OpenIRIS Resources
Name: CantoII "Thalia"
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description: Instrument configuration (download)
Comments:
You need to be registered and trained to use this instrument.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Name: CyTOF2/Helios "Tangerine"
Resource type: Mass Cytometer
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description: Flow Cytometry and Mass Spectrometry fell in love and a new technology was born: Mass Cytometry, the best of both worlds.
As in conventional flow cytometry, cells within a
heterogeneous sample are analysed on the single cell level for a
multitude of parameters. However, instead of using fluorochrome-labelled
antibodies to bind to the antigen of interest and being limited to the
number of spectrally resolvable fluorochromes, mass cytometry uses metal
isotope-labelled antibodies that are analysed by their time-of-flight
(TOF). This allows for the analysis of currently >40 parameters per
cell and a deep profiling of phenotype and function of cells.
Comments:
Usage of mass cytometry can be requested in "services".
- Please inform us about any changes in the acqusition template before you bring the samples.
Please contact the core facility at least 6 weeks before starting new projects to discuss technical details and instrument availability.
Name: Mass Cytometry (CyTOF) - Sample Acquisition
Resource type: Mass Cytometry
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description: Flow Cytometry and Mass Spectrometry fell in love and a new technology was born: Mass Cytometry, the best of both worlds.
As in conventional flow cytometry, cells within a heterogeneous sample are analysed on the single cell level for a multitude of parameters. However, instead of using fluorochrome-labelled antibodies to bind to the antigen of interest and being limited to the number of spectrally resolvable fluorochromes, mass cytometry uses metal isotope-labelled antibodies that are analysed by their time-of-flight (TOF). This allows for the analysis of currently >40 parameters per cell and a deep profiling of phenotype and function of cells.
Comments: Please request a project meeting at least 6 weeks before start of a new project to get access.
You can check CyTOF2/Helios availability before submitting a request, but be aware that not every available slot will fit for your specific measurement.
Operating hours: tue-thu 10:00-20:30, fr 10:00-17:30
Sample Acquisition Template: download
Name: MRT1 - Siemens Prisma 3T
Resource type: Magnetic Resonance Imaging System
Provider: BCAN (Berlin Center for Advanced Neuroimaging)
Affiliations: Charite
Description: The Magnetom Trio is Powered by the Tim Technology that allows you to achieve the highest level of image quality for your patients. With this MRI system, you are able to streamline your planning and processing and be able to diagnose your patient’s most challenging diseases very quickly with confidence. This system delivers high gradient performance that is needed for the most demanding MRI applications.
Important documents:
- measurement protocol / Messprotokol
- visitor screening form / Fragebogen für Besucher
- information sheet for healthy subjects / Informationsblatt für gesunde Probanden
- form regarding the handing over of subject data at BCAN / Formular zur Aushändigung von Probandendaten am BCAN
Comments: Access to the MRI machine is only granted upon registration of a study here (after login) or here. If the study you are working for is already registered with us, you can ask the responsible person to be added as a participant.
Bookings have to be approved by the facility (indicated by '-'). You will receive an email once your booking is approved.
Name: MRT2 - Siemens Prisma 3T
Resource type: Magnetic Resonance Imaging System
Provider: BCAN (Berlin Center for Advanced Neuroimaging)
Affiliations: Charite
Description: The Magnetom Trio is Powered by the Tim Technology that allows you to
achieve the highest level of image quality for your patients. With this
MRI system, you are able to streamline your planning and processing and
be able to diagnose your patient’s most challenging diseases very
quickly with confidence. This system delivers high gradient performance
that is needed for the most demanding MRI applications.
Important documents:
- measurement protocol / Messprotokol
- visitor screening form / Fragebogen für Besucher
- information sheet for healthy subjects / Informationsblatt für gesunde Probanden
- form regarding the handing over of subject data at BCAN / Formular zur Aushändigung von Probandendaten am BCAN
Comments: Access to the MRI machine is only granted upon registration of a study here (after login) or here. If the study you are working for is already registered with us, you can ask the responsible person to be added as a participant.
Bookings have to be approved by the facility (indicated by '-'). You will receive an email once your booking is approved.
Name: Paraffin embedding center
Resource type: Embedding Station
Provider: iPATH.Berlin
Affiliations: Charite
Description:
All modules of the embedding station operate at the same working height and are arranged according to lab requirements. The system with separate temperature control elements consists of a dispensing element, a preheating unit and a separate cooling plate. We provide moulds and forceps.
Comments:
Please contact us via email (see contacts) if you are interested in using the instrument. You will receive a short introduction prior to getting access to the instrument.
Booking slot duration: 30 - 90 min
Free cancellation of bookings at least 24 hours before start.
Name: Tecnai G2 20
Resource type: Electron Microscope
Provider: Core Facility for Electron Microscopy
Affiliations: Charite
Description: Suitable for high resolution EM, cryo-EM and tomography.
Name: Leo TEM 906
Resource type: Electron Microscope
Provider: Core Facility for Electron Microscopy
Affiliations: Charite
Description: Suitable for standard ultrastructural analysis.
Name: Gemini SEM 300
Resource type: Electron Microscope
Provider: Core Facility for Electron Microscopy
Affiliations: Charite
Description: Suitable for standard ultrastructural analysis.
Name: AMBIO Nikon Scanning Confocal A1Rsi+
Resource type: Microscope
Provider: AMBIO
Affiliations: Charite
Description:
Imaging modes
- Scanning confocal (DAPI, GFP, mCherry, Cy5)
- FRAP/PA (photo activation/conversion)
- Live-cell incubator (temperature, CO2, humidity)
- Spectral scans
- FLIM/FCS
Lasers and max. intensities out of 60x oil objective
- 405 nm : 0.44 mW
- 488 nm : 0.80 mW
- 561 nm : 1.66 mW
- 647 nm : 1.70 mW
Emission Filters
Filter | λ/Bandwidth |
DAPI | 450/50 |
GFP | 525/50 |
mCherry | 595/50 |
Cy5 | 700/75 |
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 512x512 pixels, up to 420 fps in 512x32 pixels)
- Pinhole: hexagonal, tunable
Detectors
- 2x Hybrid GaAsP PMT (high sensitivity, GFP, mCherry)
- 2x PMT (Dapi, Cy5)
- Transmission light detector (DIC, phase)
- Spectral detector (optical diffraction grid and 32-channel multianodes)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x | Plan Achroat | Air | 0.3 | 10,500 | |
20x | Plan Apo | Air | 0.8 | 1,000 | DIC N2 VC |
40x | Apo | Water | 1.25 | 180 | DIC N2 λ S |
60x | Plan Apo | Water | 1.27 | 170 | DIC N2 IR |
40x | Plan Flour | Oil | 1.3 | 200 | DIC N2 H |
60x | Plan Apo | Oil | 1.4 | 130 | λ |
Special
- Fast switch between galvo and resonant scanners
- Spectral scanner (unmixing)
Comments: New users - access upon training request here.
Name: AMBIO Nikon Spinning Disk Confocal CSU-X
Resource type: Microscope
Provider: AMBIO
Affiliations: Charite
Description:
Imaging modes
- Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
- FRAP/ PA (photo activation/ conversion)
- Live-cell incubator (temperature, CO2, humidity)
Lasers and max. intensities out of 60x oil objective
- 405 nm : 3.54 mW
- 488 nm : 3.85 mW
- 561 nm : 4.11 mW
- 647 nm : 4.68 mW
Emission Filters
Filter | λ/Bandwidth |
Quad emission filter (DAPI, GFP, mCherry, Cy5) | 440/40 521/21 607/34 700/45 |
DAPI | 452/45 |
GFP | 525/50 |
mCherry | 600/50 |
Cy5 | 685/40 |
GFP narrow | 510/20 |
Detectors
- 2 EMCCD cameras (iXon3 DU-888 Ultra, 1024x1024 pixels, 13 μm pixel size, 26 fps full frame, usable area ~512x512 pixels, 93 fps)
- Current dual color dichroic for simultaneous GFP & mCherry detection
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x (not installed) | Plan Achroat | Air | 0.3 | 10,500 | |
20x | Plan Apo | Air | 0.8 | 1,000 | DIC N2 VC |
40x | Apo | Water | 1.25 | 180 | DIC N2 λ S |
60x | Plan Apo | Water | 1.27 | 170 | DIC N2 IR |
40x | Plan Flour | Oil | 1.3 | 200 | DIC N2 H |
60x | Plan Apo | Oil | 1.4 | 130 | λ |
100x | Plan Apo | Oil | 1.45 | 130 | λ |
Special
- Andor Revolution SD System (CSU-X)
- Uniform spinning disk illumination
- 2 cameras for ultra-fast dual color imaging
- Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
- Ultra-fast laser switching/ tuning (triggered acquisition)
Comments: New users - access upon training request here.
