
Description: In order to use the services and infrastructure of the BIH Core Facility Stem Cells, users must first register and accept the user regulations. The registration requires the confirmation of the respective group leader. The group leader receives a notification in OpenIRIS and can confirm it here.
Contacts:
Provider UsagePolicy:
Terms of Service
BIH Core Unit pluripotent Stem Cells and Organoids
Valid from 24.05.2024
These Terms govern your access to and use of BIH Core Unit pluripotent Stem Cells and Organoids (CUSCO) services and equipment. Our Services are offered subject to your acceptance, without modification, of all the terms and conditions contained herein and all other operating rules, and procedures that may be published from time to time by us. You agree that we may automatically upgrade our Services, and the Agreement will apply to any upgrades. Please read the Agreement carefully before accessing or using our Services. By accessing or using any part of our Services, you agree to become bound by the Agreement. If you do not agree to all the terms of the Agreement, then you may not access or use our Services.
Your Account
The use of our Services requires an account in the openIRIS system (https://iris.charite.de), hence, you agree to provide us with complete and accurate information when you register for an account and keep the information current. This is important because sometimes we may need to send you notable updates. You will be solely responsible and liable for any activity that occurs under your account. You are responsible for keeping your account information up-to-date and for keeping your password secure. You are responsible for maintaining the security of your account and you are fully responsible for all activities that occur under your account and any other actions taken in connection with our Services. You shall not share or misuse your access credentials. You must immediately notify us of any unauthorized uses of your account or of any other breach of security. We will not be liable for any acts or omissions by you, including any damages of any kind incurred as a result ofsuch acts or omissions. Your account will be active for one year and can then be prolonged.
In addition, the following must be observed before the account is created:
- Before use, a free consultation must take place to assess the need and scope.
- Project-related support must be submitted using a project registration in openIRIS and include project title, description of the expected services, and the client.
- Access to the Infrastructure and Services of CUSCO is approved only to the Users who have registered their projects and themselves as USERS via openIRIS system and receive the specific introduction by the CUSCO personnel.
Following account activation, you as a CUSCO user, agree to adhere to the following obligations:
- with the regulations of the user rules and the specific laboratory and equipment rules (see Service and Equipment).
- to attend the annual safety instructions for the necessary laboratory safety levels.
- to strictly follow the instructions of the CUSCO staff in the CUSCO rooms and when using the equipment.
- to make the CUSCO staff aware of the existence of safety risks related to the experimental material (e.g., pathogenic, infectious, toxic, or radioactive properties or according to Biostoffverordnung (BioStoffV)).
- if necessary, to provide evidence of corresponding notifications and approvals (here in particular the approval of the genetic engineering experiments to be carried out or experiments on animals or humans, if necessary, with the approval of the ethics committee) of research projects and experiments. Patient data are to be handled by the user in compliance with the relevant data protection regulations and are to be handed over to CUSCO exclusively in anonymized form.
- any work with genetically modified organisms (according to Gentechnikgesetz (GenTG)) is only allowed after a prior approval with the CUSCO director.
- if necessary, authorize CUSCO to perform the experiments.
- Operate the equipment and perform the experiments only after the instruction of CUSCO staff.
Service and equipment
Within the framework of its available capacities, the CUSCO performs the following services in particular:
- Project consultation and support
- Isolation of primary cells from patient material
- Reprogramming of cells into human induced pluripotent stem cells (hiPSCs)
- Banking, characterization, and quality control of hPSCs
- Genome editing using CRISPR/Cas
- Establishment of differentiation protocols
- Provision of cells differentiated from hiPSCs
- Establishment of methods for organoid generation and analysis
- Provision of hiPSC reference cell lines
- Provision of reagents
- Provision of infrastructure and equipment
- Training
In addition, CUSCO manages access to and supports, the shared equipment (specific equipment available is listed in openIRIS).
Equipment managed by CUSCO is available to all CUSCO registered users. By accepting these Terms of Service, the users agree to adhere to the following rules of the equipment use:
- Equipment access and training must be requested through openIRIS. Approval of access will be granted only to the users that completed the training.
- All equipment available at CUSCO must be booked through openIRIS before use. Use of equipment without booking is strictly forbidden.
- Equipment booking expires 30 min after the booking time has begun if the user is not present, but usage fees will apply.
- In case the use time exceeds the booking time, additional time must be booked in openIRIS.
- Bookings can be made up to a maximum of 14 days before planned use. Cancellation of a booking can be made up to 24 hours before the start of the booked period. Cancellations made after this time may result in usage fees if no other user books this period.
- Note that repeated absence during booked time, use of device without booking and/or misuse of equipment will result in user access cancellation.
- Users are responsible for immediate removal of data from the CUSCO computers. CUSCO is not responsible for data storage and safety. All data older than four weeks will be automatically deleted from the PCs without further notice.
- It is not allowed to store any kind of data anywhere else on the CUSCO computers other than in the respective directories. All data in other locations (for example on the desktop) will be deleted without further notice.
- Equipment manuals and accessories must remain with the respective device at any time (PDFs of manuals can be provided on request by CUSCO).
- In case of a device malfunction, the CUSCO personnel must be immediately informed. Error messages must be copied or documented by a screenshot and uploaded as an “issue” through openIRIS.
- Cells provided to the user must not be shared with other users or researchers outside of the core facility or without consent of the head of CUSCO.
Liability
All users are liable according to the legal regulations. This applies in particular to damage caused by failure to comply with the obligations incumbent on the users, by non-clarification of security risks or by non-compliance with binding instructions issued by the staff. The responsibility for the quality of the incoming material or incoming data of a project lies with the user.
The liability of CUSCO towards users is limited to intent and gross negligence. The CUSCO do not assume any warranty for the test material.
Genetically modifies organisms (GMO)
Regulations regarding working with and handling genetically modified organisms or materials containing genetically modified organisms are described in the Federal Republic of Germany by the Genetic Engineering Act (Gentechnikgesetz-GenTG).
All users requesting genetically modified material or genetic modification of iPSCs (Service: genetic modification) and/or bringing and working with genetically modified organisms in the CUSCO facility are required to inform the CUSCO personnel about such work and provide a copy of the official permission and registration of the genetic engineering facility / laboratory.
For Charité internal regulations see: https://intranet.charite.de/biosicherheit/startseite/
Publications
Users are obliged to take due account of the work of CUSCO in publications following the generally accepted rules of scientific practice:
- In principle, any external work, e.g., the work of CUSCO, must be identified at the appropriate points in scientific work, e.g., in the material and method part. The payment of fees or charges resulting from service does not replace the corresponding labelling of technical or scientific work.
- Depending on the scope and complexity of the work or in the case of corresponding collaboration agreements, the CUSCO or individual employees must be considered by mentioning them in the acknowledgment[1] or within the framework of a co-authorship. Co-authorship of CUSCO scientists usually requires a scientific contribution that goes far beyond the mere performance of experiments or analyses.
- The CUSCO should be notified before submission of the publication. If necessary, the corresponding files must be made available for inspection beforehand. Also, a copy of the publication in electronic form must be sent to the head of CF Stem Cells after publication.
Exclusion and restriction of use
In particular, the approval for use may be refused, revoked, or further restricted if
- no proper application has been submitted
- the information in the application is not or is no longer correct
- a fixed usage fee is not paid
- the regulations for use, the specific laboratory and equipment rules or instructions of CUSCO staff are violated
- CUSCO is not mentioned or adequately considered in publications according to chapter „Publications“.
The user is not entitled to claims for damages due to the refusal, revocation, or subsequent limitation of admission.
Service Catalogue / Usage Fees
- The fees to be paid by the users for CUSCO services can be found in the current list of fees. Special rates apply to external users.
- For projects regarding technology or method development, including feasibility studies, within the framework of a cooperation, the prices can be reduced or, in exceptional waived at CUSCO staff discretion. Co-authorships of CUSCO staff are expected to be considered in the resulting publications according to their scientific or technical contributions.
- The user fees are charged after receipt of the services. In the case of internal customers, this is done via internal service charging (interne Leistungsverrechnung, ILV). External users always receive an invoice.
- Project leaders can request user fees for funding applications (in DFG applications under the item „Requested other costs“, CUSCO is registered in the DFG Database). The team of CUSCO will be available to assist you with the appropriate cost estimation during the application phase.
- CUSCO reserves the right to issue a partial charge on the failed services. Due to underlying biological limitations, CUSCO is unable to always guarantee the success of the following services:
- Reprogramming
- Genome editing
- Differentiation
CUSCO staff guarantees to perform necessary experimental steps to fulfil the service request. However, in an unlikely event of the service failure, the number of further attempts will be limited as defined by individual service descriptions and the services will be continued only after an explicit confirmation from the User. The user agrees to the payment of charges according to the steps completed by CUSCO in the attempts to fulfil the service.
è The current list of fees is attached as Appendix 3.
Severability clause and validity of the user regulations
The user regulations are binding for all users of CUSCO. Agreements beyond the scope of these Terms of Service are made with external users and must be issued in writing. The Terms of Service are valid in the respective version without limitation until they are replaced by an updated version.
Contact
Director: Dr. Harald Stachelscheid
Charité – Universitätsmedizin Berlin
BIH Core Unit pluripotent Stem Cells & Organoids
Cranach Haus
Augustenburger Platz 1
13353 Berlin
(Visitors: Föhrer Straße 15)
Email: CUSCO@bih-charite.de
Appendix 1: Specific rules for equipment use
MACSQuant VYB:
- Users must use personal accounts. Account sharing is not allowed.
- Personal account will be assigned immediately after user introduction.
- Calibration of the machine is performed by the CUSCO personnel once a month.
MACSQuant Tyto FACS Sorter:
- Users must use personal accounts. Account sharing is not allowed.
- Workspace set-up and advanced training cannot be provided by CUSCO and must be requested by the user to Miltenyi directly. CUSCO only provides access to the Sorter, an account, and general recommendations for equipment use.
- Accessories, like Tyto magnet for cartridge load, must be used at CUSCO lab. It is strictly forbidden to remove equipment from CUSCO lab space.
- No gloves are permitted when working with the system.
- The computers operating this equipment must not be switched off. It is critical for the instrument operation that the computers stay active.
- Use of the computer for applications other than device control is not allowed.
- The use of USB storage devices is prohibited.
- must be trained in the operation of the unit by an experienced user. After this training, CUSCO staff will evaluate the ability of the user to operate the unit properly and permit access.
- Measurement data must be transferred to the Columbus server (http://columbus.charite.de) or OMERO Server (https://omero.charite.de) immediately after the measurement and removed from the measurement computer.
Leica inverted Microscope (User Lab):
- Data must be saved in a folder named after the group/username in the following location:
“Dieser PC > Dokumente > Folder [Group Name] > [ username]”
- Data must be saved in a folder named after the group/username in the following location:
“D:> cap_0.5.1.4182005e > folder [username]”QuantStudio 6:
- Data must be saved in a folder named after the group/username in the following location: “D:UsersPublicDocumentsQuantStudio Real-Time PCR softwareexperiments[Group Name][user name]”
Opera Phenix, Operetta and Columbus Server
- All image data should be moved immediately after acquisition to the central OMERO Server (https://omero.charite.de) of the Charité
Picking hood Microscope (User Lab):
- Data must be directly saved in external source (USB stick) and/or deleted immediately from the computer hard disk after transfer.
Chromium Controller
- Only the use of the Controller and accessories ad-hoc are allowed (chip holder and vortex with adaptor for beads rack).
- Accessories must be used at CUSCO lab; it is not allowed to move them somewhere else.
Molecular biology work bench rules
- Molecular biology bench must be booked via openIRIS when Chromium Controller is used.
- This bench is reserved for single cell experiments. Cryostat
- It is mandatory to clean up the device after use with ethanol 80% as it was instructed during the training. Remnants of embedding medium can damage the device.
Leica LSM SP8
- Use of the computer for applications other than device control is not permitted.
- The use of USB storage devices is prohibited.
- All image data should be moved immediately after acquisition to the central OMERO Server (https://omero.charite.de) of the Charité
INCUCYTE – User rules
- It is mandatory to receive training from CUSCO. Training can be booked using OpenIris.
- Only the use of a personal account is allowed.
- Incucyte software can be downloaded in every Charité PC (instructions will be provided during the training). If needed the Working station (ground floor, room 0.0134) can be used to schedule experiments. It is not permitted to use other CUSCO computers.
- Incucyte use is allowed only during booked time slots. Booking has to be scheduled using OpenIris.
- Booking is mandatory for the whole time of experiment/the plates are in the Incucyte.
- ONly 2 concurrent bookings are possible. Each booking allows the use of 2 (out of 6) Incucyte plate-positions (i.e. 1 user = 1 booking slot = 2 positions of the tray).
- 2 (out of 6) spots of the tray are reserved for single scannings (“Scan now” acquisition type). Therefore, those 2 spots HAVE to be free and only used during the single scan (limited time - single time).
- Each booking (up to 2 plates) can use a schedule of a maximum of 30 minutes duration per hour.
- It is mandatory to leave every 3 hours 1 slot of 30 minutes free for spontaneous measurements
- Do book your slots time ahead (preferably 1 week) to avoid undesirable overlapping between users. Booking can be cancelled until 1 hour before it starts (please contact CUSCO member if needed). Booking cancel within 1 hour prior starting will be charged.
- Plate loading has to be done carefully and holding the tray from below.
- You HAVE to remove your plate after the experiment has finished and delete the acquisition schedule from the Software to allow next measurement. If plate is abandoned (no openIRIS booking visible), CUSCO will discard it without further notice.
- If you notice before starting your experiment that water in the incubator is missing or any other anomaly (not signaled by alarm) please contact a CUSCO member.
- Data will be deleted every 2 months. During this time you can analyze and archive your data after downloading.
- Data export to the ImageArtist server is possible. Please contact CUSCO member to learn how to proceed.
- Do not set for acquisition empty wells, focus will not be found and the whole scanning may stop.
Appendix 2: Specific rules for use Cell culture labs
- It is mandatory to book cell culture safety cabinet in openIRIS before use. In case the use time exceeds the booking time, additional time must be booked in openIRIS.
- Use of PPE (lab coat and gloves) is mandatory in the cell culture labs.
- User responsibilities:
è Refill general consumables, for example: 80% ethanol, paper tissues, glass pipettes, plastic material, etc.
è Dispose of trash as indicated during lab introduction (white bins, yellow glass containers, already full trash bags).
è Empty liquid waste (vacuum container).
è Inform CUSCO personnel if reagents or consumables are running out (use the white board outside of the cell culture room to leave notices). Provide enough time before the material is completely consumed.
è Inform CUSCO personnel if any issue with equipment or space is encountered.
è Keep safety cabinets, incubators, water bath and general space clean.
è Re-fill water in incubator if needed using deionized autoclaved water (red cap bottles).
è Only pipette tips with filter are allowed in the cell culture safety cabinets. Please store only one box of pipette tips per size for each bench and make sure they are closed properly when removed from the safety cabinet.
- It is not allowed to stock a large number of aliquots of reagents from the user projects in the fridge/freezer of the user lab. Keep only what is needed in the space assign to you for a month. Space is limited.
- All reagents, plates, boxes, etc. must be properly and clearly labelled. The label must include: the Group, Initials and Date. CUSCO personnel will discard all unidentifiable material without further notice.
- It is not allowed to store samples (DNA, RNA, cell pellets, imaging plates, etc.) in fridge/freezer of the user lab for long term. When a user is not working in the facility anymore (not active) and after notification, we will remove and discard all reagents/samples of the user. Do not store any belongings on the benches/cupboards, they will be removed. It is a common working area.