Name: AMBIO Nikon Widefield Ti2
Resource type: Microscope
Provider: AMBIO
Affiliations: Charite
Description:
Imaging modes
- Widefield EPI fluorescence (DAPI, CFP, GFP, YFP, mCherry, Cy5)
- Bright-field for histology slides
- Automated phase for 10x, 20x objectives
- Live-cell incubator (temperature, CO2, humidity)
- Multiplexing (multi position, large image scan, automation)
LED Illumination (Lumencore, SpectraX) and max. intensities out of 40x objective
- 395/25 nm : 66.5 mW
- 438/29 nm : 109 mW
- 475/28 nm : 57.0 mW
- 511/16 nm : 12.3 mW
- 555/28 nm : 145 mW
- 635/22 nm : 38.9 mW
Emission Filters
Filter | λ/Bandwidth |
Quad emission filter (DAPI, GFP, mCherry, Cy5) | 435/33 520/26 595/38 695/63 |
CFP | 473/24 |
YFP | 540/30 |
GFP | 519/26 |
mCherry | 595/31 |
Cy5 | 697/60 |
Detectors
- sCMOS, PCO.edge camera (>80% QE, 2048x2048 pixels, 6.5 µm pixel size, 53 fps full frame, up to 500 fps in small area)
- Nikon DS-Ri2 color CMOS camera (4908x3264 pixels, 6 fps full frame, 45 fps in 1636x1088 pixels)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
4x | Plan Apo | Air | 0.2 | 20,000 | λ |
10x | Plan Flour | Air | 0.3 | 15,200 | Ph1 DL |
20x | Plan Apo | Air | 0.8 | 1,000 | Ph2 DM λ |
40x | Apo Flour | Air | 0.95 | 250 | λ |
Special
- Fast LED laser switching/ tuning (triggered acquisition)
- Fast emission filter wheel for clean multi-color imaging of DAPI, CFP, GFP, YFP, mCherry, and Cy5
Comments: New users - access upon training request here.
Name: AMBIO Nikon Multiphoton Confocal
Resource type: Microscope
Provider: AMBIO
Affiliations: Charite
Description:
Imaging modes
- Multiphoton confocal
- DIC, relief contrast
Laser (Mai Tai Insight Deepsee Dual)
- Tunable: 680 nm (600 mW) – 900 nm (500 mW) – 1300 nm (500 mW)
- Single line: 1040 nm
Emission Filters
Filter | λ/Bandwidth |
DAPI | - |
GFP | 525/50 |
mCherry | 595/50 |
Cy5 | 700/75 |
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 512x512 pixels, up to 420 fps in 512x32 pixels)
Non-Descanned Detectors (no pinhole)
- 2x GaAsP-NDD (highly sensitive, GFP, mCherry)
- 2x PMT-NDD (DAPI, Cy5)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
25x | Apo | Water | 1.1 | 2,000 | NCC |
40x | Apo | Water | 0.8 | 3,500 | NIR |
Special
- Upright
- Extended IR laser for deep tissue imaging
- Fast switch between glavo and resonant scanner
Comments: New users - access upon training request here.
Name: AMBIO Nikon TIRF/STORM
Resource type: Microscope
Provider: AMBIO
Affiliations: Charite
Description:
Imaging modes
- Widefield EPI fluorescence (DAPI, GFP, mCherry, Cy5)
- Phase (10x, 20x)
- DIC (20x, 40x, 100x)
- TIRF (adhesive cell membrane), motorized TIRF angle
- dSTORM (super-resolution, single molecule localization)
- FRAP/ PA (photo activation/ conversion)
- Live-cell incubator (temperature, CO2, humidity)
Lasers (max. intensities out of 100x objective)
- 405 nm : 3.31 mW
- 488 nm : 53.5 mW
- 561 nm : 37.4 mW
- 647 nm : 80.7 mW
Emission filters
High speed emission filter wheel (OptoSpin IV) for clean multi-color imaging:
Filter | λ/Bandwidth |
GFP | 525/50 |
mCherry | 600/37 |
Cy5 | 635 LP |
Emission splitter (OptoSplit III) for simultaneous 2- or 3-color imaging:
SD-dSTORM cube | |
AF568, mCherry | |
AF647, CF680 | |
Triple cube | |
GFP, AF488 (525/50) | |
AF568, mCherry (600/37) | |
AF647, CF680 (700/75) |
Detectors
- Camera 1 (left port): EMCCD, Andor iXon Ultra X3 987, 512x512 pixels, 16 µm pixel size, 56 fps full frame, QE max. 90%
- Camera 2 (right port): sCMOS, Photometrics Prime 95B (95% QE, 1200x1200 pixels, 11 µm pixel size, 41 fps full frame, 82 fps@12-bit)
- Camera 3 (left port front): CMOS, The Imaging Source DMK 23UX249 (1920x1200 pixels, 5.86 µm pixel size, 30 fps full frame)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x | Plan Flour | Air | 0.3 | 15,200 | Ph1 DL |
40x | Plan Flour | Oil | 1.3 | 240 | DIC H N2 |
60x | Plan Apo | Oil | 1.4 | 130 | λ |
100x | Plan Apo | Oil | 1.5 | 120 | DIC H N2 TIRF |
(All objectives have an external phase contrast)
Special
- 2x beam condenser for small FOV (dSTORM)
- Ultra-fast laser switching/ tuning (triggered acquisition)
Comments: New users - access upon training request here.
Name: AMBIO Nikon Upright Spinning Disk Confocal S2
Resource type: Microscope
Provider: AMBIO
Affiliations: Charite
Description:
Imaging modes
- Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
- Upright
Lasers and max. intensities out of 25x objective
- 405 nm : 6.3 mW
- 488 nm : 7.7 mW
- 561 nm : 9.7 mW
- 647 nm : 9.5 mW
Detectors
- Zyla 4.2 Plus sCMOS camera (2048x2048 pixels, 40 fps@16-bit, 53 fps@12-bit)
Emission Filters
Filter | λ/Bandwidth |
Quad emission filter (DAPI, GFP, mCherry, Cy5) | 440/20 521/20 607/20 700/20 |
DAPI | 447/60 |
GFP | 525/25 |
mCherry | 600/52 |
Cy5 | 708/75 |
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x | Plan Achroat | Water | 0.45 | 4,000 | λ |
25x | Apo | water | 1.10 | 2,000 | NCC |
Special
- Andor Revolution SD System (CSU-X1)
- Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
- Ultra-fast laser switching/ tuning (triggered acquisition)
Comments: New users - access upon training request here.
Name: 4. Ask a question to admin
Resource type: Administration
Provider: Administration Open IRIS Charité & BIH
Affiliations: Charite, BIH
Description: You can ask a question to the OpenIRIS coordinator regarding all matters OpenIRIS.
Name: Ultimaker S5
Resource type: 3D Printer
Provider: Central Scientific Workshop
Affiliations: Charite
Description: Discover the easy-to-use desktop 3D printer with a large build volume
that delivers accurate, industrial-grade parts, time and again. With
simple setup, high uptime, and reliable dual extrusion, Ultimaker S5 is
the complete professional 3D printing solution.
Fused deposition modeling (FDM) for PLA, ABS, PC filaments and others.
Schmelzschichtung (FDM) für PLA, ABS, PC-Filament und andere.
Comments: Only operated by facility staff.
Name: Device 1 | PharmaScan 70/16 US
Resource type: Magnetic Resonance Imaging System
Provider: 7T Experimental MRIs
Affiliations: Charite
Description: Device 1 | PharmaScan 70/16 US (installation 03/2003; upgrade 04/2013)
Bruker Software Paravision 5.1
Comments: Access to the equipment is only granted upon a service request (see services here).
Bookings have to be approved by the facility (indicated by '-'). You will receive an email once your booking is approved.
Name: Device 2 | BioSpec 70/20 USR
Resource type: Magnetic Resonance Imaging System
Provider: 7T Experimental MRIs
Affiliations: Charite
Description: Device 2| BioSpec 70/20 USR (installation 08/2013)
Bruker Software Paravision 6.0.1
Comments: Access to the equipment is only granted upon a service request (see services here).
Bookings have to be approved by the facility (indicated by '-'). You will receive an email once your booking is approved.