- After completion of work:
- Clean bench and disinfect the surface with 80% ethanol!
- Clean the tube of the pump first with deionized water and subsequently with 80% ethanol after usage
- Switch off lamps of the microscopes according to the introduction!
- If necessary (last user in the cell culture lab), switch off the bench, pump, water bath and microscope.
- The following materials will be provided by CUSCO and are included in the bench usage fee:
- Serological Pipettes (5ml, 10ml and 25ml)
- Falcon Tubes (15ml and 50ml)
- Plates (6-wells and 12-wells)
- Filter Tips
- Ethanol
- Yellow waste bins
- Gloves
- EDTA solution
- Eppendorf tubes
Furthermore, the following reagents are provided but must be purchased in openIRIS prior use:
- Vitronectin
- ROCK inhibitor
- mTeSR medium
- E8 medium
Appendix 3:Price list of CUSCO internal users (billing via ILV)
| Description | Unit | Price [€] |
|---|---|---|
| Generation of iPSCs, Banking and Characterisation | ||
| Primary cell Isolation | Donor | 90,00 |
| Derivation of iPSCs from primary cells | Donor | 3550,00 |
| Banking of iPS cell line (48 vials) | cell line | 640,00 |
| QC and Characterisation - basic Panel | cell line | 1230,00 |
| Cell Line Identity (STR Analysis) | cell line | 150,00 |
| Virtual Karyotyping using a SNP Array | cell line | 280,00 |
| G-Banding Karyotyping | cell line | 450,00 |
| Morphology and Viability | cell line | 290,00 |
| Sterility and Mycoplasma testing | cell line | 70,00 |
| Marker for undifferentiated iPSCs (IF) | cell line | 340,00 |
| Marker for undifferentiated iPSCs (FACS) | cell line | 400,00 |
| Differentiation capacity of hPSCs | cell line | 640,00 |
| Confirmation of gene editing | cell line | 80,00 |
| Virologic testing of cultured cells | cell line | 510,00 |
| Bank of single cell culture adapted hPSC | cell line | 1710,00 |
| Differentiated Cells and Organoids | ||
| Generation of endothelial cells | BATCH | 1250,00 |
| Generation of hepatocytes | PLATE | 720,00 |
| Generation of neural stem cells | BATCH | 1390,00 |
| Generation of iNeurons (NGN2 induced) | BATCH | 480,00 |
| Celebral organoids - StemCellTech Kit | BATCH | 1460,00 |
| Generation of human Lung Organoids (hLOA) | BATCH | 980,00 |
| Passaging and expansion of hLOA | BATCH | 200,00 |
| Generation of human Intestinal Organoids (hIO) | BATCH | 580,00 |
| Passaging and expansion of hIO | BATCH | 420,00 |
| Gene editing and complex projects | ||
| Supervision | hour | 41,00 |
| CRISPR Design | cell line | 210,00 |
| gRNA Efficiency testing | cell line | 650,00 |
| CRISPR experiment | cell line | 530,00 |
| Single cell cloning with IOTA | cell line | 1000,00 |
| Clone Validation | cell line | 1380,00 |
| Provision of reagents | ||
| E8 Media (500ml) - own production | BOTTLE | 84,00 |
| E8 Media (500ml) - own production KIT | BOTTLE | 84,00 |
| mTeSR Media (500ml) - own production | BOTTLE | 121,00 |
| mTeSR Media (500ml) | BOTTLE | 395,00 |
| Geltrex Aliquots (10 ml) | Aliquot | 82,00 |
| Vitronectin Aliquots (600 µl) | Aliquot | 81,00 |
| ROCK Inhibitor Y-27632 (1 ml) | Aliquot | 68,00 |
| Provision of cells | ||
| Provision of live iPSCs | WELL | 21,00 |
| Cryopreserved iPCSs | VIAL | 79,00 |
| Cryopreserved iPCSs - own bank | VIAL | 15,00 |
| Cryopreserved Neuronal Stem Cells | VIAL | 30,00 |
| Cryopreserved Endothelial Cells | VIAL | 44,00 |
| Devices | ||
| FACS Analyzer MACS Quant VYB | hour | 13,00 |
| FACS Sorter MACS Quant Tyto | hour | 26,00 |
| Real-time PCR Quantstudio6 | hour | 8,00 |
| 10X Chromium Controller | hour | 63,00 |
| Leica Confocal SP8 | hour | 13,00 |
| Opera Phenix High Content Screener | hour | 16,00 |
| Opera Phenix High Content Screener-off h | hour | 5,00 |
| IsoCell | hour | 27,00 |
| TapeStation | hour | 8,00 |
| Incucyte SX5 | hour | 3,00 |
| Cell3Imager Duos | hour | 13,00 |
| Bench fee - User-Lab | hour | 10,00 |
| Training | ||
| Equipment training | hour | 47,00 |
| Hands-on training - Basic hPSC culture | training | 620,00 |
| Hands-on training - iNeurons | training | 530,00 |
| Hands-on training - NSCOs | training | 320,00 |
| Hands-on training - hLOA generation | training | 380,00 |
| Hands-on training - hIO generation | training | 500,00 |
| Hands-on training - Endothelial cells | training | 780,00 |
[1] Please refer to the following formulation example: „This work was supported by the Core Unit pluripotent Stem Cells and Organoids of the Berlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin.”
Provider Location: Charité Campus Virchow-Klinikum (CVK), Berlin
Name: Symphony A3 "Polyhymnia"
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
funded by DFG INST 335/757-1 FUGG
5-Laser Flow Cytometry Analyser
Please request a project meeting and upload the registration form for your sample material to get access.
A training is required for independent usage.
Instrument Configuration (download)
Contacts: tamisa.honda@bih-charite.de
Comments:
You need to be registered and trained to use this instrument.
For analysis of GMO material contact the BIH CCF staff to discuss formalities.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Instrument configuration has changed! (see here)!
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: Cell Sorter - AriaFusion "Terpsichore"
Resource type: Flow Cytometry Sorter
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
funded by DFG / INST 335/758-1 FUGG
4-Laser Sorter, equipped with 15 fluorescence parameters.
Cells can be sorted in bulk (up to 4 populations simultanously) or as single cells in 96well plates / 384well plates. Index Sorting is available.
Please request a project meeting and upload a registration form for your sample material to get access to cell sorting.
Instrument Configuration (download)
Contacts: cytometry-core@bih-charite.de
Comments:
Please request a project meeting to get access to cell sorting services.
Please select your slot and wait for approval. Your booking is not guaranteed until you get notification of approval.
Cancel your booking 48 hours ahead to avoid charges.
For ad-hoc, short notice bookings (next 48 hours) please contact us directly.
Please note: UV-Laser is no longer available on this instrument. See updated configuration here.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: Eastwood Computer
Resource type: Computer
Provider: AG Siegmund
Affiliations: Charité
Description: everyone can use it
Contacts: adrian.huck@charite.de
Comments: everyone can use it
Provider Contact: rainer.glauben@charite.de
Provider Location: Campus Benjamin Franklin
Name: Seahorse
Resource type: Cell Analyzer
Provider: AG Siegmund
Affiliations: Charité
Description: everyone can use it
Contacts: hsiang-jung.hsiao@charite.de
Comments: everyone can use it
Provider Contact: rainer.glauben@charite.de
Provider Location: Campus Benjamin Franklin
Name: OMERO User Access Request (Charité/BIH only)
Resource type: Training
Provider: OMERO Microscopy Image Data Management
Affiliations: Charité, BIH
Description: Request for new users to gain access to the OMERO image repository system (for users internal to Charité/BIH).
Contacts: Omero support
Comments: Please submit access request to get an account on OMERO+ (for users internal to Charité/BIH).
Provider Location: Charité Universitätsmedizin Berlin
Name: OMERO Training course sign-up
Resource type: Request
Provider: OMERO Microscopy Image Data Management
Affiliations: Charité, BIH
Description: OMERO training sessions from basics on how to get started with OMERO to more advanced usage topics.
Contacts: OMERO support
Provider Location: Charité Universitätsmedizin Berlin
Name: TEM measurements (FZEM - BSM)
Resource type: Service
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Service to performing TEM measurements (including phase plate, automated data acquisition, and electron tomography) at the Forschungszentrum für Elektronenmikroskopie (FZEM). Decisions on the requests are made by the staff at the FZEM. The requester will be informed of the
decision.
Skilled (trained) users can perform non-assisted measurements, otherwise a qualified operator is mandatory, which can be recommended in the request.
Booking of this service is based on the usage policies of the BioSupraMol core facility.
Contacts: Dr. Boris Schade (boris.schade@fzem.fu-berlin.de)
Affiliated Communities: TEM users, ACEM
Comments:
Service to request assisted and unassisted TEM measurements at the FZEM. Approvals of the request by your group head may be required.
Provider UsagePolicy: https://www.biosupramol.de/Ressourcen/Dokumente/BSM_Nutzerregelung.pdf
Provider Contact: kai.ludwig@fzem.fu-berlin.de
Provider Location: Fabeckstraße 36A, 14195 Berlin, Germany
Name: STEM Nion HERMES 200 kV
Resource type: Transmission Electron Microscope
Provider: BeAM - Berlin-Adlershof Center for Advanced Microscopy
Affiliations: HU Berlin, FHI, HZB, IKZ
Description:
- monochromated (energy resolution < 6 meV)
- aberration-corrected (probe size at 200 kV < 0.07 nm)
- side entry stage (double-tilt cryo holder with electrical contacts, etc.)
- direct detector for EELS and zero-loss filtered diffraction (Dectris)
- sample in ultra-high vacuum
Contacts: benedikt.haas@physik.hu-berlin.de
Affiliated Communities: ACEM
Provider Location: Berlin
Name: TEM/STEM JEOL JEM2200FS
Resource type: Transmission Electron Microscope
Provider: BeAM - Berlin-Adlershof Center for Advanced Microscopy
Affiliations: HU Berlin, FHI, HZB, IKZ
Description:
- 200 kV, field emission gun
- UHR pole piece
- 1k slow-scan CCD camera (Gatan)
- ASTAR system (NanoMegas) including Beam precession unit, SNBD system, ACOM software
- BF STEM detector
- HAADF STEM detector
- Electron biprism
- Digiscan system (Gatan)
- In-column energy filter
- LN2 free energy dispersive X-ray SD detector (Bruker)
Contacts: holm.kirmse@physik.hu-berlin.de
Affiliated Communities: ACEM
Provider Location: Berlin
Name: Course on human induced pluripotent Stem Cells (iPSCs) - 20-22 November 2024
Resource type: Training
Provider: BIH Core Unit pluripotent Stem Cells & Organoids
Affiliations: Charité, BIH
Description:
Working with human pluripotent stem cells (hPSC), including generation, differentiation as well as establishment of disease models requires a profound knowledge and hands-on expertise. The processes involved in these aspects of hPSC handling in tissue culture are labor intensive and distinct from that of other mammalian cell cultures. The BIH Core Unit pluripotent Stem Cells & Organoids (CUSCO) and the MDC Technology Platform pluripotent Stem Cells are jointly offering training on these methodologies.
The lectures and practical training will cover all areas of hPSC technology including techniques and practical advice for maintenance, characterization & QC, differentiation and genetic manipulation.
The lectures and lab training will be at the MDC in Berlin Buch!
When?
20-22. November 2024
Who should attend this course?
The training is intended for researchers and technicians who are planning projects using hPSCs and/or will start working with hPSCs in the near future.
The hands-on part of the training is limited to six attendees to provide a comfortable learning experience and allow for personalized interactions between the trainers and attendees. The lectures are limited to 20 max attendees.
Prerequisite
Previous hands-on experience in mammalian cell culture, familiarity with basic molecular and cell biology principles and methods are mandatory. The course is open to BIH, Charité and MDC investigators.
Registration
Please apply until 10. November 2024.
The course is free of charge!
For any questions, please contact Dr. Sebastian Diecke (Sebastian.diecke@mdc-berlin.de) or Dr. Harald Stachelscheid (harald.stachelscheid@bih-charite.de).
Contacts:
Comments:
20-22 November 2024
Provider UsagePolicy:
Terms of Service
BIH Core Unit pluripotent Stem Cells and Organoids
Valid from 24.05.2024
These Terms govern your access to and use of BIH Core Unit pluripotent Stem Cells and Organoids (CUSCO) services and equipment. Our Services are offered subject to your acceptance, without modification, of all the terms and conditions contained herein and all other operating rules, and procedures that may be published from time to time by us. You agree that we may automatically upgrade our Services, and the Agreement will apply to any upgrades. Please read the Agreement carefully before accessing or using our Services. By accessing or using any part of our Services, you agree to become bound by the Agreement. If you do not agree to all the terms of the Agreement, then you may not access or use our Services.
Your Account
The use of our Services requires an account in the openIRIS system (https://iris.charite.de), hence, you agree to provide us with complete and accurate information when you register for an account and keep the information current. This is important because sometimes we may need to send you notable updates. You will be solely responsible and liable for any activity that occurs under your account. You are responsible for keeping your account information up-to-date and for keeping your password secure. You are responsible for maintaining the security of your account and you are fully responsible for all activities that occur under your account and any other actions taken in connection with our Services. You shall not share or misuse your access credentials. You must immediately notify us of any unauthorized uses of your account or of any other breach of security. We will not be liable for any acts or omissions by you, including any damages of any kind incurred as a result ofsuch acts or omissions. Your account will be active for one year and can then be prolonged.
In addition, the following must be observed before the account is created:
- Before use, a free consultation must take place to assess the need and scope.
- Project-related support must be submitted using a project registration in openIRIS and include project title, description of the expected services, and the client.
- Access to the Infrastructure and Services of CUSCO is approved only to the Users who have registered their projects and themselves as USERS via openIRIS system and receive the specific introduction by the CUSCO personnel.
Following account activation, you as a CUSCO user, agree to adhere to the following obligations:
- with the regulations of the user rules and the specific laboratory and equipment rules (see Service and Equipment).
- to attend the annual safety instructions for the necessary laboratory safety levels.
- to strictly follow the instructions of the CUSCO staff in the CUSCO rooms and when using the equipment.
- to make the CUSCO staff aware of the existence of safety risks related to the experimental material (e.g., pathogenic, infectious, toxic, or radioactive properties or according to Biostoffverordnung (BioStoffV)).
- if necessary, to provide evidence of corresponding notifications and approvals (here in particular the approval of the genetic engineering experiments to be carried out or experiments on animals or humans, if necessary, with the approval of the ethics committee) of research projects and experiments. Patient data are to be handled by the user in compliance with the relevant data protection regulations and are to be handed over to CUSCO exclusively in anonymized form.
- any work with genetically modified organisms (according to Gentechnikgesetz (GenTG)) is only allowed after a prior approval with the CUSCO director.
- if necessary, authorize CUSCO to perform the experiments.
- Operate the equipment and perform the experiments only after the instruction of CUSCO staff.
Service and equipment
Within the framework of its available capacities, the CUSCO performs the following services in particular:
- Project consultation and support
- Isolation of primary cells from patient material
- Reprogramming of cells into human induced pluripotent stem cells (hiPSCs)
- Banking, characterization, and quality control of hPSCs
- Genome editing using CRISPR/Cas
- Establishment of differentiation protocols
- Provision of cells differentiated from hiPSCs
- Establishment of methods for organoid generation and analysis
- Provision of hiPSC reference cell lines
- Provision of reagents
- Provision of infrastructure and equipment
- Training
In addition, CUSCO manages access to and supports, the shared equipment (specific equipment available is listed in openIRIS).