Name: Formlabs Form 2
Resource type: 3D Printer
Provider: Central Scientific Workshop
Affiliations: Charite
Description: The Form 2 delivers high-resolution parts at a fraction of the cost and
footprint of industrial 3D printers. Scale your prototyping and
production in-house with desktop stereolithography.
Stereolitography (SLA) for modern synthetic resins.
Stereolithografie (SLA) für moderne Kunstharze.
Comments: Only operated by facility staff.
Name: Markforged Onyx Pro
Resource type: 3D Printer
Provider: Central Scientific Workshop
Affiliations: Charite
Description: The Onyx Pro prints composite parts that are
10x the strength of plastic by reinforcing chopped-carbon nylon with
continuous strands of fiberglass.
Suitable for nylon with carbon fiber and optionally glass fiber.
Geeignet für Nylon mit Karbon-Fasern und optional Glasfaser.
Comments: Only operated by facility staff.
Name: CO2 Laser Cutter SH-G6090 150W (Sabko GmbH)
Resource type: Laser Cutter
Provider: Central Scientific Workshop
Affiliations: Charite
Description: CO2 Laser Cutter SH-G6090 150W (Sabko GmbH)
Suitable for acrylic glass and POM.
Geeignet für Acryl-Glas und POM.
Comments: Only operated by facility staff.
Name: Project Meetings - Imaging Mass Cytometry (Hyperion)
Resource type: Project Discussion
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description:
Imaging Mass Cytometry (IMC) is an approach for the targeted and epitope-specific multiparameter analysis of tissue sections (Scheme). In contrast to conventional immunohistochemistry or immunofluorescence, this technology allows for the simultaneous detection of >35 specific markers on the tissue by using metal-isotopes and their different masses as analytes. Tissue sections are labeled with epitope-specific, metal isotope conjugated antibodies and the tissue is ablated in 1x1 µm spots by a laser. The ablated material is carried into the mass cytometer, and the metal ion content of each pixel is analyzed by time-of-flight mass spectrometry. The signals are then plotted as a multiparameter image that can be analyzed by any imaging software. For more information about this new technology contact the core facility.
Measurement of tissue samples
IMC is offered as service (staff-operated) or independent usage with assistance. A project meeting is required to get access. We will discuss your experimental setup, technical details and the timeline for your sample processing. Please request a project meeting as early as possible, but latest 6 weeks before onset of your experiment.
Comments:
Please download the project registration form and the table for your first panel draft and attach it to the request.
Name: Hyperion - Imaging Mass Cytometer
Resource type: Imaging Mass Cytometer
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description:
Imaging Mass Cytometry (IMC) is an approach for the targeted and epitope-specific multiparameter analysis of tissue sections (Scheme). In contrast to conventional immunohistochemistry or immunofluorescence, this technology allows for the simultaneous detection of >35 specific markers on the tissue by using metal-isotopes and their different masses as analytes. Tissue sections are labeled with epitope-specific, metal isotope conjugated antibodies and the tissue is ablated in 1x1 µm spots by a laser. The ablated material is carried into the mass cytometer, and the metal ion content of each pixel is analyzed by time-of-flight mass spectrometry. The signals are then plotted as a multiparameter image that can be analyzed by any imaging software. For more information about this new technology contact the core facility.
Measurement of tissue samples
IMC is offered as service (staff-operated) or independent usage with assistance. Measurement of tissue sections can be requested via OpenIRIS ('Services' - 'Imaging Mass Cytometry - Sample Acquisition') with a date/deadline and will then be coordinated by the core facility. Longer measurements usually run overnight or over the weekend. Please request measurements as early as possible.
Comments:
Usage of imaging mass cytometry can be requested in "services".
Check CyTOF Scheduler for your bookings and available slots.
Please contact the core facility at least 6 weeks before starting new projects to discuss technical details and instrument availability.
Name: Project Meetings - Flow Cytometry
Resource type: Project Discussion
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description:
Comments:
Project discussion before use of analysers and cell sorting service.
Please download the registration form and attach it to the request.
Name: Project Meetings - Mass Cytometry
Resource type: Project Discussion
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description:
Comments:
Please download the project registration form and CyTOF panel template and attach it to the request.
Name: Imaging Mass Cytometry - Sample Acquisition
Resource type: Imaging Mass Cytometry
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description:
Imaging Mass Cytometry (IMC) is an approach for the targeted and epitope-specific multiparameter analysis of tissue sections (Scheme). In contrast to conventional immunohistochemistry or immunofluorescence, this technology allows for the simultaneous detection of >35 specific markers on the tissue by using metal-isotopes and their different masses as analytes. Tissue sections are labeled with epitope-specific, metal isotope conjugated antibodies and the tissue is ablated in 1x1 µm spots by a laser. The ablated material is carried into the mass cytometer, and the metal ion content of each pixel is analyzed by time-of-flight mass spectrometry. The signals are then plotted as a multiparameter image that can be analyzed by any imaging software. For more information about this new technology contact the core facility.
Measurement of tissue samples
IMC is offered as service (staff-operated) or independent usage with assistance. Measurement of tissue sections can be requested via OpenIRIS ('Services' - 'Imaging Mass Cytometry - Sample Acquisition') with a date/deadline and will then be coordinated by the core facility. Longer measurements usually run overnight or over the weekend. Please request measurements as early as possible.
Comments: Please request a project meeting at least 6 weeks before start of a new project to get access.
The sample acquisition template -> new version!! (download) has to be uploaded when submitting the request and a description of the slide has to be provided (see example here).
Name: nanoSPECT/CT plus
Resource type: Preclinical Imaging System
Provider: BERIC
Affiliations: Charite
Description:
- Resolution-booster™: the first ground breaking total detection chain design principle
- Patented* M3-pinhole™ and Tera-Tomo™ 3D SPECT reconstruction technology
- x2 gain in SPECT sensitivity achieve 13.000 cps/MBq
- x2 gain in volumetric SPECT resolution
Comments: If you would like to use the instrument, please contact us via email (see contacts) or phone (+49 (0)30 450 557013).
Name: nanoScan PET/CT
Resource type: Preclinical Imaging System
Provider: BERIC
Affiliations: Charite
Description:
Comments: If you would like to use the instrument, please contact us via email (see contacts) or phone (+49 (0)30 450 557013).
Name: Titan Krios
Resource type: TEM Microscope
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: According to the procurement
regulations of the DFG, access to the Titan
Krios is primarily reserved to the group of applicants of the 91b initiative until the end of 2024.
You can request access to single particle data acquisition on the Titan Krios microscope via the services 'Participate in SPA data acquisition (FU-Berlin)'.
After confirmation, you can request beam time for SPA via the service ´SPA data acquisition (FU Berlin)´.
Comments:
There is no direct access to this microscope!
You can request assisted and unassisted SPA measurements at the Titan Krios microscope.
Click the magnifying glass on the left for more details.
Name: FEI Vitrobot Mark IV (FZEM)
Resource type: Freeze Plunger
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Booking of the Vitrobot is restricted to the group of TEM users only. If you are not member of this group please contact the resource admin(s).
Unassisted preparations are reserved to skilled (trained) users, otherwise a qualified operator is mandatory.
Training for unassisted operations can be requested from the timeline view in the scheduler (top right, booklet symbol). You will be informed after submitting a training request.
Due to the pandemic training is not offered at the moment.
Comments:
Please note the restricted access to this instrument!
Click the magnifying glass on the left for more details.
! Bookings become valid only after approval by the staff !
Name: Talos L120C
Resource type: TEM Microscope
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Booking of the Talos L120C (conventional and cryo EM) is restricted to registered members of the BioSupraMol core facillity. Registration can be requested via the Service:
´Register to TEM (BSM)´.
After registration, booking is possible in 4 hour time slots (9:00 to 13:00 and 13:00 to 17:00) from Monday to Friday. (Rare exceptions are matter of agreement.) Unassisted measurements are reserved to skilled (trained) users, otherwise a qualified operator is mandatory and can be chosen during the booking process. It is best practice to make an appointment with the operators before booking assisted measurements.
Training for unassisted microscope operation can be requested from the timeline view in the scheduler (top right, booklet symbol). You will be informed after submitting the training request.
Comments:
Please note the restricted access to this microscope!
Bookings are only possible from Monday to Friday at two blocks (9:00 to 13:00 and 13:00 to 17:00). Click the magnifying glass on the left for more details.
! Bookings become valid only after approval by the staff !
Name: SPA data acquisition - Titan Krios (FZEM - SFAB)
Resource type: Service
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Beam time on the Titan Krios microscope is only accesible by this service request.