Equipment managed by CUSCO is available to all CUSCO registered users. By accepting these Terms of Service, the users agree to adhere to the following rules of the equipment use:
- Equipment access and training must be requested through openIRIS. Approval of access will be granted only to the users that completed the training.
- All equipment available at CUSCO must be booked through openIRIS before use. Use of equipment without booking is strictly forbidden.
- Equipment booking expires 30 min after the booking time has begun if the user is not present, but usage fees will apply.
- In case the use time exceeds the booking time, additional time must be booked in openIRIS.
- Bookings can be made up to a maximum of 14 days before planned use. Cancellation of a booking can be made up to 24 hours before the start of the booked period. Cancellations made after this time may result in usage fees if no other user books this period.
- Note that repeated absence during booked time, use of device without booking and/or misuse of equipment will result in user access cancellation.
- Users are responsible for immediate removal of data from the CUSCO computers. CUSCO is not responsible for data storage and safety. All data older than four weeks will be automatically deleted from the PCs without further notice.
- It is not allowed to store any kind of data anywhere else on the CUSCO computers other than in the respective directories. All data in other locations (for example on the desktop) will be deleted without further notice.
- Equipment manuals and accessories must remain with the respective device at any time (PDFs of manuals can be provided on request by CUSCO).
- In case of a device malfunction, the CUSCO personnel must be immediately informed. Error messages must be copied or documented by a screenshot and uploaded as an “issue” through openIRIS.
- Cells provided to the user must not be shared with other users or researchers outside of the core facility or without consent of the head of CUSCO.
Liability
All users are liable according to the legal regulations. This applies in particular to damage caused by failure to comply with the obligations incumbent on the users, by non-clarification of security risks or by non-compliance with binding instructions issued by the staff. The responsibility for the quality of the incoming material or incoming data of a project lies with the user.
The liability of CUSCO towards users is limited to intent and gross negligence. The CUSCO do not assume any warranty for the test material.
Genetically modifies organisms (GMO)
Regulations regarding working with and handling genetically modified organisms or materials containing genetically modified organisms are described in the Federal Republic of Germany by the Genetic Engineering Act (Gentechnikgesetz-GenTG).
All users requesting genetically modified material or genetic modification of iPSCs (Service: genetic modification) and/or bringing and working with genetically modified organisms in the CUSCO facility are required to inform the CUSCO personnel about such work and provide a copy of the official permission and registration of the genetic engineering facility / laboratory.
For Charité internal regulations see: https://intranet.charite.de/biosicherheit/startseite/
Publications
Users are obliged to take due account of the work of CUSCO in publications following the generally accepted rules of scientific practice:
- In principle, any external work, e.g., the work of CUSCO, must be identified at the appropriate points in scientific work, e.g., in the material and method part. The payment of fees or charges resulting from service does not replace the corresponding labelling of technical or scientific work.
- Depending on the scope and complexity of the work or in the case of corresponding collaboration agreements, the CUSCO or individual employees must be considered by mentioning them in the acknowledgment[1] or within the framework of a co-authorship. Co-authorship of CUSCO scientists usually requires a scientific contribution that goes far beyond the mere performance of experiments or analyses.
- The CUSCO should be notified before submission of the publication. If necessary, the corresponding files must be made available for inspection beforehand. Also, a copy of the publication in electronic form must be sent to the head of CF Stem Cells after publication.
Exclusion and restriction of use
In particular, the approval for use may be refused, revoked, or further restricted if
- no proper application has been submitted
- the information in the application is not or is no longer correct
- a fixed usage fee is not paid
- the regulations for use, the specific laboratory and equipment rules or instructions of CUSCO staff are violated
- CUSCO is not mentioned or adequately considered in publications according to chapter „Publications“.
The user is not entitled to claims for damages due to the refusal, revocation, or subsequent limitation of admission.
Service Catalogue / Usage Fees
- The fees to be paid by the users for CUSCO services can be found in the current list of fees. Special rates apply to external users.
- For projects regarding technology or method development, including feasibility studies, within the framework of a cooperation, the prices can be reduced or, in exceptional waived at CUSCO staff discretion. Co-authorships of CUSCO staff are expected to be considered in the resulting publications according to their scientific or technical contributions.
- The user fees are charged after receipt of the services. In the case of internal customers, this is done via internal service charging (interne Leistungsverrechnung, ILV). External users always receive an invoice.
- Project leaders can request user fees for funding applications (in DFG applications under the item „Requested other costs“, CUSCO is registered in the DFG Database). The team of CUSCO will be available to assist you with the appropriate cost estimation during the application phase.
- CUSCO reserves the right to issue a partial charge on the failed services. Due to underlying biological limitations, CUSCO is unable to always guarantee the success of the following services:
- Reprogramming
- Genome editing
- Differentiation
CUSCO staff guarantees to perform necessary experimental steps to fulfil the service request. However, in an unlikely event of the service failure, the number of further attempts will be limited as defined by individual service descriptions and the services will be continued only after an explicit confirmation from the User. The user agrees to the payment of charges according to the steps completed by CUSCO in the attempts to fulfil the service.
è The current list of fees is attached as Appendix 3.
Severability clause and validity of the user regulations
The user regulations are binding for all users of CUSCO. Agreements beyond the scope of these Terms of Service are made with external users and must be issued in writing. The Terms of Service are valid in the respective version without limitation until they are replaced by an updated version.
Contact
Director: Dr. Harald Stachelscheid
Charité – Universitätsmedizin Berlin
BIH Core Unit pluripotent Stem Cells & Organoids
Cranach Haus
Augustenburger Platz 1
13353 Berlin
(Visitors: Föhrer Straße 15)
Email: CUSCO@bih-charite.de
Appendix 1: Specific rules for equipment use
MACSQuant VYB:
- Users must use personal accounts. Account sharing is not allowed.
- Personal account will be assigned immediately after user introduction.
- Calibration of the machine is performed by the CUSCO personnel once a month.
MACSQuant Tyto FACS Sorter:
- Users must use personal accounts. Account sharing is not allowed.
- Workspace set-up and advanced training cannot be provided by CUSCO and must be requested by the user to Miltenyi directly. CUSCO only provides access to the Sorter, an account, and general recommendations for equipment use.
- Accessories, like Tyto magnet for cartridge load, must be used at CUSCO lab. It is strictly forbidden to remove equipment from CUSCO lab space.
- No gloves are permitted when working with the system.
- The computers operating this equipment must not be switched off. It is critical for the instrument operation that the computers stay active.
- Use of the computer for applications other than device control is not allowed.
- The use of USB storage devices is prohibited.
- must be trained in the operation of the unit by an experienced user. After this training, CUSCO staff will evaluate the ability of the user to operate the unit properly and permit access.
- Measurement data must be transferred to the Columbus server (http://columbus.charite.de) or OMERO Server (https://omero.charite.de) immediately after the measurement and removed from the measurement computer.
Leica inverted Microscope (User Lab):
- Data must be saved in a folder named after the group/username in the following location:
“Dieser PC > Dokumente > Folder [Group Name] > [ username]”
- Data must be saved in a folder named after the group/username in the following location:
“D:> cap_0.5.1.4182005e > folder [username]”QuantStudio 6:
- Data must be saved in a folder named after the group/username in the following location: “D:UsersPublicDocumentsQuantStudio Real-Time PCR softwareexperiments[Group Name][user name]”
Opera Phenix, Operetta and Columbus Server
- All image data should be moved immediately after acquisition to the central OMERO Server (https://omero.charite.de) of the Charité
Picking hood Microscope (User Lab):
- Data must be directly saved in external source (USB stick) and/or deleted immediately from the computer hard disk after transfer.
Chromium Controller
- Only the use of the Controller and accessories ad-hoc are allowed (chip holder and vortex with adaptor for beads rack).
- Accessories must be used at CUSCO lab; it is not allowed to move them somewhere else.
Molecular biology work bench rules
- Molecular biology bench must be booked via openIRIS when Chromium Controller is used.
- This bench is reserved for single cell experiments. Cryostat
- It is mandatory to clean up the device after use with ethanol 80% as it was instructed during the training. Remnants of embedding medium can damage the device.
Leica LSM SP8
- Use of the computer for applications other than device control is not permitted.
- The use of USB storage devices is prohibited.
- All image data should be moved immediately after acquisition to the central OMERO Server (https://omero.charite.de) of the Charité
INCUCYTE – User rules
- It is mandatory to receive training from CUSCO. Training can be booked using OpenIris.
- Only the use of a personal account is allowed.
- Incucyte software can be downloaded in every Charité PC (instructions will be provided during the training). If needed the Working station (ground floor, room 0.0134) can be used to schedule experiments. It is not permitted to use other CUSCO computers.
- Incucyte use is allowed only during booked time slots. Booking has to be scheduled using OpenIris.
- Booking is mandatory for the whole time of experiment/the plates are in the Incucyte.
- ONly 2 concurrent bookings are possible. Each booking allows the use of 2 (out of 6) Incucyte plate-positions (i.e. 1 user = 1 booking slot = 2 positions of the tray).
- 2 (out of 6) spots of the tray are reserved for single scannings (“Scan now” acquisition type). Therefore, those 2 spots HAVE to be free and only used during the single scan (limited time - single time).
- Each booking (up to 2 plates) can use a schedule of a maximum of 30 minutes duration per hour.
- It is mandatory to leave every 3 hours 1 slot of 30 minutes free for spontaneous measurements
- Do book your slots time ahead (preferably 1 week) to avoid undesirable overlapping between users. Booking can be cancelled until 1 hour before it starts (please contact CUSCO member if needed). Booking cancel within 1 hour prior starting will be charged.
- Plate loading has to be done carefully and holding the tray from below.
- You HAVE to remove your plate after the experiment has finished and delete the acquisition schedule from the Software to allow next measurement. If plate is abandoned (no openIRIS booking visible), CUSCO will discard it without further notice.
- If you notice before starting your experiment that water in the incubator is missing or any other anomaly (not signaled by alarm) please contact a CUSCO member.
- Data will be deleted every 2 months. During this time you can analyze and archive your data after downloading.
- Data export to the ImageArtist server is possible. Please contact CUSCO member to learn how to proceed.
- Do not set for acquisition empty wells, focus will not be found and the whole scanning may stop.
Appendix 2: Specific rules for use Cell culture labs
- It is mandatory to book cell culture safety cabinet in openIRIS before use. In case the use time exceeds the booking time, additional time must be booked in openIRIS.
- Use of PPE (lab coat and gloves) is mandatory in the cell culture labs.
- User responsibilities:
è Refill general consumables, for example: 80% ethanol, paper tissues, glass pipettes, plastic material, etc.
è Dispose of trash as indicated during lab introduction (white bins, yellow glass containers, already full trash bags).
è Empty liquid waste (vacuum container).
è Inform CUSCO personnel if reagents or consumables are running out (use the white board outside of the cell culture room to leave notices). Provide enough time before the material is completely consumed.
è Inform CUSCO personnel if any issue with equipment or space is encountered.
è Keep safety cabinets, incubators, water bath and general space clean.
è Re-fill water in incubator if needed using deionized autoclaved water (red cap bottles).
è Only pipette tips with filter are allowed in the cell culture safety cabinets. Please store only one box of pipette tips per size for each bench and make sure they are closed properly when removed from the safety cabinet.
- It is not allowed to stock a large number of aliquots of reagents from the user projects in the fridge/freezer of the user lab. Keep only what is needed in the space assign to you for a month. Space is limited.
- All reagents, plates, boxes, etc. must be properly and clearly labelled. The label must include: the Group, Initials and Date. CUSCO personnel will discard all unidentifiable material without further notice.
- It is not allowed to store samples (DNA, RNA, cell pellets, imaging plates, etc.) in fridge/freezer of the user lab for long term. When a user is not working in the facility anymore (not active) and after notification, we will remove and discard all reagents/samples of the user. Do not store any belongings on the benches/cupboards, they will be removed. It is a common working area.
- After completion of work:
- Clean bench and disinfect the surface with 80% ethanol!
- Clean the tube of the pump first with deionized water and subsequently with 80% ethanol after usage
- Switch off lamps of the microscopes according to the introduction!
- If necessary (last user in the cell culture lab), switch off the bench, pump, water bath and microscope.
- The following materials will be provided by CUSCO and are included in the bench usage fee:
- Serological Pipettes (5ml, 10ml and 25ml)
- Falcon Tubes (15ml and 50ml)
- Plates (6-wells and 12-wells)
- Filter Tips
- Ethanol
- Yellow waste bins
- Gloves
- EDTA solution
- Eppendorf tubes
Furthermore, the following reagents are provided but must be purchased in openIRIS prior use:
- Vitronectin
- ROCK inhibitor
- mTeSR medium
- E8 medium
Appendix 3:Price list of CUSCO internal users (billing via ILV)
| Description | Unit | Price [€] |
|---|---|---|
| Generation of iPSCs, Banking and Characterisation | ||
| Primary cell Isolation | Donor | 90,00 |
| Derivation of iPSCs from primary cells | Donor | 3550,00 |
| Banking of iPS cell line (48 vials) | cell line | 640,00 |
| QC and Characterisation - basic Panel | cell line | 1230,00 |
| Cell Line Identity (STR Analysis) | cell line | 150,00 |
| Virtual Karyotyping using a SNP Array | cell line | 280,00 |
| G-Banding Karyotyping | cell line | 450,00 |
| Morphology and Viability | cell line | 290,00 |
| Sterility and Mycoplasma testing | cell line | 70,00 |
| Marker for undifferentiated iPSCs (IF) | cell line | 340,00 |
| Marker for undifferentiated iPSCs (FACS) | cell line | 400,00 |
| Differentiation capacity of hPSCs | cell line | 640,00 |
| Confirmation of gene editing | cell line | 80,00 |
| Virologic testing of cultured cells | cell line | 510,00 |
| Bank of single cell culture adapted hPSC | cell line | 1710,00 |
| Differentiated Cells and Organoids | ||
| Generation of endothelial cells | BATCH | 1250,00 |
| Generation of hepatocytes | PLATE | 720,00 |
| Generation of neural stem cells | BATCH | 1390,00 |
| Generation of iNeurons (NGN2 induced) | BATCH | 480,00 |
| Celebral organoids - StemCellTech Kit | BATCH | 1460,00 |
| Generation of human Lung Organoids (hLOA) | BATCH | 980,00 |
| Passaging and expansion of hLOA | BATCH | 200,00 |
| Generation of human Intestinal Organoids (hIO) | BATCH | 580,00 |
| Passaging and expansion of hIO | BATCH | 420,00 |
| Gene editing and complex projects | ||
| Supervision | hour | 41,00 |
| CRISPR Design | cell line | 210,00 |
| gRNA Efficiency testing | cell line | 650,00 |
| CRISPR experiment | cell line | 530,00 |
| Single cell cloning with IOTA | cell line | 1000,00 |
| Clone Validation | cell line | 1380,00 |
| Provision of reagents | ||
| E8 Media (500ml) - own production | BOTTLE | 84,00 |
| E8 Media (500ml) - own production KIT | BOTTLE | 84,00 |
| mTeSR Media (500ml) - own production | BOTTLE | 121,00 |
| mTeSR Media (500ml) | BOTTLE | 395,00 |
| Geltrex Aliquots (10 ml) | Aliquot | 82,00 |
| Vitronectin Aliquots (600 µl) | Aliquot | 81,00 |
| ROCK Inhibitor Y-27632 (1 ml) | Aliquot | 68,00 |
| Provision of cells | ||
| Provision of live iPSCs | WELL | 21,00 |
| Cryopreserved iPCSs | VIAL | 79,00 |
| Cryopreserved iPCSs - own bank | VIAL | 15,00 |
| Cryopreserved Neuronal Stem Cells | VIAL | 30,00 |
| Cryopreserved Endothelial Cells | VIAL | 44,00 |
| Devices | ||
| FACS Analyzer MACS Quant VYB | hour | 13,00 |
| FACS Sorter MACS Quant Tyto | hour | 26,00 |
| Real-time PCR Quantstudio6 | hour | 8,00 |
| 10X Chromium Controller | hour | 63,00 |
| Leica Confocal SP8 | hour | 13,00 |
| Opera Phenix High Content Screener | hour | 16,00 |
| Opera Phenix High Content Screener-off h | hour | 5,00 |
| IsoCell | hour | 27,00 |
| TapeStation | hour | 8,00 |
| Incucyte SX5 | hour | 3,00 |
| Cell3Imager Duos | hour | 13,00 |
| Bench fee - User-Lab | hour | 10,00 |
| Training | ||
| Equipment training | hour | 47,00 |
| Hands-on training - Basic hPSC culture | training | 620,00 |
| Hands-on training - iNeurons | training | 530,00 |
| Hands-on training - NSCOs | training | 320,00 |
| Hands-on training - hLOA generation | training | 380,00 |
| Hands-on training - hIO generation | training | 500,00 |
| Hands-on training - Endothelial cells | training | 780,00 |
[1] Please refer to the following formulation example: „This work was supported by the Core Unit pluripotent Stem Cells and Organoids of the Berlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin.”