Every request demands a brief description of the sample and the preliminar work, and must be approved by the group head of the requester. Only after this confirmation, the service can be initiated. We will then, together with the requester, decide on the
necessary steps prior to the data acquisition.
currently, booking of the microscope is based on the usage policies of the Core Facility BioSupraMol.
Comments:
Please note the restricted access to the SPA workflow!
This request for SPA data acquisition on the Titan Krios 300 kV electron microscope is only possible for registered users.
You can request a registration via the 'Register for Single Particle Analysis (SPA) - SFAB' request.
Name: Talos Arctica
Resource type: TEM Microscope
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description:
Comments:
Direct access is restricted to members of the FZEM!
Measurements and beam times are accessible via the services:
'TEM measurements (FZEM - BSM)´.
Name: Incucyte DKTK CBF
Resource type: Cell Analyzer
Provider: AG Siegmund
Affiliations: Charite
Description: Satorius Live Cell analyzer
Comments:
Everyone can use it, but DKTK members have precedence. If you're a new user, please contact "rainer.glauben@charite.de" for an introduction.
Name: Participate in SPA data acquisition - SFAB
Resource type: Administration (BSM/SFAB - FU)
Provider: Administration OpenIRIS@FUB (BCP)
Affiliations: FU Berlin
Description: According to the procurement regulations of the DFG, access to the Titan Krios is primarily reserved to the group of applicants of the 91b initiative until the end of 2024.
Comments:
This request addresses to group heads and PI's only
Requesting admission to the SPA workflow enables your group members to register for the SPA data acquisition service at FZEM. Until the end of 2024, the SPA workflow is primarily reserved to 91b Titan Krios applicants.
Members of registered groups must, however, only use the 'Register to Single Particle Analysis (SPA) - SFAB' request to get access.
Name: Project request
Resource type: Project
Provider: High-Throughput Mass Spectrometry
Affiliations: Charite
Description: Please request an initial meeting at least 4 weeks before the start of a new project.
Name: ThermoFisher Vitrobot Mark IV
Resource type: Plunge Freezer
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Key Benefits:
- Fully automated, reproducible vitrification of aqueous suspensions
- Precise control of critical process parameters
- Enclosed process chamber
- High sample throughput
- Easy and flexible user interface
- Semi-automated grid transfer
Name: ThermoFisher Titan Krios G3i
Resource type: cryo-Transmission Electron Microscope
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Key Features:
- 80 - 300 keV Acceleration Voltage
- X-FEG Electron Source
- Twelve cartridge Auto Loading System
- Ceta 16Mpx CMOS Camera
- Falcon 3 DED
- GATAN K3 DED + BioQuantum GIF
- Volta Phase Plate
- Dual Axis Tomography Holder
- Software Packages: EPU, Tomo5, SerialEM, Maps
- Live pre-processing of raw data
Name: ThermoFisher Aquilos
Resource type: cryo-FIB/SEM
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Key Features:
- Complete cryo infrastructure including cryo transfer station
- Fully rotatable cryo-stage
- Special Cryo-FIB Autogrids for shallow-angle milling
- Sample shuttle for Autogrids: Cryo-FIB shuttle with integrated shutter system during cryo-transfers
- In-chamber retractable sputter coater for applying conductive coatings
- GIS-system for applying protective coatings
- Including MAPS software for correlative workflow
Name: LEICA TCS SP8
Resource type: cryoCLEM
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Key Features:
- Complete cryo Infrastructure including cryo transfer station
- HCX PL APO 50x CLEM cryo-Objektive
- SP8 confocal optical system + SP8 LIGHTNING software
- Compatible with MAPS software for correlative workflow
Laser Lines
405,488,552,638
Detectors
1x PMT detector, 2x HyD detectors
Name: 3T MRI system MRS 3017
Resource type: Magnetic Resonance Imaging System
Provider: BERIC
Affiliations: Charite
Description: The 3T Benchtop MRI (MRsolutions MRS 3017; date of manufacture 2015) has a high-performance gradient system and a comprehensive selection of coils. The system is suitable for both cardiac and non-cardiac imaging studies in mice and rats and is compatible with multinuclear imaging (fluorine, xenon, etc.).
Comments: If you would like to use the instrument, please contact us via email (see contacts) or phone (+49 (0)30 450 557013).
Name: Luminescent Image Analyzer
Resource type: Equipment
Provider: AG Siegmund
Affiliations: Charite
Description:
Comments: everyone can use it
Name: Real-Time PCR Machine (StepOne)
Resource type: qPCR
Provider: AG Siegmund
Affiliations: Charite
Description:
Comments: everyone can use it
Name: Aurora "Rory" - Full Spectrum Analyzer
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description:
Comments:
You need to be registered and trained to use this instrument.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Name: SU 8030
Resource type: SEM Microscope
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Booking of the SU8030 (cryo and conventional SEM) is restricted to registered users. Registration can be requested via the service:
´Register to SEM (BSM)´.
After registraition, booking times are are available on the hour from 8:00 to 18:00 (Fri 17:00) in blocks of 60 minutes only. Durations can range from 1 hr up to a maximum of 10 (9) hrs. (Rare exceptions are matter of agreement.) Unassisted measurements are reserved to skilled (trained) users, otherwise a qualified operator is mandatory, which may be chosen during the booking process. It is best practice to make an appointment with the operators before booking assisted measurements.
Training for unassisted microscope operation can be requested from the timeline view in the scheduler (top right, booklet symbol). You will be informed after submitting a training request.
Comments:
Please note the restricted access to this microscope!
Booking times are on the hour from 8:00 to 18:00 (Fri 17:00) in blocks of 60 minutes only. Click the magnifying glass on the left for more details.
! Bookings become valid only after approval by the staff !
Name: Leica DMi8
Resource type: Light microscopy
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description:
Inverted microscope for Live-cell imaging
- Incubation chamber for Temperature and CO2 control
- Adaptive Focus Control (AFC)
- DFC9000 GT Camera
- Available to use with the PRIMO device
Name: PRIMO Alveole for micropatterning
Resource type: Other Instruments
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description:
A device for well-positioned cells within the mesh of the EM grids, and several other applications.
- protein micropatterning
- hydrogel structuration
- microfabrication
Please feel free to contact us for further information on the technique and applications.
Name: FIB-milling with Aquilos2 Training
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Obligatory training for all users who wish to use the Aquilos microscope
Name: PRIMO training
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Obligatory training for all users who want to use the PRIMO micropatterning device
Name: Training for Vitrobot
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Obligatory training for all users who want to use the Vitrobot machine.
Name: Training for cryoCLEM microscopy
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Obligatory training for all users who want to use the cryo confocal SP8
Name: Training on cryo TEM (Krios)
Resource type: Training
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Obligatory training for all users who want to use the Titan Krios
Name: Graphics Workstation
Resource type: Workstation
Provider: Core Facility for Cryo-Electron Microscopy
Affiliations: Charite
Description: Windows GPU workstation
Name: User Registration
Resource type: Registration
Provider: BIH Core Unit pluripotent Stem Cells & Organoids
Affiliations: Charite, BIH
Description: In order to use the services and infrastructure of the BIH Core Facility Stem Cells, users must first register and accept the user regulations. The registration requires the confirmation of the respective group leader. The group leader receives a notification in OpenIRIS and can confirm it here.
Name: Symphony A3 "Polyhymnia"
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description:
5-Laser Flow Cytometry Analyser
Please request a project meeting and upload the registration form for your sample material to get access.
A training is required for independent usage.
Instrument Configuration (download)
Comments:
You need to be registered and trained to use this instrument.
For analysis of GMO material contact the BIH CCF staff to discuss formalities.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Name: Cell Sorter - AriaFusion "Terpsichore"
Resource type: Flow Cytometry Sorter
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description:
4-Laser Sorter, equipped with 15 fluorescence parameters.
Cells can be sorted in bulk (up to 4 populations simultanously) or as single cells in 96well plates / 384well plates. Index Sorting is available.
Please request a project meeting and upload a registration form for your sample material to get access to cell sorting.
Instrument Configuration (download)
Comments:
Please request a project meeting to get access to cell sorting services.
NEW!! Please book your slot and wait for approval. Your booking is not guaranteed until you get notification of approval.
Cancel your booking 48 hours ahead to avoid charges.
For ad-hoc, short notice bookings (next 48 hours) please contact us directly.
Please note: UV-Laser is no longer available on this instrument. See updated configuration here.