Provider Location: Charité Campus Virchow-Klinikum (CVK), Berlin
Name: Oxford Diffraction Xcalibur Nova O
Resource type: X-ray Diffractometer
Provider: X-Ray diffraction unit - BCP
Affiliations: FU Berlin
Description: This Oxford Diffraction Xcalibur Nova O single crystal diffractometer is available for non-assisted measurements for trained persons from FUB. It is equipped a Cu x-ray sources, an Onyx CCD detector, and an Oxford CryojetXL.
Contacts: Dr. Anja Wiesner (anja.wiesner@fu-berlin.de)
Comments: Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible in three predetermined blocks.
Provider Contact: anja.wiesner@fu-berlin.de
Provider Location: Fabeckstraße 34-36, 14195 Berlin
Name: Project consultation
Resource type: Consultation
Provider: BIH Core Unit pluripotent Stem Cells & Organoids
Affiliations: Charité, BIH
Description:
To allow sufficient time for all our users and be able to continue developing Core Projects we ask that ALL the meetings to be scheduled in advance through this service request. Please send us a request for a meeting indicating who you want to meet and with suggested time and we will respond promptly with our availability.
These include:
- Experiment/Project planning
- Help with experiment execution/ consultation
- Equipment help
- Updates/results discussions
For questions and requests regarding consumables (media, plasticware, chemicals, etc.), cell lines and standard protocols, please contact the technicians.
Contacts: Harald Stachelscheid
Provider UsagePolicy:
Terms of Service
BIH Core Unit pluripotent Stem Cells and Organoids
Valid from 24.05.2024
These Terms govern your access to and use of BIH Core Unit pluripotent Stem Cells and Organoids (CUSCO) services and equipment. Our Services are offered subject to your acceptance, without modification, of all the terms and conditions contained herein and all other operating rules, and procedures that may be published from time to time by us. You agree that we may automatically upgrade our Services, and the Agreement will apply to any upgrades. Please read the Agreement carefully before accessing or using our Services. By accessing or using any part of our Services, you agree to become bound by the Agreement. If you do not agree to all the terms of the Agreement, then you may not access or use our Services.
Your Account
The use of our Services requires an account in the openIRIS system (https://iris.charite.de), hence, you agree to provide us with complete and accurate information when you register for an account and keep the information current. This is important because sometimes we may need to send you notable updates. You will be solely responsible and liable for any activity that occurs under your account. You are responsible for keeping your account information up-to-date and for keeping your password secure. You are responsible for maintaining the security of your account and you are fully responsible for all activities that occur under your account and any other actions taken in connection with our Services. You shall not share or misuse your access credentials. You must immediately notify us of any unauthorized uses of your account or of any other breach of security. We will not be liable for any acts or omissions by you, including any damages of any kind incurred as a result ofsuch acts or omissions. Your account will be active for one year and can then be prolonged.
In addition, the following must be observed before the account is created:
- Before use, a free consultation must take place to assess the need and scope.
- Project-related support must be submitted using a project registration in openIRIS and include project title, description of the expected services, and the client.
- Access to the Infrastructure and Services of CUSCO is approved only to the Users who have registered their projects and themselves as USERS via openIRIS system and receive the specific introduction by the CUSCO personnel.
Following account activation, you as a CUSCO user, agree to adhere to the following obligations:
- with the regulations of the user rules and the specific laboratory and equipment rules (see Service and Equipment).
- to attend the annual safety instructions for the necessary laboratory safety levels.
- to strictly follow the instructions of the CUSCO staff in the CUSCO rooms and when using the equipment.
- to make the CUSCO staff aware of the existence of safety risks related to the experimental material (e.g., pathogenic, infectious, toxic, or radioactive properties or according to Biostoffverordnung (BioStoffV)).
- if necessary, to provide evidence of corresponding notifications and approvals (here in particular the approval of the genetic engineering experiments to be carried out or experiments on animals or humans, if necessary, with the approval of the ethics committee) of research projects and experiments. Patient data are to be handled by the user in compliance with the relevant data protection regulations and are to be handed over to CUSCO exclusively in anonymized form.
- any work with genetically modified organisms (according to Gentechnikgesetz (GenTG)) is only allowed after a prior approval with the CUSCO director.
- if necessary, authorize CUSCO to perform the experiments.
- Operate the equipment and perform the experiments only after the instruction of CUSCO staff.
Service and equipment
Within the framework of its available capacities, the CUSCO performs the following services in particular:
- Project consultation and support
- Isolation of primary cells from patient material
- Reprogramming of cells into human induced pluripotent stem cells (hiPSCs)
- Banking, characterization, and quality control of hPSCs
- Genome editing using CRISPR/Cas
- Establishment of differentiation protocols
- Provision of cells differentiated from hiPSCs
- Establishment of methods for organoid generation and analysis
- Provision of hiPSC reference cell lines
- Provision of reagents
- Provision of infrastructure and equipment
- Training
In addition, CUSCO manages access to and supports, the shared equipment (specific equipment available is listed in openIRIS).
Equipment managed by CUSCO is available to all CUSCO registered users. By accepting these Terms of Service, the users agree to adhere to the following rules of the equipment use:
- Equipment access and training must be requested through openIRIS. Approval of access will be granted only to the users that completed the training.
- All equipment available at CUSCO must be booked through openIRIS before use. Use of equipment without booking is strictly forbidden.
- Equipment booking expires 30 min after the booking time has begun if the user is not present, but usage fees will apply.
- In case the use time exceeds the booking time, additional time must be booked in openIRIS.
- Bookings can be made up to a maximum of 14 days before planned use. Cancellation of a booking can be made up to 24 hours before the start of the booked period. Cancellations made after this time may result in usage fees if no other user books this period.
- Note that repeated absence during booked time, use of device without booking and/or misuse of equipment will result in user access cancellation.
- Users are responsible for immediate removal of data from the CUSCO computers. CUSCO is not responsible for data storage and safety. All data older than four weeks will be automatically deleted from the PCs without further notice.
- It is not allowed to store any kind of data anywhere else on the CUSCO computers other than in the respective directories. All data in other locations (for example on the desktop) will be deleted without further notice.
- Equipment manuals and accessories must remain with the respective device at any time (PDFs of manuals can be provided on request by CUSCO).
- In case of a device malfunction, the CUSCO personnel must be immediately informed. Error messages must be copied or documented by a screenshot and uploaded as an “issue” through openIRIS.
- Cells provided to the user must not be shared with other users or researchers outside of the core facility or without consent of the head of CUSCO.
Liability
All users are liable according to the legal regulations. This applies in particular to damage caused by failure to comply with the obligations incumbent on the users, by non-clarification of security risks or by non-compliance with binding instructions issued by the staff. The responsibility for the quality of the incoming material or incoming data of a project lies with the user.
The liability of CUSCO towards users is limited to intent and gross negligence. The CUSCO do not assume any warranty for the test material.
Genetically modifies organisms (GMO)
Regulations regarding working with and handling genetically modified organisms or materials containing genetically modified organisms are described in the Federal Republic of Germany by the Genetic Engineering Act (Gentechnikgesetz-GenTG).
All users requesting genetically modified material or genetic modification of iPSCs (Service: genetic modification) and/or bringing and working with genetically modified organisms in the CUSCO facility are required to inform the CUSCO personnel about such work and provide a copy of the official permission and registration of the genetic engineering facility / laboratory.
For Charité internal regulations see: https://intranet.charite.de/biosicherheit/startseite/
Publications
Users are obliged to take due account of the work of CUSCO in publications following the generally accepted rules of scientific practice:
- In principle, any external work, e.g., the work of CUSCO, must be identified at the appropriate points in scientific work, e.g., in the material and method part. The payment of fees or charges resulting from service does not replace the corresponding labelling of technical or scientific work.
- Depending on the scope and complexity of the work or in the case of corresponding collaboration agreements, the CUSCO or individual employees must be considered by mentioning them in the acknowledgment[1] or within the framework of a co-authorship. Co-authorship of CUSCO scientists usually requires a scientific contribution that goes far beyond the mere performance of experiments or analyses.
- The CUSCO should be notified before submission of the publication. If necessary, the corresponding files must be made available for inspection beforehand. Also, a copy of the publication in electronic form must be sent to the head of CF Stem Cells after publication.
Exclusion and restriction of use
In particular, the approval for use may be refused, revoked, or further restricted if
- no proper application has been submitted
- the information in the application is not or is no longer correct
- a fixed usage fee is not paid
- the regulations for use, the specific laboratory and equipment rules or instructions of CUSCO staff are violated
- CUSCO is not mentioned or adequately considered in publications according to chapter „Publications“.
The user is not entitled to claims for damages due to the refusal, revocation, or subsequent limitation of admission.
Service Catalogue / Usage Fees
- The fees to be paid by the users for CUSCO services can be found in the current list of fees. Special rates apply to external users.
- For projects regarding technology or method development, including feasibility studies, within the framework of a cooperation, the prices can be reduced or, in exceptional waived at CUSCO staff discretion. Co-authorships of CUSCO staff are expected to be considered in the resulting publications according to their scientific or technical contributions.
- The user fees are charged after receipt of the services. In the case of internal customers, this is done via internal service charging (interne Leistungsverrechnung, ILV). External users always receive an invoice.
- Project leaders can request user fees for funding applications (in DFG applications under the item „Requested other costs“, CUSCO is registered in the DFG Database). The team of CUSCO will be available to assist you with the appropriate cost estimation during the application phase.
- CUSCO reserves the right to issue a partial charge on the failed services. Due to underlying biological limitations, CUSCO is unable to always guarantee the success of the following services:
- Reprogramming
- Genome editing
- Differentiation
CUSCO staff guarantees to perform necessary experimental steps to fulfil the service request. However, in an unlikely event of the service failure, the number of further attempts will be limited as defined by individual service descriptions and the services will be continued only after an explicit confirmation from the User. The user agrees to the payment of charges according to the steps completed by CUSCO in the attempts to fulfil the service.
è The current list of fees is attached as Appendix 3.
Severability clause and validity of the user regulations
The user regulations are binding for all users of CUSCO. Agreements beyond the scope of these Terms of Service are made with external users and must be issued in writing. The Terms of Service are valid in the respective version without limitation until they are replaced by an updated version.
Contact
Director: Dr. Harald Stachelscheid
Charité – Universitätsmedizin Berlin
BIH Core Unit pluripotent Stem Cells & Organoids
Cranach Haus
Augustenburger Platz 1
13353 Berlin
(Visitors: Föhrer Straße 15)
Email: CUSCO@bih-charite.de
Appendix 1: Specific rules for equipment use
MACSQuant VYB:
- Users must use personal accounts. Account sharing is not allowed.
- Personal account will be assigned immediately after user introduction.
- Calibration of the machine is performed by the CUSCO personnel once a month.
MACSQuant Tyto FACS Sorter:
- Users must use personal accounts. Account sharing is not allowed.
- Workspace set-up and advanced training cannot be provided by CUSCO and must be requested by the user to Miltenyi directly. CUSCO only provides access to the Sorter, an account, and general recommendations for equipment use.
- Accessories, like Tyto magnet for cartridge load, must be used at CUSCO lab. It is strictly forbidden to remove equipment from CUSCO lab space.
- No gloves are permitted when working with the system.
- The computers operating this equipment must not be switched off. It is critical for the instrument operation that the computers stay active.
- Use of the computer for applications other than device control is not allowed.
- The use of USB storage devices is prohibited.
- must be trained in the operation of the unit by an experienced user. After this training, CUSCO staff will evaluate the ability of the user to operate the unit properly and permit access.
- Measurement data must be transferred to the Columbus server (http://columbus.charite.de) or OMERO Server (https://omero.charite.de) immediately after the measurement and removed from the measurement computer.
Leica inverted Microscope (User Lab):
- Data must be saved in a folder named after the group/username in the following location:
“Dieser PC > Dokumente > Folder [Group Name] > [ username]”
- Data must be saved in a folder named after the group/username in the following location:
“D:> cap_0.5.1.4182005e > folder [username]”QuantStudio 6:
- Data must be saved in a folder named after the group/username in the following location: “D:UsersPublicDocumentsQuantStudio Real-Time PCR softwareexperiments[Group Name][user name]”
Opera Phenix, Operetta and Columbus Server
- All image data should be moved immediately after acquisition to the central OMERO Server (https://omero.charite.de) of the Charité
Picking hood Microscope (User Lab):
- Data must be directly saved in external source (USB stick) and/or deleted immediately from the computer hard disk after transfer.
Chromium Controller
- Only the use of the Controller and accessories ad-hoc are allowed (chip holder and vortex with adaptor for beads rack).
- Accessories must be used at CUSCO lab; it is not allowed to move them somewhere else.
Molecular biology work bench rules
- Molecular biology bench must be booked via openIRIS when Chromium Controller is used.
- This bench is reserved for single cell experiments. Cryostat
- It is mandatory to clean up the device after use with ethanol 80% as it was instructed during the training. Remnants of embedding medium can damage the device.
Leica LSM SP8
- Use of the computer for applications other than device control is not permitted.
- The use of USB storage devices is prohibited.
- All image data should be moved immediately after acquisition to the central OMERO Server (https://omero.charite.de) of the Charité
INCUCYTE – User rules
- It is mandatory to receive training from CUSCO. Training can be booked using OpenIris.
- Only the use of a personal account is allowed.
- Incucyte software can be downloaded in every Charité PC (instructions will be provided during the training). If needed the Working station (ground floor, room 0.0134) can be used to schedule experiments. It is not permitted to use other CUSCO computers.
- Incucyte use is allowed only during booked time slots. Booking has to be scheduled using OpenIris.
- Booking is mandatory for the whole time of experiment/the plates are in the Incucyte.
- ONly 2 concurrent bookings are possible. Each booking allows the use of 2 (out of 6) Incucyte plate-positions (i.e. 1 user = 1 booking slot = 2 positions of the tray).
- 2 (out of 6) spots of the tray are reserved for single scannings (“Scan now” acquisition type). Therefore, those 2 spots HAVE to be free and only used during the single scan (limited time - single time).
- Each booking (up to 2 plates) can use a schedule of a maximum of 30 minutes duration per hour.
- It is mandatory to leave every 3 hours 1 slot of 30 minutes free for spontaneous measurements
- Do book your slots time ahead (preferably 1 week) to avoid undesirable overlapping between users. Booking can be cancelled until 1 hour before it starts (please contact CUSCO member if needed). Booking cancel within 1 hour prior starting will be charged.
- Plate loading has to be done carefully and holding the tray from below.
- You HAVE to remove your plate after the experiment has finished and delete the acquisition schedule from the Software to allow next measurement. If plate is abandoned (no openIRIS booking visible), CUSCO will discard it without further notice.
- If you notice before starting your experiment that water in the incubator is missing or any other anomaly (not signaled by alarm) please contact a CUSCO member.
- Data will be deleted every 2 months. During this time you can analyze and archive your data after downloading.
- Data export to the ImageArtist server is possible. Please contact CUSCO member to learn how to proceed.
- Do not set for acquisition empty wells, focus will not be found and the whole scanning may stop.
Appendix 2: Specific rules for use Cell culture labs
- It is mandatory to book cell culture safety cabinet in openIRIS before use. In case the use time exceeds the booking time, additional time must be booked in openIRIS.
- Use of PPE (lab coat and gloves) is mandatory in the cell culture labs.
- User responsibilities:
è Refill general consumables, for example: 80% ethanol, paper tissues, glass pipettes, plastic material, etc.
è Dispose of trash as indicated during lab introduction (white bins, yellow glass containers, already full trash bags).
è Empty liquid waste (vacuum container).
è Inform CUSCO personnel if reagents or consumables are running out (use the white board outside of the cell culture room to leave notices). Provide enough time before the material is completely consumed.
è Inform CUSCO personnel if any issue with equipment or space is encountered.
è Keep safety cabinets, incubators, water bath and general space clean.
è Re-fill water in incubator if needed using deionized autoclaved water (red cap bottles).
è Only pipette tips with filter are allowed in the cell culture safety cabinets. Please store only one box of pipette tips per size for each bench and make sure they are closed properly when removed from the safety cabinet.
- It is not allowed to stock a large number of aliquots of reagents from the user projects in the fridge/freezer of the user lab. Keep only what is needed in the space assign to you for a month. Space is limited.
- All reagents, plates, boxes, etc. must be properly and clearly labelled. The label must include: the Group, Initials and Date. CUSCO personnel will discard all unidentifiable material without further notice.
- It is not allowed to store samples (DNA, RNA, cell pellets, imaging plates, etc.) in fridge/freezer of the user lab for long term. When a user is not working in the facility anymore (not active) and after notification, we will remove and discard all reagents/samples of the user. Do not store any belongings on the benches/cupboards, they will be removed. It is a common working area.
- After completion of work:
- Clean bench and disinfect the surface with 80% ethanol!
- Clean the tube of the pump first with deionized water and subsequently with 80% ethanol after usage
- Switch off lamps of the microscopes according to the introduction!
- If necessary (last user in the cell culture lab), switch off the bench, pump, water bath and microscope.
- The following materials will be provided by CUSCO and are included in the bench usage fee:
- Serological Pipettes (5ml, 10ml and 25ml)
- Falcon Tubes (15ml and 50ml)
- Plates (6-wells and 12-wells)
- Filter Tips
- Ethanol
- Yellow waste bins
- Gloves
- EDTA solution
- Eppendorf tubes
Furthermore, the following reagents are provided but must be purchased in openIRIS prior use:
- Vitronectin
- ROCK inhibitor
- mTeSR medium
- E8 medium
Appendix 3:Price list of CUSCO internal users (billing via ILV)
| Description | Unit | Price [€] |
|---|---|---|
| Generation of iPSCs, Banking and Characterisation | ||
| Primary cell Isolation | Donor | 90,00 |
| Derivation of iPSCs from primary cells | Donor | 3550,00 |
| Banking of iPS cell line (48 vials) | cell line | 640,00 |
| QC and Characterisation - basic Panel | cell line | 1230,00 |
| Cell Line Identity (STR Analysis) | cell line | 150,00 |
| Virtual Karyotyping using a SNP Array | cell line | 280,00 |
| G-Banding Karyotyping | cell line | 450,00 |
| Morphology and Viability | cell line | 290,00 |
| Sterility and Mycoplasma testing | cell line | 70,00 |
| Marker for undifferentiated iPSCs (IF) | cell line | 340,00 |
| Marker for undifferentiated iPSCs (FACS) | cell line | 400,00 |
| Differentiation capacity of hPSCs | cell line | 640,00 |
| Confirmation of gene editing | cell line | 80,00 |
| Virologic testing of cultured cells | cell line | 510,00 |
| Bank of single cell culture adapted hPSC | cell line | 1710,00 |
| Differentiated Cells and Organoids | ||
| Generation of endothelial cells | BATCH | 1250,00 |
| Generation of hepatocytes | PLATE | 720,00 |
| Generation of neural stem cells | BATCH | 1390,00 |
| Generation of iNeurons (NGN2 induced) | BATCH | 480,00 |
| Celebral organoids - StemCellTech Kit | BATCH | 1460,00 |
| Generation of human Lung Organoids (hLOA) | BATCH | 980,00 |
| Passaging and expansion of hLOA | BATCH | 200,00 |
| Generation of human Intestinal Organoids (hIO) | BATCH | 580,00 |
| Passaging and expansion of hIO | BATCH | 420,00 |
| Gene editing and complex projects | ||
| Supervision | hour | 41,00 |
| CRISPR Design | cell line | 210,00 |
| gRNA Efficiency testing | cell line | 650,00 |
| CRISPR experiment | cell line | 530,00 |
| Single cell cloning with IOTA | cell line | 1000,00 |
| Clone Validation | cell line | 1380,00 |
| Provision of reagents | ||
| E8 Media (500ml) - own production | BOTTLE | 84,00 |
| E8 Media (500ml) - own production KIT | BOTTLE | 84,00 |
| mTeSR Media (500ml) - own production | BOTTLE | 121,00 |
| mTeSR Media (500ml) | BOTTLE | 395,00 |
| Geltrex Aliquots (10 ml) | Aliquot | 82,00 |
| Vitronectin Aliquots (600 µl) | Aliquot | 81,00 |
| ROCK Inhibitor Y-27632 (1 ml) | Aliquot | 68,00 |
| Provision of cells | ||
| Provision of live iPSCs | WELL | 21,00 |
| Cryopreserved iPCSs | VIAL | 79,00 |
| Cryopreserved iPCSs - own bank | VIAL | 15,00 |
| Cryopreserved Neuronal Stem Cells | VIAL | 30,00 |
| Cryopreserved Endothelial Cells | VIAL | 44,00 |
| Devices | ||
| FACS Analyzer MACS Quant VYB | hour | 13,00 |
| FACS Sorter MACS Quant Tyto | hour | 26,00 |
| Real-time PCR Quantstudio6 | hour | 8,00 |
| 10X Chromium Controller | hour | 63,00 |
| Leica Confocal SP8 | hour | 13,00 |
| Opera Phenix High Content Screener | hour | 16,00 |
| Opera Phenix High Content Screener-off h | hour | 5,00 |
| IsoCell | hour | 27,00 |
| TapeStation | hour | 8,00 |
| Incucyte SX5 | hour | 3,00 |
| Cell3Imager Duos | hour | 13,00 |
| Bench fee - User-Lab | hour | 10,00 |
| Training | ||
| Equipment training | hour | 47,00 |
| Hands-on training - Basic hPSC culture | training | 620,00 |
| Hands-on training - iNeurons | training | 530,00 |
| Hands-on training - NSCOs | training | 320,00 |
| Hands-on training - hLOA generation | training | 380,00 |
| Hands-on training - hIO generation | training | 500,00 |
| Hands-on training - Endothelial cells | training | 780,00 |
[1] Please refer to the following formulation example: „This work was supported by the Core Unit pluripotent Stem Cells and Organoids of the Berlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin.”
Provider Location: Charité Campus Virchow-Klinikum (CVK), Berlin
Name: Cell Sorter - Aurora CS "Luke" - Full Spectrum Sorter
Resource type: Flow Cytometry Sorter
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description: Full Spectrum Cell Sorter
Contacts: cytometry-core@bih-charite.de
Comments:
Please request a project meeting to get access to cell sorting services.
Please select your slot and wait for approval. Your booking is not guaranteed until you get notification of approval.
Cancel your booking 48 hours ahead to avoid charges.
For ad-hoc, short notice bookings (next 48 hours) please contact us directly.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: Zeiss GeminiSEM 500
Resource type: Scanning Electron Microscope
Provider: BeAM - Berlin-Adlershof Center for Advanced Microscopy
Affiliations: HU Berlin, FHI, HZB, IKZ
Description:
- NanoVP Option
- In-lens SE detector
- In-lens EsB detector
- BSE 4-Quandrant detector
- STEM detector
- Bruker XFlash 6|30 EDX system
- Raith ELPHY Quantum Lithography system
- costum stages for 4D-STEM
Contacts: johannes.mueller@physik.hu-berlin.de
Affiliated Communities: ACEM
Provider Location: Berlin
Name: Aurora "Lane" - Full Spectrum Analyzer
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description:
For more information on full spectrum flow cytometry see:
https://cytekbio.com/blogs/videos#tab-tutorials
https://cytekbio.com/pages/aurora
Contacts: jacqueline.keye@bih-charite.de
Comments:
You need to be registered and trained to use this instrument.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: SR 101 - seminar room (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Small seminar room with tables and 16 seats, equipped with electronic smartboard (mobile). Upgrade with laptop and video camera system is possible. (Webex-)Videomeetings with own laptop possible.
Contacts: Katharina Tebel (ktebel@fu-berlin.de)
Comments: 16 seats, with tables, equipped with electronic smartboard (mobile).
Recurring bookings are possible; please remember to define the end date!
Laptop and video system can be chosen as add-on throughout the booking procedure.
! Bookings become valid only after approval by the staff !
Provider Contact: achim.wiedekind@fu-berlin.de
Provider Location: Freie Universität Berlin
Name: SR 011 - seminar room (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Small seminar room with tables and 16 seats, equipped with electronic smartboard (mobile) and analog whiteboard (fixed). Upgrade with laptop and video camera system is possible. (Webex-)Videomeetings with own laptop possible.
Contacts: Katharina Tebel (ktebel@fu-berlin.de)
Comments: 16 seats, with tables, equipped with electronic smartboard (mobile) and analog whiteboard (fixed).
Recurring bookings are possible; please remember to define the end date!
Laptop and video system can be chosen as add-on throughout the booking procedure.
! Bookings become valid only after approval by the staff !
Provider Contact: achim.wiedekind@fu-berlin.de
Provider Location: Freie Universität Berlin
Name: SR 119 - large seminar room (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Large seminar room without tables, about 100 seats, equipped with a beamer and analog whiteboard (fixed).
Contacts: Katharina Tebel (ktebel@fu-berlin.de)
Comments: 84 seats, without tables, equipped with a beamer and fixed whiteboard (analog).
Recurring bookings are possible; please remember to define the end date!
! Bookings become valid only after approval by the staff !
Provider Contact: achim.wiedekind@fu-berlin.de
Provider Location: Freie Universität Berlin
Name: Small meeting point area / Area in front of kitchen (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Area in front of the large kitchen for small tables / poster area, about 12 couch seats.
Contacts: Katharina Tebel (ktebel@fu-berlin.de)
Comments: 12 couch seats, area in front of large kitchen level 2.
Recurring bookings are possible; please remember to define the end date!
! Bookings become valid only after approval by the staff !
Provider Contact: achim.wiedekind@fu-berlin.de
Provider Location: Freie Universität Berlin
Name: Add my group to FUB IRIS - FU-Berlin
Resource type: Administration (BSM/SFAB - FU)
Provider: Administration OpenIRIS@FUB (BCP)
Affiliations: FU Berlin
Description:
A group is necessary to assign users to a real working group which can adopt the measurement charges. Therefore, only users assigned to a group are able to book resources.
After installation of your group is finished, please do appoint a second group admin that can take over administrative tasks for the group in OpenIRIS.
Contacts: Dr. Boris Schade (boris.schade@fzem.fu-berlin.de)
Comments:
This request addresses to group heads only.
Adding your group to FUB IRIS by this request is restricted to groups of the department of Biology, Chemistry, and Pharmacy (BCP), the department of Physics and the department of Veterinary Medicine.
Provider Contact: boris.schade@fzem.fu-berlin.de
Provider Location: Fabeckstraße 36A, 14195 Berlin
Name: 14. AMBIO Nikon SoRa Spinning Disk Confocal CSU-W1
Resource type: Microscope
Provider: AMBIO
Affiliations: Charité
Description:
Imaging Modes
- Spinning disk confocal (DAPI, CFP, GFP, YFP, mCherry, TxRED, Cy5)
- SoRa-mode (Super Resolution by Optical Pixel Reassignment, 1.4x resolution improvement)
- FRAP/ PA (photo activation/ conversion)
- Widefield EPI fluorescence mode (DAPI, GFP, TxRED, TRITC-B, Cy5)
- DIC for 10x, 40x, 60x, 100x objectives (upon request)
- Live-cell incubator (temperature, CO2, humidity)
7 Laser Lines
- 405 nm
- 445 nm
- 488 nm
- 515 nm
- 561 nm
- 594 nm
- 638 nm
Detectors
- 2x Hamamatsu ORCA-Fusion digital sCMOS cameras (2304x2304 pixels, 100 fps @ 2304x2048 pixels, 80% QE, 6.5 µm pixel size)
- Dual-camera system for simultaneous dual-color detection (e.g. GFP/RFP, RFP/SiR, GFP/SiR, upon request)
Software
- NIS-Elements AR (Advanced Research) including modules for:
- JOBS (automation)
- Deconvolution
- NIS.ai (Artificial Intelligence): denoise, clarify, enhance, segment, convert
Special
- Nipkow CSU-W for large FOV 1024x1024 px [size in µm for 10x]
- Uniform spinning disk illumination
- Enhanced signal-to-noise ratio compared to CSU-X
- 2 cameras for simultaneous dual-color imaging
- Fast emission filter wheel for clean multi-color imaging
- Ultra-fast laser switching for triggered multicolor acquisition
- SoRa-mode: Enhanced resolution (1.4x) in spinning disk mode
- Automatic water immersion dispenser (WID)
Emission Filters
| Filter | λ/Bandwidth |
| Quadband (DAPI, GFP, mCherry, Cy5) | 440/40 521/21 607/34 700/45 |
| DAPI | 447/60 |
| CFP | 472/30 |
| GFP | 525/50 |
| YFP | 542/27 |
| mCherry | 600/50 |
| Cy5 | 685/40 |
Objectives
| Mag. | Type | Immer. | NA | WD [μm] | Info |
| 10x (not installed) | Plan Achroat | Air | 0.45 | 4,000 | DIC N1 λ OFN25 |
| 40x | Apo LWD | Water | 1.15 | 600 | DIC N2 λS |
| 60x | Plan Apo | Water | 1.2 | 310-280 | DIC N2 VC OFN25 |
| 60x | Plan Apo | Oil | 1.4 | 130 | DIC N2 λ OFN25 |
| 25x | Plan Apo | Silicone | 1.05 | 550 | DIC N2 λS OFN25 |
| 40x | Plan Apo | Silicone | 1.25 | 300 | DIC N2 λS OFN25 |
| 100x | Plan Apo | Silicone | 1.35 | 300-290 | SR HP DIC N2 λS OFN22 |
Contacts: ambio@charite.de
Comments: New users - access upon training request here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: SR 201 - big meeting point (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Large seminar room with tables and about 45 seats (more may be possible), equipped with a beamer.