Name: Eastwood Computer
Resource type: Computer
Provider: AG Siegmund
Affiliations: Charite
Description: everyone can use it
Comments: everyone can use it
Name: Seahorse
Resource type: Cell Analyzer
Provider: AG Siegmund
Affiliations: Charite
Description: everyone can use it
Comments: everyone can use it
Name: OMERO User Access Request (Charité/BIH only)
Resource type: Training
Provider: OMERO Microscopy Image Data Management
Affiliations: Charite, BIH
Description: Request for new users to gain access to the OMERO image repository system (for users internal to Charité/BIH).
Comments: Please submit access request to get an account on OMERO+ (for users internal to Charité/BIH).
Name: OMERO Training course sign-up
Resource type: Request
Provider: OMERO Microscopy Image Data Management
Affiliations: Charite, BIH
Description: OMERO training sessions from basics on how to get started with OMERO to more advanced usage topics.
Name: TEM measurements (FZEM - BSM)
Resource type: Service
Provider: Electron microscopy unit - BCP
Affiliations: FU Berlin
Description: Service to performing TEM measurements (including phase plate, automated data acquisition, and electron tomography) at the Forschungszentrum für Elektronenmikroskopie (FZEM). Decisions on the requests are made by the staff at the FZEM. The requester will be informed of the
decision.
Skilled (trained) users can perform non-assisted measurements, otherwise a qualified operator is mandatory, which can be recommended in the request.
Booking of this service is based on the usage policies of the BioSupraMol core facility.
Comments:
Service to request assisted and unassisted TEM measurements at the FZEM. Approvals of the request by your group head may be required.
Name: STEM Nion HERMES 200 kV
Resource type: Transmission Electron Microscope
Provider: BeAM - Berlin-Adlershof Center for Advanced Microscopy
Affiliations: HU Berlin, FHI, HZB, IKZ
Description:
- monochromated (energy resolution < 6 meV)
- aberration-corrected (probe size at 200 kV < 0.07 nm)
- side entry stage (double-tilt cryo holder with electrical contacts, etc.)
- direct detector for EELS and zero-loss filtered diffraction (Dectris)
- sample in ultra-high vacuum
Name: TEM/STEM JEOL JEM2200FS
Resource type: Transmission Electron Microscope
Provider: BeAM - Berlin-Adlershof Center for Advanced Microscopy
Affiliations: HU Berlin, FHI, HZB, IKZ
Description:
- 200 kV, field emission gun
- UHR pole piece
- 1k slow-scan CCD camera (Gatan)
- ASTAR system (NanoMegas) including Beam precession unit, SNBD system, ACOM software
- BF STEM detector
- HAADF STEM detector
- Electron biprism
- Digiscan system (Gatan)
- In-column energy filter
- LN2 free energy dispersive X-ray SD detector (Bruker)
Name: Course on human induced pluripotent Stem Cells (iPSCs) - 20-22 March 2024
Resource type: Training
Provider: BIH Core Unit pluripotent Stem Cells & Organoids
Affiliations: Charite, BIH
Description:
Working with human pluripotent stem cells (hPSC), including generation, differentiation as well as establishment of disease models requires a profound knowledge and hands-on expertise.
The processes involved in these aspects of hPSC handling in tissue culture are labor intensive and distinct from that of other mammalian cell cultures. The BIH Core Unit pluripotent Stem Cells & Organoids (CUSCO) and the MDC Technology Platform pluripotent Stem Cells are jointly offering training on these methodologies.
The lectures and practical training will cover all areas of hPSC technology including techniques and practical advice for maintenance, characterization & QC, differentiation and genetic manipulation.
The lectures and lab training will be at the Charité, Campus Virchow-Klinikum. Lectures will be held in a hybrid format.
When?
20-22 March 2024
Who should attend this course?
The training is intended for researchers and technicians who are planning projects using hPSCs and/or will start working with hPSCs in the near future.
The hands-on part of the training is limited to eight attendees to provide a comfortable learning experience and allow for personalized interactions between the trainers and attendees. The lectures are not limited in attendees and offered in a hybrid format.
Prerequisite
Previous hands-on experience in mammalian cell culture, familiarity with basic molecular and cell biology principles and methods are mandatory. The course is open to BIH, Charité and MDC investigators.
Registration
Please apply until 1 March 2024.
The course is free of charge!
For any questions, please contact Dr. Harald Stachelscheid (harald.stachelscheid@bih-charite.de) or Dr. Sebastian Diecke (Sebastian.diecke@mdc-berlin.de).
Comments:
20-22 March 2024
Name: Oxford Diffraction Xcalibur Nova O
Resource type: X-ray Diffractometer
Provider: X-Ray diffraction unit - BCP
Affiliations: FU Berlin
Description: This Oxford Diffraction Xcalibur Nova O single crystal diffractometer is available for non-assisted measurements for trained persons from FUB. It is equipped a Cu x-ray sources, an Onyx CCD detector, and an Oxford CryojetXL.
Comments: Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible in three predetermined blocks.
Name: Project consultation
Resource type: Consultation
Provider: BIH Core Unit pluripotent Stem Cells & Organoids
Affiliations: Charite, BIH
Description:
To allow sufficient time for all our users and be able to continue developing Core Projects we ask that ALL the meetings to be scheduled in advance through this service request. Please send us a request for a meeting indicating who you want to meet and with suggested time and we will respond promptly with our availability.
These include:
- Experiment/Project planning
- Help with experiment execution/ consultation
- Equipment help
- Updates/results discussions
For questions and requests regarding consumables (media, plasticware, chemicals, etc.), cell lines and standard protocols, please contact the technicians.
Name: Cell Sorter - Aurora CS "Luke" - Full Spectrum Sorter
Resource type: Flow Cytometry Sorter
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description: Full Spectrum Cell Sorter
Comments:
Please request a project meeting to get access to cell sorting services.
NEW!! Please book your slot and wait for approval. Your booking is not guaranteed until you get notification of approval.
Cancel your booking 48 hours ahead to avoid charges.
For ad-hoc, short notice bookings (next 48 hours) please contact us directly.
Name: Seminar Room 1504 (Familienzimmer)
Resource type: Room
Provider: ECRC Seminar Rooms
Affiliations: MDC, Charite
Description: Seminar Room 35m^2 (for 10-15 people)
Comments:
This room is locked. Upon booking via OpenIRIS, the keys are available at the reception in the KBH (house 51/Lindenberger Weg 80).
Dieser Raum ist verschlossen. Nach der Buchung via OpenIRIS sind die Schlüssel an der Rezeption im KBH (Haus 51/Lindenberger Weg 80) erhältlich.
Name: Seminar Room 3314 (Röntgendemoraum)
Resource type: Room
Provider: ECRC Seminar Rooms
Affiliations: MDC, Charite
Description:
Comments:
Capacity: 40- 60 chairs; Kapazität: 40-60 Stühle
This room is locked. Upon booking via OpenIRIS, the keys are available at the reception in the KBH (house 51/Lindenberger Weg 80).
Dieser Raum ist verschlossen. Nach der Buchung via OpenIRIS sind die Schlüssel an der Rezeption im KBH (Haus 51/Lindenberger Weg 80) erhältlich.
Name: Seminar Room 2312
Resource type: Room
Provider: ECRC Seminar Rooms
Affiliations: MDC, Charite
Description: Seminar Room 35m^2 (for 10-15 people)
Comments:
Capacity: 10-15 chairs; Kapazität:10-15 Stühle
This room is locked. Upon booking via OpenIRIS, the keys are available at the reception in the KBH (house 51/Lindenberger Weg 80).
Dieser Raum ist verschlossen. Nach der Buchung via OpenIRIS sind die Schlüssel an der Rezeption im KBH (Haus 51/Lindenberger Weg 80) erhältlich.
Name: Zeiss GeminiSEM 500
Resource type: Scanning Electron Microscope
Provider: BeAM - Berlin-Adlershof Center for Advanced Microscopy
Affiliations: HU Berlin, FHI, HZB, IKZ
Description:
- NanoVP Option
- In-lens SE detector
- In-lens EsB detector
- BSE 4-Quandrant detector
- STEM detector
- Bruker XFlash 6|30 EDX system
- Raith ELPHY Quantum Lithography system
- costum stages for 4D-STEM
Name: Aurora "Lane" - Full Spectrum Analyzer
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description:
For more information on full spectrum flow cytometry see:
https://cytekbio.com/blogs/videos#tab-tutorials
https://cytekbio.com/pages/aurora
Comments:
You need to be registered and trained to use this instrument.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Name: SR 101 - seminar room (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Small seminar room with tables and 16 seats, equipped with electronic smartboard (mobile). Upgrade with laptop and video camera system is possible. (Webex-)Videomeetings with own laptop possible.
Comments: 16 seats, with tables, equipped with electronic smartboard (mobile).
Recurring bookings are possible; please remember to define the end date!