Contacts: Katharina Tebel (ktebel@fu-berlin.de)
Comments: 45 seats (more may be possible), with tables, equipped with a beamer.
Recurring bookings are possible; please remember to define the end date!
! Bookings become valid only after approval by the staff !
Provider Contact: achim.wiedekind@fu-berlin.de
Provider Location: Freie Universität Berlin
Name: 18. AMBIO ZEISS Lattice Lightsheet 7
Resource type: Microscope
Provider: AMBIO
Affiliations: Charité
Description:
Imaging Modes
- Transmitted light with oblique contrast for sample positioning and overview
- Reflected light and epifluorescence with laser for beam adjustment and fluorescence light sheet imaging
- ibidi stage top incubation system (temperature, CO2, humidity) and integrated auto-immersion mechanism enable unattended long-term experiments
- Photo-manipulation: 405 and 473 nm and lasers for FRAP and photo-activation/photo-switch and 780 nm laser for ablation experiments
Acquisition
- Speed: Volume: 3 Vol/s @300 μm x 50 μm x 20 μm, plane: 400 frames/s @300 μm x 20 μm
- Penetration depth up to 80 - 200 μm
Illumination
- LED (white & red) for transmitted light
- Laser for reflected light & epi-fluorescence: 488 nm (2 mW), 561 nm (2 mW), 640 nm (1 mW)
Detectors
- pco.edge 4.2 CLHS sCMOS camera (2048x2048 pixels, 300 x 300 µm, 100 fps@16-bit full frame, 82% QE, 6.5 µm pixel size)
Software
- ZEN 3.5 (blue edition)
- Lattice Lightsheet Processing Module (deskewing, coverglass transformation)
- arivis Vision4D® software for advanced stitching, channel shift, high resolution volume rendering, and powerful deconvolution
Special
- Ultra-fast live 3D subcellular imaging with minimal phototoxicity and bleaching
- Near-isotropic resolution along the X, Y, and Z axes
- Using standard coverslips, 35 mm cell culture imaging dishes or well plates
- 5-axes stage tilt-able with high precision in X and Y
- Automatic water immersion dispenser
Objectives/Optics
- Illumination (light sheet): 13.3x / NA 0.4
- Detection: 44.83x / NA 1.0
Filters
| Filter (λ, Bandwidth) | Sample Fluorophore |
| LBF 405/ 488/ 561/ 642 | All, blocks excitation laser |
| BP 495-550/ BP 570-620 | GFP, mCherry |
| BP 495-550 +LP655 | GFP, Cy5 |
| BP 570-620 +LP655 | mCherry, Cy5 |
| BP 495-590 | GFP, YFP, mCherry |
| EF LP 570 | mCherry, Cy5 |
Contacts: ambio@charite.de
Comments: New users - access upon training request here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: 19. AMBIO ZEISS Lattice Lightsheet 7 Analysis Workstation
Resource type: Workstation
Provider: AMBIO
Affiliations: Charité
Description:
AMBIO ZEISS Lattice Lightsheet 7 Analysis Workstation
Hardware
- CPU: Intel Xeon E5-2630 v4 @ 2x 2.20 GHz, 256 GB RAM
- Graphic: NVIDIA Quadro RTX 6000, GPU Memory 24 GB RAM
- Hard drives: HDD 36 TB, 2x SSD 240 GB
Software
- Windows 10 64-bit System
- ZEISS ZEN 3.5 (blue edition)
- Lattice Lightsheet Processing Module (deskewing, coverglass transformation)
- arivis Vision 4D software mudule for advanced stitching, channel shift, high resolution volume rendering, and powerful deconvolution
- Fiji
Contacts: ambio@charite.de
Comments: Access upon request of analysis project here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: 7th PluriCore Meeting 29 Feb - 1 Mar 2024
Resource type: Event
Provider: BIH Core Unit pluripotent Stem Cells & Organoids
Affiliations: Charité, BIH
Description:
Contacts: Harald.Stachelscheid@bih-charite.de
Provider UsagePolicy:
Terms of Service
BIH Core Unit pluripotent Stem Cells and Organoids
Valid from 24.05.2024
These Terms govern your access to and use of BIH Core Unit pluripotent Stem Cells and Organoids (CUSCO) services and equipment. Our Services are offered subject to your acceptance, without modification, of all the terms and conditions contained herein and all other operating rules, and procedures that may be published from time to time by us. You agree that we may automatically upgrade our Services, and the Agreement will apply to any upgrades. Please read the Agreement carefully before accessing or using our Services. By accessing or using any part of our Services, you agree to become bound by the Agreement. If you do not agree to all the terms of the Agreement, then you may not access or use our Services.
Your Account
The use of our Services requires an account in the openIRIS system (https://iris.charite.de), hence, you agree to provide us with complete and accurate information when you register for an account and keep the information current. This is important because sometimes we may need to send you notable updates. You will be solely responsible and liable for any activity that occurs under your account. You are responsible for keeping your account information up-to-date and for keeping your password secure. You are responsible for maintaining the security of your account and you are fully responsible for all activities that occur under your account and any other actions taken in connection with our Services. You shall not share or misuse your access credentials. You must immediately notify us of any unauthorized uses of your account or of any other breach of security. We will not be liable for any acts or omissions by you, including any damages of any kind incurred as a result ofsuch acts or omissions. Your account will be active for one year and can then be prolonged.
In addition, the following must be observed before the account is created:
- Before use, a free consultation must take place to assess the need and scope.
- Project-related support must be submitted using a project registration in openIRIS and include project title, description of the expected services, and the client.
- Access to the Infrastructure and Services of CUSCO is approved only to the Users who have registered their projects and themselves as USERS via openIRIS system and receive the specific introduction by the CUSCO personnel.
Following account activation, you as a CUSCO user, agree to adhere to the following obligations:
- with the regulations of the user rules and the specific laboratory and equipment rules (see Service and Equipment).
- to attend the annual safety instructions for the necessary laboratory safety levels.
- to strictly follow the instructions of the CUSCO staff in the CUSCO rooms and when using the equipment.
- to make the CUSCO staff aware of the existence of safety risks related to the experimental material (e.g., pathogenic, infectious, toxic, or radioactive properties or according to Biostoffverordnung (BioStoffV)).
- if necessary, to provide evidence of corresponding notifications and approvals (here in particular the approval of the genetic engineering experiments to be carried out or experiments on animals or humans, if necessary, with the approval of the ethics committee) of research projects and experiments. Patient data are to be handled by the user in compliance with the relevant data protection regulations and are to be handed over to CUSCO exclusively in anonymized form.
- any work with genetically modified organisms (according to Gentechnikgesetz (GenTG)) is only allowed after a prior approval with the CUSCO director.
- if necessary, authorize CUSCO to perform the experiments.
- Operate the equipment and perform the experiments only after the instruction of CUSCO staff.
Service and equipment
Within the framework of its available capacities, the CUSCO performs the following services in particular:
- Project consultation and support
- Isolation of primary cells from patient material
- Reprogramming of cells into human induced pluripotent stem cells (hiPSCs)
- Banking, characterization, and quality control of hPSCs
- Genome editing using CRISPR/Cas
- Establishment of differentiation protocols
- Provision of cells differentiated from hiPSCs
- Establishment of methods for organoid generation and analysis
- Provision of hiPSC reference cell lines
- Provision of reagents
- Provision of infrastructure and equipment
- Training
In addition, CUSCO manages access to and supports, the shared equipment (specific equipment available is listed in openIRIS).
Equipment managed by CUSCO is available to all CUSCO registered users. By accepting these Terms of Service, the users agree to adhere to the following rules of the equipment use:
- Equipment access and training must be requested through openIRIS. Approval of access will be granted only to the users that completed the training.
- All equipment available at CUSCO must be booked through openIRIS before use. Use of equipment without booking is strictly forbidden.
- Equipment booking expires 30 min after the booking time has begun if the user is not present, but usage fees will apply.
- In case the use time exceeds the booking time, additional time must be booked in openIRIS.
- Bookings can be made up to a maximum of 14 days before planned use. Cancellation of a booking can be made up to 24 hours before the start of the booked period. Cancellations made after this time may result in usage fees if no other user books this period.
- Note that repeated absence during booked time, use of device without booking and/or misuse of equipment will result in user access cancellation.
- Users are responsible for immediate removal of data from the CUSCO computers. CUSCO is not responsible for data storage and safety. All data older than four weeks will be automatically deleted from the PCs without further notice.
- It is not allowed to store any kind of data anywhere else on the CUSCO computers other than in the respective directories. All data in other locations (for example on the desktop) will be deleted without further notice.
- Equipment manuals and accessories must remain with the respective device at any time (PDFs of manuals can be provided on request by CUSCO).
- In case of a device malfunction, the CUSCO personnel must be immediately informed. Error messages must be copied or documented by a screenshot and uploaded as an “issue” through openIRIS.
- Cells provided to the user must not be shared with other users or researchers outside of the core facility or without consent of the head of CUSCO.
Liability
All users are liable according to the legal regulations. This applies in particular to damage caused by failure to comply with the obligations incumbent on the users, by non-clarification of security risks or by non-compliance with binding instructions issued by the staff. The responsibility for the quality of the incoming material or incoming data of a project lies with the user.
The liability of CUSCO towards users is limited to intent and gross negligence. The CUSCO do not assume any warranty for the test material.
Genetically modifies organisms (GMO)
Regulations regarding working with and handling genetically modified organisms or materials containing genetically modified organisms are described in the Federal Republic of Germany by the Genetic Engineering Act (Gentechnikgesetz-GenTG).
All users requesting genetically modified material or genetic modification of iPSCs (Service: genetic modification) and/or bringing and working with genetically modified organisms in the CUSCO facility are required to inform the CUSCO personnel about such work and provide a copy of the official permission and registration of the genetic engineering facility / laboratory.
For Charité internal regulations see: https://intranet.charite.de/biosicherheit/startseite/
Publications
Users are obliged to take due account of the work of CUSCO in publications following the generally accepted rules of scientific practice:
- In principle, any external work, e.g., the work of CUSCO, must be identified at the appropriate points in scientific work, e.g., in the material and method part. The payment of fees or charges resulting from service does not replace the corresponding labelling of technical or scientific work.
- Depending on the scope and complexity of the work or in the case of corresponding collaboration agreements, the CUSCO or individual employees must be considered by mentioning them in the acknowledgment[1] or within the framework of a co-authorship. Co-authorship of CUSCO scientists usually requires a scientific contribution that goes far beyond the mere performance of experiments or analyses.
- The CUSCO should be notified before submission of the publication. If necessary, the corresponding files must be made available for inspection beforehand. Also, a copy of the publication in electronic form must be sent to the head of CF Stem Cells after publication.
Exclusion and restriction of use
In particular, the approval for use may be refused, revoked, or further restricted if
- no proper application has been submitted
- the information in the application is not or is no longer correct
- a fixed usage fee is not paid
- the regulations for use, the specific laboratory and equipment rules or instructions of CUSCO staff are violated
- CUSCO is not mentioned or adequately considered in publications according to chapter „Publications“.
The user is not entitled to claims for damages due to the refusal, revocation, or subsequent limitation of admission.
Service Catalogue / Usage Fees
- The fees to be paid by the users for CUSCO services can be found in the current list of fees. Special rates apply to external users.
- For projects regarding technology or method development, including feasibility studies, within the framework of a cooperation, the prices can be reduced or, in exceptional waived at CUSCO staff discretion. Co-authorships of CUSCO staff are expected to be considered in the resulting publications according to their scientific or technical contributions.
- The user fees are charged after receipt of the services. In the case of internal customers, this is done via internal service charging (interne Leistungsverrechnung, ILV). External users always receive an invoice.
- Project leaders can request user fees for funding applications (in DFG applications under the item „Requested other costs“, CUSCO is registered in the DFG Database). The team of CUSCO will be available to assist you with the appropriate cost estimation during the application phase.
- CUSCO reserves the right to issue a partial charge on the failed services. Due to underlying biological limitations, CUSCO is unable to always guarantee the success of the following services:
- Reprogramming
- Genome editing
- Differentiation
CUSCO staff guarantees to perform necessary experimental steps to fulfil the service request. However, in an unlikely event of the service failure, the number of further attempts will be limited as defined by individual service descriptions and the services will be continued only after an explicit confirmation from the User. The user agrees to the payment of charges according to the steps completed by CUSCO in the attempts to fulfil the service.
è The current list of fees is attached as Appendix 3.
Severability clause and validity of the user regulations
The user regulations are binding for all users of CUSCO. Agreements beyond the scope of these Terms of Service are made with external users and must be issued in writing. The Terms of Service are valid in the respective version without limitation until they are replaced by an updated version.
Contact
Director: Dr. Harald Stachelscheid
Charité – Universitätsmedizin Berlin
BIH Core Unit pluripotent Stem Cells & Organoids
Cranach Haus
Augustenburger Platz 1
13353 Berlin
(Visitors: Föhrer Straße 15)
Email: CUSCO@bih-charite.de
Appendix 1: Specific rules for equipment use
MACSQuant VYB:
- Users must use personal accounts. Account sharing is not allowed.
- Personal account will be assigned immediately after user introduction.
- Calibration of the machine is performed by the CUSCO personnel once a month.
MACSQuant Tyto FACS Sorter:
- Users must use personal accounts. Account sharing is not allowed.
- Workspace set-up and advanced training cannot be provided by CUSCO and must be requested by the user to Miltenyi directly. CUSCO only provides access to the Sorter, an account, and general recommendations for equipment use.
- Accessories, like Tyto magnet for cartridge load, must be used at CUSCO lab. It is strictly forbidden to remove equipment from CUSCO lab space.
- No gloves are permitted when working with the system.
- The computers operating this equipment must not be switched off. It is critical for the instrument operation that the computers stay active.
- Use of the computer for applications other than device control is not allowed.
- The use of USB storage devices is prohibited.
- must be trained in the operation of the unit by an experienced user. After this training, CUSCO staff will evaluate the ability of the user to operate the unit properly and permit access.
- Measurement data must be transferred to the Columbus server (http://columbus.charite.de) or OMERO Server (https://omero.charite.de) immediately after the measurement and removed from the measurement computer.
Leica inverted Microscope (User Lab):
- Data must be saved in a folder named after the group/username in the following location:
“Dieser PC > Dokumente > Folder [Group Name] > [ username]”
- Data must be saved in a folder named after the group/username in the following location:
“D:> cap_0.5.1.4182005e > folder [username]”QuantStudio 6:
- Data must be saved in a folder named after the group/username in the following location: “D:UsersPublicDocumentsQuantStudio Real-Time PCR softwareexperiments[Group Name][user name]”
Opera Phenix, Operetta and Columbus Server
- All image data should be moved immediately after acquisition to the central OMERO Server (https://omero.charite.de) of the Charité
Picking hood Microscope (User Lab):
- Data must be directly saved in external source (USB stick) and/or deleted immediately from the computer hard disk after transfer.
Chromium Controller
- Only the use of the Controller and accessories ad-hoc are allowed (chip holder and vortex with adaptor for beads rack).
- Accessories must be used at CUSCO lab; it is not allowed to move them somewhere else.
Molecular biology work bench rules
- Molecular biology bench must be booked via openIRIS when Chromium Controller is used.
- This bench is reserved for single cell experiments. Cryostat
- It is mandatory to clean up the device after use with ethanol 80% as it was instructed during the training. Remnants of embedding medium can damage the device.
Leica LSM SP8
- Use of the computer for applications other than device control is not permitted.
- The use of USB storage devices is prohibited.