Laptop and video system can be chosen as add-on throughout the booking procedure.
! Bookings become valid only after approval by the staff !
Name: SR 011 - seminar room (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Small seminar room with tables and 16 seats, equipped with electronic smartboard (mobile) and analog whiteboard (fixed). Upgrade with laptop and video camera system is possible. (Webex-)Videomeetings with own laptop possible.
Comments: 16 seats, with tables, equipped with electronic smartboard (mobile) and analog whiteboard (fixed).
Recurring bookings are possible; please remember to define the end date!
Laptop and video system can be chosen as add-on throughout the booking procedure.
! Bookings become valid only after approval by the staff !
Name: SR 119 - large seminar room (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Large seminar room without tables, about 100 seats, equipped with a beamer and analog whiteboard (fixed).
Comments: 84 seats, without tables, equipped with a beamer and fixed whiteboard (analog).
Recurring bookings are possible; please remember to define the end date!
! Bookings become valid only after approval by the staff !
Name: Small meeting point area / Area in front of kitchen (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Area in front of the large kitchen for small tables / poster area, about 12 couch seats.
Comments: 12 couch seats, area in front of large kitchen level 2.
Recurring bookings are possible; please remember to define the end date!
! Bookings become valid only after approval by the staff !
Name: Add my group to FUB IRIS - FU-Berlin
Resource type: Administration (BSM/SFAB - FU)
Provider: Administration OpenIRIS@FUB (BCP)
Affiliations: FU Berlin
Description:
A group is necessary to assign users to a real working group which can adopt the measurement charges. Therefore, only users assigned to a group are able to book resources.
After installation of your group is finished, please do appoint a second group admin that can take over administrative tasks for the group in OpenIRIS.
Comments:
This request addresses to group heads only.
Adding your group to FUB IRIS by this request is restricted to groups of the department of Biology, Chemistry, and Pharmacy (BCP), the department of Physics and the department of Veterinary Medicine.
Name: AMBIO Nikon SoRa Spinning Disk Confocal CSU-W1
Resource type: Microscope
Provider: AMBIO
Affiliations: Charite
Description:
Imaging Modes
- Spinning disk confocal (DAPI, CFP, GFP, YFP, mCherry, TxRED, Cy5)
- SoRa-mode (Super Resolution by Optical Pixel Reassignment, 1.4x resolution improvement)
- FRAP/ PA (photo activation/ conversion)
- Widefield EPI fluorescence mode (DAPI, GFP, TxRED, TRITC-B, Cy5)
- DIC for 10x, 40x, 60x, 100x objectives (upon request)
- Live-cell incubator (temperature, CO2, humidity)
7 Laser Lines
- 405 nm
- 445 nm
- 488 nm
- 515 nm
- 561 nm
- 594 nm
- 638 nm
Detectors
- 2x Hamamatsu ORCA-Fusion digital sCMOS cameras (2304x2304 pixels, 100 fps @ 2304x2048 pixels, 80% QE, 6.5 µm pixel size)
- Dual-camera system for simultaneous dual-color detection (e.g. GFP/RFP, RFP/SiR, GFP/SiR, upon request)
Software
- NIS-Elements AR (Advanced Research) including modules for:
- JOBS (automation)
- Deconvolution
- NIS.ai (Artificial Intelligence): denoise, clarify, enhance, segment, convert
Special
- Nipkow CSU-W for large FOV 1024x1024 px [size in µm for 10x]
- Uniform spinning disk illumination
- Enhanced signal-to-noise ratio compared to CSU-X
- 2 cameras for simultaneous dual-color imaging
- Fast emission filter wheel for clean multi-color imaging
- Ultra-fast laser switching for triggered multicolor acquisition
- SoRa-mode: Enhanced resolution (1.4x) in spinning disk mode
- Automatic water immersion dispenser (WID)
Emission Filters
| Filter | λ/Bandwidth |
| Quadband (DAPI, GFP, mCherry, Cy5) | 440/40 521/21 607/34 700/45 |
| DAPI | 447/60 |
| CFP | 472/30 |
| GFP | 525/50 |
| YFP | 542/27 |
| mCherry | 600/50 |
| Cy5 | 685/40 |
Objectives
| Mag. | Type | Immer. | NA | WD [μm] | Info |
| 10x (not installed) | Plan Achroat | Air | 0.45 | 4,000 | DIC N1 λ OFN25 |
| 40x | Apo LWD | Water | 1.15 | 600 | DIC N2 λS |
| 60x | Plan Apo | Water | 1.2 | 310-280 | DIC N2 VC OFN25 |
| 60x | Plan Apo | Oil | 1.4 | 130 | DIC N2 λ OFN25 |
| 25x | Plan Apo | Silicone | 1.05 | 550 | DIC N2 λS OFN25 |
| 40x | Plan Apo | Silicone | 1.25 | 300 | DIC N2 λS OFN25 |
| 100x | Plan Apo | Silicone | 1.35 | 300-290 | SR HP DIC N2 λS OFN22 |
Comments: New users - access upon training request here.
Name: SR 201 - big meeting point (SupraFAB)
Resource type: Room
Provider: SupraFAB
Affiliations: FU Berlin
Description: Large seminar room with tables and about 45 seats (more may be possible), equipped with a beamer.
Comments: 45 seats (more may be possible), with tables, equipped with a beamer.
Recurring bookings are possible; please remember to define the end date!
! Bookings become valid only after approval by the staff !
Name: AMBIO ZEISS Lattice Lightsheet 7
Resource type: Microscope
Provider: AMBIO
Affiliations: Charite
Description:
Imaging Modes
- Transmitted light with oblique contrast for sample positioning and overview
- Reflected light and epifluorescence with laser for beam adjustment and fluorescence light sheet imaging
- ibidi stage top incubation system (temperature, CO2, humidity) and integrated auto-immersion mechanism enable unattended long-term experiments
- Photo-manipulation: 405 and 473 nm and lasers for FRAP and photo-activation/photo-switch and 780 nm laser for ablation experiments
Acquisition
- Speed: Volume: 3 Vol/s @300 μm x 50 μm x 20 μm, plane: 400 frames/s @300 μm x 20 μm
- Penetration depth up to 80 - 200 μm
Illumination
- LED (white & red) for transmitted light
- Laser for reflected light & epi-fluorescence: 488 nm (2 mW), 561 nm (2 mW), 640 nm (1 mW)
Detectors
- pco.edge 4.2 CLHS sCMOS camera (2048x2048 pixels, 300 x 300 µm, 100 fps@16-bit full frame, 82% QE, 6.5 µm pixel size)
Software
- ZEN 3.5 (blue edition)
- Lattice Lightsheet Processing Module (deskewing, coverglass transformation)
- arivis Vision4D® software for advanced stitching, channel shift, high resolution volume rendering, and powerful deconvolution
Special
- Ultra-fast live 3D subcellular imaging with minimal phototoxicity and bleaching
- Near-isotropic resolution along the X, Y, and Z axes
- Using standard coverslips, 35 mm cell culture imaging dishes or well plates
- 5-axes stage tilt-able with high precision in X and Y
- Automatic water immersion dispenser
Objectives/Optics
- Illumination (light sheet): 13.3x / NA 0.4
- Detection: 44.83x / NA 1.0
Filters
| Filter (λ, Bandwidth) | Sample Fluorophore |
| LBF 405/ 488/ 561/ 642 | All, blocks excitation laser |
| BP 495-550/ BP 570-620 | GFP, mCherry |
| BP 495-550 +LP655 | GFP, Cy5 |
| BP 570-620 +LP655 | mCherry, Cy5 |
| BP 495-590 | GFP, YFP, mCherry |
| EF LP 570 | mCherry, Cy5 |
Comments: New users - access upon training request here.
Name: AMBIO ZEISS Lattice Lightsheet 7 Analysis Workstation
Resource type: Workstation
Provider: AMBIO
Affiliations: Charite
Description:
AMBIO ZEISS Lattice Lightsheet 7 Analysis Workstation
Hardware
- CPU: Intel Xeon E5-2630 v4 @ 2x 2.20 GHz, 256 GB RAM
- Graphic: NVIDIA Quadro RTX 6000, GPU Memory 24 GB RAM
- Hard drives: HDD 36 TB, 2x SSD 240 GB
Software
- Windows 10 64-bit System
- ZEISS ZEN 3.5 (blue edition)
- Lattice Lightsheet Processing Module (deskewing, coverglass transformation)
- arivis Vision 4D software mudule for advanced stitching, channel shift, high resolution volume rendering, and powerful deconvolution
- Fiji
Comments: Access upon request of analysis project here.