- All image data should be moved immediately after acquisition to the central OMERO Server (https://omero.charite.de) of the Charité
INCUCYTE – User rules
- It is mandatory to receive training from CUSCO. Training can be booked using OpenIris.
- Only the use of a personal account is allowed.
- Incucyte software can be downloaded in every Charité PC (instructions will be provided during the training). If needed the Working station (ground floor, room 0.0134) can be used to schedule experiments. It is not permitted to use other CUSCO computers.
- Incucyte use is allowed only during booked time slots. Booking has to be scheduled using OpenIris.
- Booking is mandatory for the whole time of experiment/the plates are in the Incucyte.
- ONly 2 concurrent bookings are possible. Each booking allows the use of 2 (out of 6) Incucyte plate-positions (i.e. 1 user = 1 booking slot = 2 positions of the tray).
- 2 (out of 6) spots of the tray are reserved for single scannings (“Scan now” acquisition type). Therefore, those 2 spots HAVE to be free and only used during the single scan (limited time - single time).
- Each booking (up to 2 plates) can use a schedule of a maximum of 30 minutes duration per hour.
- It is mandatory to leave every 3 hours 1 slot of 30 minutes free for spontaneous measurements
- Do book your slots time ahead (preferably 1 week) to avoid undesirable overlapping between users. Booking can be cancelled until 1 hour before it starts (please contact CUSCO member if needed). Booking cancel within 1 hour prior starting will be charged.
- Plate loading has to be done carefully and holding the tray from below.
- You HAVE to remove your plate after the experiment has finished and delete the acquisition schedule from the Software to allow next measurement. If plate is abandoned (no openIRIS booking visible), CUSCO will discard it without further notice.
- If you notice before starting your experiment that water in the incubator is missing or any other anomaly (not signaled by alarm) please contact a CUSCO member.
- Data will be deleted every 2 months. During this time you can analyze and archive your data after downloading.
- Data export to the ImageArtist server is possible. Please contact CUSCO member to learn how to proceed.
- Do not set for acquisition empty wells, focus will not be found and the whole scanning may stop.
Appendix 2: Specific rules for use Cell culture labs
- It is mandatory to book cell culture safety cabinet in openIRIS before use. In case the use time exceeds the booking time, additional time must be booked in openIRIS.
- Use of PPE (lab coat and gloves) is mandatory in the cell culture labs.
- User responsibilities:
è Refill general consumables, for example: 80% ethanol, paper tissues, glass pipettes, plastic material, etc.
è Dispose of trash as indicated during lab introduction (white bins, yellow glass containers, already full trash bags).
è Empty liquid waste (vacuum container).
è Inform CUSCO personnel if reagents or consumables are running out (use the white board outside of the cell culture room to leave notices). Provide enough time before the material is completely consumed.
è Inform CUSCO personnel if any issue with equipment or space is encountered.
è Keep safety cabinets, incubators, water bath and general space clean.
è Re-fill water in incubator if needed using deionized autoclaved water (red cap bottles).
è Only pipette tips with filter are allowed in the cell culture safety cabinets. Please store only one box of pipette tips per size for each bench and make sure they are closed properly when removed from the safety cabinet.
- It is not allowed to stock a large number of aliquots of reagents from the user projects in the fridge/freezer of the user lab. Keep only what is needed in the space assign to you for a month. Space is limited.
- All reagents, plates, boxes, etc. must be properly and clearly labelled. The label must include: the Group, Initials and Date. CUSCO personnel will discard all unidentifiable material without further notice.
- It is not allowed to store samples (DNA, RNA, cell pellets, imaging plates, etc.) in fridge/freezer of the user lab for long term. When a user is not working in the facility anymore (not active) and after notification, we will remove and discard all reagents/samples of the user. Do not store any belongings on the benches/cupboards, they will be removed. It is a common working area.
- After completion of work:
- Clean bench and disinfect the surface with 80% ethanol!
- Clean the tube of the pump first with deionized water and subsequently with 80% ethanol after usage
- Switch off lamps of the microscopes according to the introduction!
- If necessary (last user in the cell culture lab), switch off the bench, pump, water bath and microscope.
- The following materials will be provided by CUSCO and are included in the bench usage fee:
- Serological Pipettes (5ml, 10ml and 25ml)
- Falcon Tubes (15ml and 50ml)
- Plates (6-wells and 12-wells)
- Filter Tips
- Ethanol
- Yellow waste bins
- Gloves
- EDTA solution
- Eppendorf tubes
Furthermore, the following reagents are provided but must be purchased in openIRIS prior use:
- Vitronectin
- ROCK inhibitor
- mTeSR medium
- E8 medium
Appendix 3:Price list of CUSCO internal users (billing via ILV)
| Description | Unit | Price [€] |
|---|---|---|
| Generation of iPSCs, Banking and Characterisation | ||
| Primary cell Isolation | Donor | 90,00 |
| Derivation of iPSCs from primary cells | Donor | 3550,00 |
| Banking of iPS cell line (48 vials) | cell line | 640,00 |
| QC and Characterisation - basic Panel | cell line | 1230,00 |
| Cell Line Identity (STR Analysis) | cell line | 150,00 |
| Virtual Karyotyping using a SNP Array | cell line | 280,00 |
| G-Banding Karyotyping | cell line | 450,00 |
| Morphology and Viability | cell line | 290,00 |
| Sterility and Mycoplasma testing | cell line | 70,00 |
| Marker for undifferentiated iPSCs (IF) | cell line | 340,00 |
| Marker for undifferentiated iPSCs (FACS) | cell line | 400,00 |
| Differentiation capacity of hPSCs | cell line | 640,00 |
| Confirmation of gene editing | cell line | 80,00 |
| Virologic testing of cultured cells | cell line | 510,00 |
| Bank of single cell culture adapted hPSC | cell line | 1710,00 |
| Differentiated Cells and Organoids | ||
| Generation of endothelial cells | BATCH | 1250,00 |
| Generation of hepatocytes | PLATE | 720,00 |
| Generation of neural stem cells | BATCH | 1390,00 |
| Generation of iNeurons (NGN2 induced) | BATCH | 480,00 |
| Celebral organoids - StemCellTech Kit | BATCH | 1460,00 |
| Generation of human Lung Organoids (hLOA) | BATCH | 980,00 |
| Passaging and expansion of hLOA | BATCH | 200,00 |
| Generation of human Intestinal Organoids (hIO) | BATCH | 580,00 |
| Passaging and expansion of hIO | BATCH | 420,00 |
| Gene editing and complex projects | ||
| Supervision | hour | 41,00 |
| CRISPR Design | cell line | 210,00 |
| gRNA Efficiency testing | cell line | 650,00 |
| CRISPR experiment | cell line | 530,00 |
| Single cell cloning with IOTA | cell line | 1000,00 |
| Clone Validation | cell line | 1380,00 |
| Provision of reagents | ||
| E8 Media (500ml) - own production | BOTTLE | 84,00 |
| E8 Media (500ml) - own production KIT | BOTTLE | 84,00 |
| mTeSR Media (500ml) - own production | BOTTLE | 121,00 |
| mTeSR Media (500ml) | BOTTLE | 395,00 |
| Geltrex Aliquots (10 ml) | Aliquot | 82,00 |
| Vitronectin Aliquots (600 µl) | Aliquot | 81,00 |
| ROCK Inhibitor Y-27632 (1 ml) | Aliquot | 68,00 |
| Provision of cells | ||
| Provision of live iPSCs | WELL | 21,00 |
| Cryopreserved iPCSs | VIAL | 79,00 |
| Cryopreserved iPCSs - own bank | VIAL | 15,00 |
| Cryopreserved Neuronal Stem Cells | VIAL | 30,00 |
| Cryopreserved Endothelial Cells | VIAL | 44,00 |
| Devices | ||
| FACS Analyzer MACS Quant VYB | hour | 13,00 |
| FACS Sorter MACS Quant Tyto | hour | 26,00 |
| Real-time PCR Quantstudio6 | hour | 8,00 |
| 10X Chromium Controller | hour | 63,00 |
| Leica Confocal SP8 | hour | 13,00 |
| Opera Phenix High Content Screener | hour | 16,00 |
| Opera Phenix High Content Screener-off h | hour | 5,00 |
| IsoCell | hour | 27,00 |
| TapeStation | hour | 8,00 |
| Incucyte SX5 | hour | 3,00 |
| Cell3Imager Duos | hour | 13,00 |
| Bench fee - User-Lab | hour | 10,00 |
| Training | ||
| Equipment training | hour | 47,00 |
| Hands-on training - Basic hPSC culture | training | 620,00 |
| Hands-on training - iNeurons | training | 530,00 |
| Hands-on training - NSCOs | training | 320,00 |
| Hands-on training - hLOA generation | training | 380,00 |
| Hands-on training - hIO generation | training | 500,00 |
| Hands-on training - Endothelial cells | training | 780,00 |
[1] Please refer to the following formulation example: „This work was supported by the Core Unit pluripotent Stem Cells and Organoids of the Berlin Institute of Health (BIH) at Charité – Universitätsmedizin Berlin.”
Provider Location: Charité Campus Virchow-Klinikum (CVK), Berlin
Name: EEG-NIRS-System
Resource type: Equipment
Provider: Shared Equipment at Charité
Affiliations: Charité
Description: This EEG-NIRS system provides the ideal equipment for neuroimaging in developmental populations. Both EEG and NIRS components have been previously approved in developmental research and can be used in their combined or single application.
Electroencephalograpy (EEG):
actiCHampPlus 32 (8+) system with active electrodes (Brain Products GmbH)
Near-infrared spectroscopy (NIRS):
NIRScout Model 812 & NIRScout S Core System Unit (NIRx Medical Technologies LLC)
Contacts: Prof. Dr. Claudia Männel (claudia.maennel@charite.de, sprachentwicklung@charite.de)
Comments: If you would like to use the EEG and/or NIRS equipment in our development laboratory, please contact us via email (see contacts).
Provider Location: Berlin
Name: EnviroESCA
Resource type: Spectroscopy
Provider: Surface analytics unit - BCP
Affiliations: FU Berlin
Description:
Surface elemental analysis at near ambient/UHV conditions.
Booking of the EnviroESCA is restricted to trained key users of the working groups. If you are destined to become a key user for this device for your working group, please contact the resource admin(s).
Contacts: Dr. Philip Nickl (p.nickl@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to key users and are possible at working days. The minimum booking time is 3 hours.
!Bookings are possible only for trained users!
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB, Altensteinstraße 23A, 14195 Berlin, Germany
Name: IONTOF M6
Resource type: Mass Spectrometer
Provider: Surface analytics unit - BCP
Affiliations: FU Berlin
Description: Surface analysis at near ambient/UHV conditions.
Booking of the IONTOF M6 is restricted to trained key users of the working groups. If you are destined to become a key user for this device for your working group, please contact the resource admin(s). Training can be also requested from the timeline view in the scheduler (top right, booklet symbol).
Contacts: Dr. Philip Nickl (p.nickl@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained key users and are possible at working days. Minimum booking time 3 hours.
Sample preparation should be discussed with the measuring person in advance.
!Bookings are possible only for trained users!
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB, Altensteinstraße 23A, 14195 Berlin, Germany
Name: TGA 8000
Resource type: Gravimetric analysis
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Thermal gravimetric analysis (heating of the sample rt-1200 °C.)
Contacts: Dr. Christian Halbig (christian.halbig@fu-berlin.de), Jasmin Er (jasmin.er@fu-berlin.de), Taylor Page (taylor.page@fu-berlin.de), Sofiia Zuieva (sofiia.zuieva@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
OFFLINE - FURNACE BROKEN
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: IR Spectrum Two
Resource type: Spectrometer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description:
Fourier-transform Infrared Spectroscopy (IR spectrum from 4000-650 cm-1.)
- ATR stage available
- Cuvette holder available
- Demountable cell available
Contacts: Taylor Page (taylor.page@fu-berlin.de), Christian Zoister (christian.zoister@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days. Minimum booking time 1 hour.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: UV-Vis Lambda 365
Resource type: Spectrometer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: UV-Vis Spectroscopy (UV spectrum from 190-1100 nm)
Contacts: Alexander Krappe (a.krappe@fu-berlin.de), Sebastian Müller (sebamue@zedat.fu-berlin.de), Taylor Page (taylor.page@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days. Minimum booking time 1 hour.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: MicroCal PEAQ-ITC
Resource type: Calorimeter
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Isothermal titration calorimeter
Contacts: Weronika Malicka (malickaw@zedat.fu-berlin.de, Prof. Dr. Matthias Ballauff (mballauff@zedat.fu-berlin.de)
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: MicroCal VP-ITC
Resource type: Calorimeter
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: isothermal titration calorimeter
Contacts: Weronika Malicka (malickaw@zedat.fu-berlin.de), Prof. Dr. Matthias Ballauff (mballauff@zedat.fu-berlin.de)
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: MPT-2 Mehrzweck Titrator
Resource type: Particle size analyzer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Titrator for atomated DLS measurements
Contacts: Daniel Braatz (danielbraatz@zedat.fu-berlin.de), Justin Arenhoevel (justin.arenhoevel@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: Zetasizer Ultra "new"
Resource type: Particle size analyzer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Multi-Angle dynamic light Scattering (MADLS), Zeta potential
Contacts: Taylor Page (taylor.page@fu-berlin.de), Fernanda Romero (fernanda.romero@fu-berlin.de), Justin Arenhoevel (justin.arenhoevel@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: Zetasizer NanoZS "red"
Resource type: Particle size analyzer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: dynamic light scattering with possibility to do zeta-potential measurements
Contacts: Taylor Page (taylor.page@fu-berlin.de), Fernanda Romero (fernanda.romero@fu-berlin.de), Justin Arenhoevel (justin.arenhoevel@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: Fluorescence FL 6500
Resource type: Spectrometer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Fluorescence Spectroscopy (excitation and emission wavelength range up to 900 nm)
Contacts: Alexander Krappe (alexander.krappe@fu-berlin.de), Jan Soyka (j.soyka@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days. Minimum booking time 1 hour.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Calibration performed 18th March 2024, excitation was off by ~10 nm.
If you have problems with the cooling unit, please contact Dr. Philip Nickl or Jan Soyka. The cooling unit is slightly louder than normal but working.
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: QCM-D (Quartz Crystal Microbalance)
Resource type: Other Instruments
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: A quartz crystal microbalance with dissipation monitoring (QCM-D) is a type of quartz crystal microbalance (QCM) based on the ring-down technique. It is used in interfacial acoustic sensing. Its most common application is the determination of a film thickness in a liquid environment (e.g., adsorbed protein layer, SAMs). It can be used to investigate further properties of the sample, most notably the layer's softness.
Contacts: Andrea Cosimi, (andreac92@zedat.fu-berlin.de), Peng Tang (mauricetangp@zedat.fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: Proteomic MS Analysis of Biomaterials
Resource type: Mass Spectrometer
Provider: Imaging Mass Spectrometry Unit
Affiliations: Charité, BIH
Description: Proteomic characterization of therapeutic biomaterials (ECM, scaffolds and patches), tissue biopsies by using high standardized and robust LC-Based Mass Spectrometry.
Contacts: oliver.klein@bih-charite.de
Provider Contact: oliver.klein@charite.de
Provider Location: Charité - Universitätsmedizin Berlin Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin
Name: Proteomics MS-Analysis of therapeutic cell products
Resource type: Mass Spectrometer
Provider: Imaging Mass Spectrometry Unit
Affiliations: Charité, BIH
Description: Proteomic characterization of therapeutic cell products (e.g., Immune cells) by using high standardized and robust LC-Based Mass Spectrometry.
Contacts: oliver.klein@bih-charite.de
Provider Contact: oliver.klein@charite.de
Provider Location: Charité - Universitätsmedizin Berlin Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin
Name: Imaging Mass Spectrometry of FFPE Tissue section
Resource type: Imaging
Provider: Imaging Mass Spectrometry Unit
Affiliations: Charité, BIH
Description: In situ tissue assessment of spatial molecular signatures by MALDI-Mass Spectrometry Imaging (MALDI-MSI) to aid in the development of classifiers for therapy stratification and diagnosis/prognosis. This workflow includes: 1) tissue slicing, 2) tissue pre-preparation 3), MALDI-MSI analyses and chemical staining (e.g., HE for data co-registration).
Contacts: oliver.klein@bih-chartie.de
Provider Contact: oliver.klein@charite.de
Provider Location: Charité - Universitätsmedizin Berlin Campus Virchow-Klinikum, Augustenburger Platz 1, 13353 Berlin
Name: Seminar Room 001
Resource type: Room
Provider: Fachbereich Veterinärmedizin
Affiliations: FU Berlin
Description: Large Seminar Room
Contacts:
Comments:
Recurring bookings are possible; please remember to define the end date!
! Request based booking !
Provider Location: Berlin
Name: Seminar Room 114
Resource type: Room
Provider: Fachbereich Veterinärmedizin
Affiliations: FU Berlin
Description: Small Seminar Room
Contacts:
Comments: 15 seats (more may be possible), equipped with a smartboard.
Recurring bookings are possible; please remember to define the end date!
! Request based booking !
Provider Location: Berlin
Name: Idea office appointment
Resource type: Meeting Room
Provider: Idea Office
Affiliations: Charité, BIH
Description: Booking a meeting to discuss:
- Contacts to Charité-internal Idea-Office
- Information on "patents"
- Information on intellectual property rights – queries on contract administration and management
- Information on subsidiary programs and funds
- Assistance in filing an application
- Contact to companies
- Information on "spin-off creation"
- Information on "regulatory affairs"
Contacts: thomas.wallach@charite.de, bettina.otto@charite.de
Comments: Please select a 15 Minute slot. If the timeslot does not work for you please contact us (bettina.otto@charite.de and thomas.wallach@charite.de)
Provider Location: Charité BIH Innovation - The technolgy transfer of BIH and Charité
Name: Register my group with FB Veterinärmedizin
Resource type: Administration
Provider: Fachbereich Veterinärmedizin
Affiliations: FU Berlin
Description:
If your group is not yet installed in OpenIRIS, it will be created
concurrently, if your group is from FUB. External groups, however, must
be created by their home institution before access to FB Veterinärmedizin can be
provided.
In case of a representative puting the request, the group head must sign the form, which thereafter must be sent to Benjamin-F. Hempel (benjamin.hempel@fu-berlin.de) or Andreas Nerlich (andreas.nerlich@fu-berlin.de). Please do also appoint a second group admin that can take over administrative tasks for the group in OpenIRIS.
Contacts:
Comments: Group access to FB Veterinärmedizin is necessary to allow all group members to book resources of the core facility.
Preferably, the request should be put by the group head.
Provider Location: Berlin
Name: Leica Stellaris 8
Resource type: Microscope
Provider: Shared Equipment at Charité
Affiliations: Charité
Description:
Imaging modes
- Scanning confocal (including XZ direction scans)
- Wide field (DAPI, FITC, YFP, RHOD, TXR)
- Video-imaging up to 60 fps
- TauSense, TauSeparation, TauGating: functional imaging based on arrival times
- FRAP
- Live-cell incubator (temperature, CO2, humidity)
- Spectral scans
- Lightning mode (near super-resolution)
Lasers and max. intensities out of 60x oil objective
- 405 nm
- White light laser: 440-700 nm (pulsed)
Emission Filters
| Filter | λ/Bandwidth |
| DAPI | 460/50 |
| FITC | 527/30 |
| YFP | 535/30 |
| RHOD | 585/40 |
| TXR | 630/75 |
Scanner
- AOBS scanner
Detectors
- 2x Power Hybrid Detektor HyD S
- 2x Power Hybrid Detektor HyD X
- Transmission light detector (DIC, phase)
- Spectral detection
Objectives
| Mag. | Type | Immer. | NA | WD [μm] | Info |
| 10x | HC PL FLUOTAR | Air | 0.3 | 11,000 | |
| 20x | HC PL FLUOTAR | Air | 0.55 | ||
| 20x | HC PL APO | Water (multi imm) | 0.75 | 670 | |
| 40x | HC PL APO | Oil | 1.30 | 240 | |
| 63x | HC PL APO | Water | 1.20 | 300 | motCorr |
| 2.5x | HC PL FLUOTAR | Air | 0.07 | 9,200 |
Special
- Super Z-galvo stage
- High precision LMT scanning stage
- Automatic water immersion for long-term imaging
- Resonant scanner for fast imaging
Contacts: Dr. Anita Balázs (anita.balazs@charite.de)
Comments: To get access to the microscope please click on the request access icon.
Provider Location: Berlin
Name: 20. AMBIO STED Abberior Facility Line
Resource type: Microscope
Provider: AMBIO
Affiliations: Charité
Description:
Contacts: joerg.piontek@charite.de; dorothee.guenzel@charite.de
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany
Name: Sigma 6-16KS
Resource type: Centrifuge
Provider: Eigler Lab
Affiliations: FU Berlin
Description:
Refrigerated benchtop centrifuge.
THE USE OF THE CENTRIFUG HAS TO BE NOTED IN THE PHYSICAL LOG BOOK
Contacts: Christian Halbig (christian.halbig@fu-berlin.de)
Provider Contact: christian.halbig@fu-berlin.de
Provider Location: Altensteinstraße 23A 14195 Berlin, Tel. 838 63201
Name: SONOPULS HD-3100
Resource type: Sonicator
Provider: Eigler Lab
Affiliations: FU Berlin
Description: Ultrasonic homogeniser
Contacts: Christian Halbig (christian.halbig@fu-berlin.de)
Provider Contact: christian.halbig@fu-berlin.de
Provider Location: Altensteinstraße 23A 14195 Berlin, Tel. 838 63201
Name: Thermo Q Exactive HF (LC-MS)
Resource type: Mass Spectrometer
Provider: Bio Mass Spectrometry unit - BCP
Affiliations: FU Berlin
Description: hybrid quadrupole-Orbitrap mass spectrometer
Contacts: Dr. Benno Kuropka (kuropka@zedat.fu-berlin.de)
Provider Contact: kuropka@zedat.fu-berlin.de
Provider Location: Thielallee 63, 14195 Berlin
Name: bioMS measurements (bioMS - BSM)
Resource type: Mass Spectrometry
Provider: Bio Mass Spectrometry unit - BCP
Affiliations: FU Berlin
Description: Every request demands a brief description of the sample and the preparation protocol, and must be approved by the group head of the requester. Only after this confirmation, the service can be initiated. We will then, together with the requester, decide on the
necessary steps prior to the data acquisition.
Contacts: Dr. Benno Kuropka (kuropka@zedat.fu-berlin.de)
Comments:
Request of a bioMS measurement project is open to all users.
Provider Contact: kuropka@zedat.fu-berlin.de
Provider Location: Thielallee 63, 14195 Berlin
Name: Bruker D8 Venture
Resource type: X-ray Diffractometer
Provider: X-Ray diffraction unit - BCP
Affiliations: FU Berlin
Description: This Bruker D8 Venture single crystal diffractometer is available for non-assisted measurements for trained persons from FUB. It is equipped with two x-ray sources (Cu and Mo), a Photon II detector, and an oxford cryostream 700.
Contacts: Dr. Anja Wiesner (anja.wiesner@fu-berlin.de)
Comments: Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible in three predetermined blocks.
Provider Contact: anja.wiesner@fu-berlin.de
Provider Location: Fabeckstraße 34-36, 14195 Berlin
Name: OMERO Collab Group Request
Resource type: Request
Provider: OMERO Microscopy Image Data Management
Affiliations: Charité, BIH
Description: Request to create a collaboration group in OMERO.
Contacts: Omero support
Comments:
Request to create a collaboration group in OMERO.
Provider Location: Charité Universitätsmedizin Berlin
Name: Circular Dichroism Spectrometer (Olis DSM 20)
Resource type: Spectrometer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Olis DSM 20 Circular Dichroism Spectrometer
Contacts: PhD Kenichi Ataka (ataka@zedat.fu-berlin.de), Eunjin Moon (eunjin.moon@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days. Minimum booking time 1 hour.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB (FU-Berlin), Altensteinstraße 23a, 14195 Berlin, room 220
Name: Kibron MicroTrough XS
Resource type: Coater
Provider: Eigler Lab
Affiliations: FU Berlin
Description: Langmuir-Blodgett trough
Contacts: Christian Halbig (christian.halbig@fu-berlin.de)
Provider Contact: christian.halbig@fu-berlin.de
Provider Location: Altensteinstraße 23A 14195 Berlin, Tel. 838 63201
Name: Glovebox Surface Analytics Lab 028
Resource type: Lab Space
Provider: Surface analytics unit - BCP
Affiliations: FU Berlin
Description:
Preparation, handling and storage of air- or moisture-sensitive samples under nitrogen atmosphere.
Booking of the glovebox is restricted to trained key users. If you are destined to become a key user for this device for your working group, please contact the resource admin(s).
Contacts: M.Sc. Jasmin Er (jasmin.er@fu-berlin.de), Dr. Philip Nickl (p.nickl@fu-berlin.de)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to key users and are possible at working days.
!Bookings are possible only for trained users!
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB, Altensteinstraße 23A, 14195 Berlin, Germany
Name: Tube Furnace Nabertherm
Resource type: Heating
Provider: Eigler Lab
Affiliations: FU Berlin
Description: Nabertherm with B410 controller
Contacts: christian.halbig@fu-berlin.de, qing.cao@fu-berlin.de
Comments: Temperature 1200 °C
Provider Contact: christian.halbig@fu-berlin.de
Provider Location: Altensteinstraße 23A 14195 Berlin, Tel. 838 63201
Name: Axio Imager Vario
Resource type: Microscope
Provider: Core Facility for Electron Microscopy
Affiliations: Charité
Description: Suitable for LM questions from semithin section to IFM.
Contacts: cfem@charite.de
Affiliated Communities: ACEM
Provider UsagePolicy: https://elektronenmikroskopie.charite.de/fileadmin/user_upload/microsites/ohne_AZ/m_cc02/elektronenmikroskopie/Nutzerordnung_01072020.pdf
Provider Contact: cfem@charite.de
Provider Location: Campus Charité Mitte (CCM) and Charité Campus Virchow-Klinikum (CVK), Berlin
Name: MicroCT RX-Solutions
Resource type: Other Instruments
Provider: Dentistry Research Groups
Affiliations: Charité
Description: With the EasyTom S, one simple scan allows you to see through your parts: internal/external structures, defects, porosities... visualize, measure, and analyze whatever the sample.
Contacts: paul.zaslansky@charite.de
Provider Location: Berlin, Germany
Name: Aurora "Paris" - Full Spectrum Analyzer
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility (BIH CCF)
Affiliations: Charité, BIH
Description: 5-Laser full spectrum analyzer
Contacts: jacqueline.keye@bih-charite.de
Comments:
You need to be registered and trained to use this instrument.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Provider UsagePolicy:
The user guidelines have been updated. Updated user guidelines and current services and prices can be found on our website.
With this you confirm that you are aware of and accept the updated version of the user guidelines and price list.
Provider Contact: desiree.kunkel@bih-charite.de
Provider Location: Charité Campus Virchow-Klinikum (CVK) & Campus Charité Mitte (CCM), Berlin
Name: Device 3 Access Form
Resource type: Form
Provider: Test Provider
Affiliations: Charité, BIH
Description: Access form to get Device 3 access
Contacts: openiris@charite.de
Comments: Please submit form to get access to Device 3
Provider Contact: openiris@charite.de
Provider Location: Charite
Name: Service measurement XPS (SA unit - BSM)
Resource type: Spectrometer
Provider: Surface analytics unit - BCP
Affiliations: FU Berlin
Description: abc
Contacts: Dr. Philip Nickl (mail: p.nickl@fu-berlin.de; phone: 58037)
Comments: You apply for assisted surface characterization (service measurement) at the Surface Analytics Unit. The surface characterization of compounds/materials is charged. By this request you accept the obligation of coverage of the costs for the measurements. User fees are listed here: www.biosupramol.de. You will be contacted as soon as possible to discuss further proceeding.
Provider Contact: p.nickl@fu-berlin.de
Provider Location: SupraFAB, Altensteinstraße 23A, 14195 Berlin, Germany
Name: STOE IPDS 2T
Resource type: X-ray Diffractometer
Provider: X-Ray diffraction unit - BCP
Affiliations: FU Berlin
Description: This Oxford Diffraction Xcalibur Nova O single crystal diffractometer is available for non-assisted measurements for trained persons from FUB. It is equipped a Cu x-ray sources, an Onyx CCD detector, and an Oxford CryojetXL.
Contacts: Dr. Anja Wiesner (anja.wiesner@fu-berlin.de)
Comments: Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible in three predetermined blocks.
Provider Contact: anja.wiesner@fu-berlin.de
Provider Location: Fabeckstraße 34-36, 14195 Berlin
Name: 12. AMBIO Zeiss Upright LSM 900 Airyscan 2
Resource type: Confocal Microscope
Provider: AMBIO
Affiliations: Charité
Description:
AMBIO Zeiss Upright LSM 900 Airyscan 2
Compact confocal for gentle multiplex (multi-channel, XYZT-dimensions, automation) imaging:
- 4 laser lines
- 2x GaAsP detectors, 1x Airyscan2 detector, 1x transmitted light detector
- Upright stage suited for fixed slides
- Monochromatic fluorescence camera
- Experiment-designer for optimal channel/ filter settings & acquisition speed
- Product page: https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html
Imaging modes
- Super-resolution & higher speed with Airyscan 2 (32x-array-detector for pixel re-assignment)
- Joint deconvolution of Airyscan 2 images for higher resolution
- Scanning confocal (DAPI, GFP, mCherry, Cy5, transmitted light)
- Improved confocal image quality with LSM Plus (linear Wiener filter for enhanced signal to noise & improved resolution)
- FRAP/PA (photo activation/conversion)
- Multiplex SR-2Y (increased speed with parallel scanning of 2 lines)
Lasers
- 385 nm
- 475 nm
- 555 nm
- 630 nm
Objectives
- 2.5x Plan-Apochromat (air), NA=0.085, working distance (WD)=8800 µm
- 10x Plan-Apochromat (air), NA=0.45, WD=2100 µm
- 20x Plan-Apochromat (air), NA=0.8, WD=550 µm
- 25x LD LCI Plan-Apochromat (multi immersion: water, silicone, glycerol, oil), NA=0.8, WD=570 µm, DIC
- 40x LD LCI Plan-Apochromat (multi immersion: water, silicone, glycerol), NA=1.2, WD=410 µm, --> ONLY this objective is optimized for Airyscan mode!
- 63x Plan-Apochromat (oil), NA=1.4, WD=190 µm, DIC
Contacts: ambio@charite.de
Comments: Access upon request of analysis project here.
Provider UsagePolicy: https://ambio.charite.de/fileadmin/user_upload/microsites/ohne_AZ/sonstige/ambio/Procedures/AMBIO_User_Guideline_and_Rules_2017-12-18.pdf
Provider Contact: ambio@charite.de
Provider Location: Campus Charité Mitte (CCM), CharitéCrossOver (CCO), Rm 01-306, Virchowweg 6, 10117 Berlin, Germany