Name: 7th PluriCore Meeting 29 Feb - 1 Mar 2024
Resource type: Event
Provider: BIH Core Unit pluripotent Stem Cells & Organoids
Affiliations: Charite, BIH
Description:
Name: EEG-NIRS-System
Resource type: Equipment
Provider: Shared Equipment at Charité
Affiliations: Charite
Description: This EEG-NIRS system provides the ideal equipment for neuroimaging in developmental populations. Both EEG and NIRS components have been previously approved in developmental research and can be used in their combined or single application.
Electroencephalograpy (EEG):
actiCHampPlus 32 (8+) system with active electrodes (Brain Products GmbH)
Near-infrared spectroscopy (NIRS):
NIRScout Model 812 & NIRScout S Core System Unit (NIRx Medical Technologies LLC)
Comments: If you would like to use the EEG and/or NIRS equipment in our development laboratory, please contact us via email (see contacts).
Name: EnviroESCA
Resource type: Spectroscopy
Provider: Surface analytics unit - BCP
Affiliations: FU Berlin
Description:
Surface elemental analysis at near ambient/UHV conditions.
Booking of the EnviroESCA is restricted to trained key users of the working groups. If you are destined to become a key user for this device for your working group, please contact the resource admin(s).
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to key users and are possible at working days. The minimum booking time is 3 hours.
!Bookings are possible only for trained users!
Name: IONTOF M6
Resource type: Mass Spectrometer
Provider: Surface analytics unit - BCP
Affiliations: FU Berlin
Description: Surface analysis at near ambient/UHV conditions.
Booking of the IONTOF M6 is restricted to trained key users of the working groups. If you are destined to become a key user for this device for your working group, please contact the resource admin(s). Training can be also requested from the timeline view in the scheduler (top right, booklet symbol).
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained key users and are possible at working days. Minimum booking time 3 hours.
Sample preparation should be discussed with the measuring person in advance.
!Bookings are possible only for trained users!
Name: TGA 8000
Resource type: Gravimetric analysis
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Thermal gravimetric analysis (heating of the sample rt-1200 °C.)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Name: IR Spectrum Two
Resource type: Spectrometer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description:
Fourier-transform Infrared Spectroscopy (IR spectrum from 4000-650 cm-1.)
- ATR stage available
- Cuvette holder available
- Demountable cell available
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days. Minimum booking time 1 hour.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Name: UV-Vis Lambda 365
Resource type: Spectrometer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: UV-Vis Spectroscopy (UV spectrum from 190-1100 nm)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days. Minimum booking time 1 hour.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Name: MicroCal PEAQ-ITC
Resource type: Calorimeter
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Isothermal titration calorimeter
Name: MicroCal VP-ITC
Resource type: Calorimeter
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: isothermal titration calorimeter
Name: MPT-2 Mehrzweck Titrator
Resource type: Particle size analyzer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Titrator for atomated DLS measurements
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Name: Zetasizer Ultra "new"
Resource type: Particle size analyzer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Multi-Angle dynamic light Scattering (MADLS), Zeta potential
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Name: Zetasizer NanoZS "red"
Resource type: Particle size analyzer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: dynamic light scattering with possibility to do zeta-potential measurements
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Name: Fluorescence FL 6500
Resource type: Spectrometer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Fluorescence Spectroscopy (excitation and emission wavelength range up to 900 nm)
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days. Minimum booking time 1 hour.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Calibration performed 18th March 2024, excitation was off by ~10 nm.
Name: QCM-D (Quartz Crystal Microbalance)
Resource type: Other Instruments
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: A quartz crystal microbalance with dissipation monitoring (QCM-D) is a type of quartz crystal microbalance (QCM) based on the ring-down technique. It is used in interfacial acoustic sensing. Its most common application is the determination of a film thickness in a liquid environment (e.g., adsorbed protein layer, SAMs). It can be used to investigate further properties of the sample, most notably the layer's softness.
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Name: Proteomic MS Analysis of Biomaterials
Resource type: Mass Spectrometer
Provider: Imaging Mass Spectrometry Unit
Affiliations: Charite, BIH
Description: Proteomic characterization of therapeutic biomaterials (ECM, scaffolds and patches), tissue biopsies by using high standardized and robust LC-Based Mass Spectrometry.
Name: Proteomics MS-Analysis of therapeutic cell products
Resource type: Mass Spectrometer
Provider: Imaging Mass Spectrometry Unit
Affiliations: Charite, BIH
Description: Proteomic characterization of therapeutic cell products (e.g., Immune cells) by using high standardized and robust LC-Based Mass Spectrometry.
Name: Imaging Mass Spectrometry of FFPE Tissue section
Resource type: Imaging
Provider: Imaging Mass Spectrometry Unit
Affiliations: Charite, BIH
Description: In situ tissue assessment of spatial molecular signatures by MALDI-Mass Spectrometry Imaging (MALDI-MSI) to aid in the development of classifiers for therapy stratification and diagnosis/prognosis. This workflow includes: 1) tissue slicing, 2) tissue pre-preparation 3), MALDI-MSI analyses and chemical staining (e.g., HE for data co-registration).
Name: Seminar Room 001
Resource type: Room
Provider: Fachbereich Veterinärmedizin
Affiliations: FU Berlin
Description: Large Seminar Room
Comments:
Recurring bookings are possible; please remember to define the end date!
! Request based booking !
Name: Seminar Room 114
Resource type: Room
Provider: Fachbereich Veterinärmedizin
Affiliations: FU Berlin
Description: Small Seminar Room
Comments: 15 seats (more may be possible), equipped with a smartboard.
Recurring bookings are possible; please remember to define the end date!
! Request based booking !
Name: Idea office appointment
Resource type: Meeting Room
Provider: Idea Office
Affiliations: Charite, BIH
Description: Booking a meeting to discuss:
- Contacts to Charité-internal Idea-Office
- Information on "patents"
- Information on intellectual property rights – queries on contract administration and management
- Information on subsidiary programs and funds
- Assistance in filing an application
- Contact to companies
- Information on "spin-off creation"
- Information on "regulatory affairs"
Comments: Please select a 15 Minute slot. If the timeslot does not work for you please contact us (bettina.otto@charite.de and thomas.wallach@charite.de)
Name: Register my group with FB Veterinärmedizin
Resource type: Administration
Provider: Fachbereich Veterinärmedizin
Affiliations: FU Berlin
Description:
If your group is not yet installed in OpenIRIS, it will be created
concurrently, if your group is from FUB. External groups, however, must
be created by their home institution before access to FB Veterinärmedizin can be
provided.
In case of a representative puting the request, the group head must sign the form, which thereafter must be sent to Benjamin-F. Hempel (benjamin.hempel@fu-berlin.de) or Andreas Nerlich (andreas.nerlich@fu-berlin.de). Please do also appoint a second group admin that can take over administrative tasks for the group in OpenIRIS.
Comments: Group access to FB Veterinärmedizin is necessary to allow all group members to book resources of the core facility.
Preferably, the request should be put by the group head.
Name: Leica Stellaris 8
Resource type: Microscope
Provider: Shared Equipment at Charité
Affiliations: Charite
Description:
Imaging modes
- Scanning confocal (including XZ direction scans)
- Wide field (DAPI, FITC, YFP, RHOD, TXR)
- Video-imaging up to 60 fps
- TauSense, TauSeparation, TauGating: functional imaging based on arrival times
- FRAP
- Live-cell incubator (temperature, CO2, humidity)
- Spectral scans
- Lightning mode (near super-resolution)
Lasers and max. intensities out of 60x oil objective
- 405 nm
- White light laser: 440-700 nm (pulsed)
Emission Filters
| Filter | λ/Bandwidth |
| DAPI | 460/50 |
| FITC | 527/30 |
| YFP | 535/30 |
| RHOD | 585/40 |
| TXR | 630/75 |
Scanner
- AOBS scanner
Detectors
- 2x Power Hybrid Detektor HyD S
- 2x Power Hybrid Detektor HyD X
- Transmission light detector (DIC, phase)
- Spectral detection
Objectives
| Mag. | Type | Immer. | NA | WD [μm] | Info |
| 10x | HC PL FLUOTAR | Air | 0.3 | 11,000 | |
| 20x | HC PL FLUOTAR | Air | 0.55 | ||
| 20x | HC PL APO | Water (multi imm) | 0.75 | 670 | |
| 40x | HC PL APO | Oil | 1.30 | 240 | |
| 63x | HC PL APO | Water | 1.20 | 300 | motCorr |
| 2.5x | HC PL FLUOTAR | Air | 0.07 | 9,200 |
Special
- Super Z-galvo stage
- High precision LMT scanning stage
- Automatic water immersion for long-term imaging
- Resonant scanner for fast imaging
Comments: To get access to the microscope please click on the request access icon.
Name: STED Abberior Facility Line
Resource type: Microscope
Provider: AMBIO
Affiliations: Charite
Description:
Name: Sigma 6-16KS
Resource type: Centrifuge
Provider: Eigler Lab
Affiliations: FU Berlin
Description:
Refrigerated benchtop centrifuge.
THE USE OF THE CENTRIFUG HAS TO BE NOTED IN THE PHYSICAL LOG BOOK
Name: SONOPULS HD-3100
Resource type: Sonicator
Provider: Eigler Lab
Affiliations: FU Berlin
Description: Ultrasonic homogeniser
Name: Thermo Q Exactive HF (LC-MS)
Resource type: Mass Spectrometer
Provider: Bio Mass Spectrometry unit - BCP
Affiliations: FU Berlin
Description: hybrid quadrupole-Orbitrap mass spectrometer
Name: bioMS measurements (bioMS - BSM)
Resource type: Mass Spectrometry
Provider: Bio Mass Spectrometry unit - BCP
Affiliations: FU Berlin
Description: Every request demands a brief description of the sample and the preparation protocol, and must be approved by the group head of the requester. Only after this confirmation, the service can be initiated. We will then, together with the requester, decide on the
necessary steps prior to the data acquisition.
Comments:
Request of a bioMS measurement project is open to all users.
Name: Bruker D8 Venture
Resource type: X-ray Diffractometer
Provider: X-Ray diffraction unit - BCP
Affiliations: FU Berlin
Description: This Bruker D8 Venture single crystal diffractometer is available for non-assisted measurements for trained persons from FUB. It is equipped with two x-ray sources (Cu and Mo), a Photon II detector, and an oxford cryostream 700.
Comments: Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible in three predetermined blocks.
Name: OMERO Collab Group Request
Resource type: Request
Provider: OMERO Microscopy Image Data Management
Affiliations: Charite, BIH
Description: Request to create a collaboration group in OMERO.
Comments:
Request to create a collaboration group in OMERO.
Name: Circular Dichroism Spectrometer (Olis DSM 20)
Resource type: Spectrometer
Provider: Analytics Lab SupraFAB - BCP
Affiliations: FU Berlin
Description: Olis DSM 20 Circular Dichroism Spectrometer
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible at working days. Minimum booking time 1 hour.
Booking through OpenIris as well as filling the log book are required for the tracking the users of the device.
!Bookings are possible only for trained users!
To request a training click on any time and write in comments the responsible person who gave you the introduction
Name: Kibron MicroTrough XS
Resource type: Coater
Provider: Eigler Lab
Affiliations: FU Berlin
Description: Langmuir-Blodgett trough
Name: Glovebox Surface Analytics Lab 029
Resource type: Lab Space
Provider: Surface analytics unit - BCP
Affiliations: FU Berlin
Description:
Preparation, handling and storage of air- or moisture-sensitive samples under nitrogen atmosphere.
Booking of the glovebox is restricted to trained key users. If you are destined to become a key user for this device for your working group, please contact the resource admin(s).
Comments:
Please note the restricted access to this instrument!
Bookings are restricted to key users and are possible at working days.
!Bookings are possible only for trained users!
Name: Tube Furnace Nabertherm
Resource type: Heating
Provider: Eigler Lab
Affiliations: FU Berlin
Description: Nabertherm with B410 controller
Comments: Temperature 1200 °C
Name: Axio Imager Vario
Resource type: Microscope
Provider: Core Facility for Electron Microscopy
Affiliations: Charite
Description: Suitable for LM questions from semithin section to IFM.
Name: MicroCT RX-Solutions
Resource type: Other Instruments
Provider: Dentistry Research Groups
Affiliations: Charite
Description: With the EasyTom S, one simple scan allows you to see through your parts: internal/external structures, defects, porosities... visualize, measure, and analyze whatever the sample.
Name: 1. Request to register research group in OpenIRIS
Resource type: Administration
Provider: Administration Open IRIS Charité & BIH
Affiliations: Charite, BIH
Description: Please submit this form to have your research group registered in OpenIRIS.
Comments: Form not for requesting to join a group. Please navigate to BROWSE > GROUPS, set filter to ALL and search for PI surname.
Name: 2. Add a cost center / 'Innenauftrag' to a research group
Resource type: Administration
Provider: Administration Open IRIS Charité & BIH
Affiliations: Charite, BIH
Description: You can submit this form to have a cost center added to a research group that is already registered in OpenIRIS.
Name: 3. Add / change group admin for research group
Resource type: Administration
Provider: Administration Open IRIS Charité & BIH
Affiliations: Charite, BIH
Description: You can submit this form to request additional admins be added to a research group.
Name: Aurora "Paris" - Full Spectrum Analyzer
Resource type: Flow Cytometry Analyzer
Provider: Flow & Mass Cytometry Core Facility
Affiliations: Charite, BIH
Description: 5-Laser full spectrum analyzer
Comments:
You need to be registered and trained to use this instrument.
Cancel your booking at least 24 hours ahead of your booking to avoid charges.
Name: Device 3 Access Form
Resource type: Form
Provider: Test Provider
Affiliations: Charite, BIH
Description: Access form to get Device 3 access
Comments: Please submit form to get access to Device 3
Name: Service measurement XPS (SA unit - BSM)
Resource type: Spectrometer
Provider: Surface analytics unit - BCP
Affiliations: FU Berlin
Description: abc
Comments: You apply for assisted surface characterization (service measurement) at the Surface Analytics Unit. The surface characterization of compounds/materials is charged. By this request you accept the obligation of coverage of the costs for the measurements. User fees are listed here: www.biosupramol.de. You will be contacted as soon as possible to discuss further proceeding.
Name: STOE IPDS 2T
Resource type: X-ray Diffractometer
Provider: X-Ray diffraction unit - BCP
Affiliations: FU Berlin
Description: This Oxford Diffraction Xcalibur Nova O single crystal diffractometer is available for non-assisted measurements for trained persons from FUB. It is equipped a Cu x-ray sources, an Onyx CCD detector, and an Oxford CryojetXL.
Comments: Please note the restricted access to this instrument!
Bookings are restricted to trained users and are possible in three predetermined blocks.
Name: 1a. Registration of a research group
Resource type: Request
Provider: Administration OpenIRIS HU Berlin
Affiliations: HU Berlin
Description:
Please submit this form in order to register a research group in OpenIRIS.
Please check before submitting the form if the group is already existing in OpenIRIS: Navigate to BROWSE > GROUPS, and search in the search field for the last name of the group leader. Check if all filters have been removed.
Please note that it is sufficient to fill out the form in either English or German. You do not need to complete both versions. Please choose the language that is most convenient for you.
Name: 2a. Ask a question
Resource type: Request
Provider: Administration OpenIRIS HU Berlin
Affiliations: HU Berlin
Description:
Pleas fill in this form if you would like to ask the HU OpenIRIS coordinator a question regarding OpenIRIS.
Please note that it is sufficient to fill out the form in either English or German. Please choose the language that is most convenient for you.
Name: 1b. Registrierung einer Forschungsgruppe
Resource type: Request
Provider: Administration OpenIRIS HU Berlin
Affiliations: HU Berlin
Description:
Bitte füllen Sie das Formular aus, wenn sie eine Arbeitsgruppe/Forschungsgruppe in OpenIRIS registrieren möchten.
Bitte überprüfen Sie vor dem Einreichen des Formulars, ob die Gruppe bereits in OpenIRIS existiert: Navigieren Sie dazu zu BROWSE> GROUPS und suchen Sie in dem Suchfeld nach dem Nachnamen des Gruppenleiters/ der Gruppenleiterin. Überprüfen Sie, ob alle Filter zuvor entfernt worden sind.
Es ist ausreichend das Formular in Englisch oder in Deutsch auszufüllen. Es ist nicht notwendig beide Versionen auszufüllen. Bitte wählen Sie die Sprache, die Ihnen am besten zusagt.
Name: 2b. Frage stellen
Resource type: Request
Provider: Administration OpenIRIS HU Berlin
Affiliations: HU Berlin
Description:
Bitte schreiben Sie eine Nachricht über dieses Formular, wenn Sie eine Frage zu OpenIRIS haben.
Es ist ausreichend das Formular in Englisch oder in Deutsch auszufüllen. Bitte wählen Sie die Sprache, die Ihnen am besten zusagt.